Cited by 304Neuroscience and Neuropharmacology ResearchAmino Acid Enzymes and MetabolismNicotinic Acetylcholine Receptors Study

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NMDA Receptor Content of Synapses in Stratum Radiatum of the Hippocampal CA1 Area

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Racca, Claudia, Stephenson, F. Anne, Streit, Peter, Roberts, J. David B., Somogyi, Peter·2000·The Journal of Neuroscience

DOI 10.1523/jneurosci.20-07-02512.2000PMC PMC6772245

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Abstract

Glutamate receptors activated by NMDA (NMDARs) or AMPA (AMPARs) are clustered on dendritic spines of pyramidal cells. Both the AMPAR-mediated postsynaptic responses and the synaptic AMPAR immunoreactivity show a large intersynapse variability. Postsynaptic responses mediated by NMDARs show less variability. To assess the variability in NMDAR content and the extent of their coexistence with AMPARs in Schaffer collateral–commissural synapses of adult rat CA1 pyramidal cells, electron microscopic immunogold localization of receptors has been used. Immunoreactivity of NMDARs was detected in virtually all synapses on spines, but AMPARs were undetectable, on average, in 12% of synapses. A proportion of synapses had a very high AMPAR content relative to the mean content, resulting in a distribution more skewed toward larger values than that of NMDARs. The variability of synaptic NMDAR content [coefficient of variation (CV), 0.64–0.70] was much lower than that of the AMPAR content (CV, 1.17–1.45). Unlike the AMPAR content, the NMDAR content showed only a weak correlation with synapse size. As reported previously for AMPARs, the immunoreactivity of NMDARs was also associated with the spine apparatus within spines. The results demonstrate that the majority of the synapses made by CA3 pyramidal cells onto spines of CA1 pyramids express both NMDARs and AMPARs, but with variable ratios. A less-variable NMDAR content is accompanied by a wide variability of AMPAR content, indicating that the regulation of expression of the two receptors is not closely linked. These findings support reports that fast excitatory transmission at some of these synapses is mediated by activation mainly of NMDARs.

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Authors: Racca, C., Stephenson, F. A., Streit, P., Roberts, J. D. B., & Somogyi, P.
Affiliations: University of Oxford (GB)
University of London (GB)
University of Zurich (CH)
ORCID: Racca, Claudia
Stephenson, F. Anne
Venue: The Journal of Neuroscience
Publisher: Society for Neuroscience
Published: 2000-04-01
Volume: 20
Issue: 7
Pages: 2512-2522
ISSN: 0270-6474 (ISSN-L)
1529-2401
DOI: 10.1523/jneurosci.20-07-02512.2000
PMC: PMC6772245
OpenAlex: W2144874949
Cited by: 304
References: 59
Citations by year: 2026: 3 · 2025: 7 · 2024: 14 · 2023: 7 · 2022: 9 · 2021: 8 · 2020: 10 · 2019: 8 · 2018: 9 · 2017: 5 · 2016: 10 · 2015: 10 · 2014: 6 · 2013: 6 · 2012: 14
FWCI: 8.02
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-sa
creativecommons.org/licenses/by-nc-sa/4.0/ (vor, 183 d)
Locations: Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
UCL Discovery (University College London) (submittedVersion)
Newcastle University ePrints (Newcastle Univesity)
Funders: National Institutes of Health
National Institute on Deafness and Other Communication Disorders
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
Keywords: NMDA receptor, AMPA receptors, Schaffer collateral synapses, CA1 pyramidal neurons, CA3 pyramidal cells, dendritic spines, spine apparatus, synapse size, hippocampus
MeSH terms: Animals, Cell Line, Dendrites, Humans, Immunohistochemistry, Synapses, Receptors, N-Methyl-D-Aspartate, Rats, Wistar, Pyramidal Cells, Receptors, AMPA, Rats

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NMDA Receptor Content of Synapses in Stratum Radiatum of the Hippocampal CA1 Area

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