Different modes of expression of AMPA and NMDA receptors in hippocampal synapses

Takumi, Yutaka; Ramírez-León, Vania; Laake, Petter; Rinvik, Eric; Ottersen, Ole P.

doi:10.1038/10172

Cited by 675Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsNicotinic Acetylcholine Receptors Study

Different modes of expression of AMPA and NMDA receptors in hippocampal synapses

Takumi, Yutaka, Ramírez-León, Vania, Laake, Petter, Rinvik, Eric, Ottersen, Ole P.·1999·Nature Neuroscience

DOI 10.1038/10172

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Abstract

Postembedding immunogold labeling was used to determine the relationship between AMPA and NMDA receptor density and size of Schaffer collateral-commissural (SCC) synapses of the adult rat. All SCC synapses expressed NMDA receptors. AMPA and NMDA receptors were colocalized in at least 75% of SCC synapses; the ratio of AMPA to NMDA receptors was a linear function of postsynaptic density (PSD) diameter, with AMPA receptor number dropping to zero at a PSD diameter of approximately 180 nm. These findings indicate that 'silent' SCC synapses are smaller than the majority of SCC synapses at which AMPA and NMDA receptors are colocalized. Thus synapse size may determine important properties of SCC synapses.

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The data are consistent with the correlation between AMPA receptor immunoreactivity and the size of PSD, because the size of PSD is nearly proportional to the spine-head volume.
“These data are consistent with the correlation between AMPA receptor immunoreactivity and the size of PSD19, 20, because the size of PSD is nearly proportional to the spine-head volume13.”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [20]
Regions of glutamate sensitivity tended to be asymmetrically localized to one portion of a spine.
“Regions of glutamate sensitivity tended to be asymmetrically localized to one portion of a spine (Figs. 3h and 4c–j), consistent with the predominant localization of AMPA receptors within PSDs with a diameter of 0.1 to 0.5μm13, 19, 20.”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [20]
On average there are approximately 250 PSD-95 molecules in the PSD and approximately 50 AMPARs, although this number is highly variable.
“On average there are ∼250 PSD-95 molecules in the PSD (65) and, on average, ∼50 AMPARs, although this number is highly variable (143, 290, 291).”
From Synaptic memory and CaMKII (2023) · cites this paper as [291]

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Authors: Takumi, Y., Ramírez-León, V., Laake, P., Rinvik, E., & Ottersen, O. P.
Affiliations: Hirosaki University (JP)
University of Oslo (NO)
Corresponding author: Ottersen, Ole P.
ORCID: Takumi, Yutaka
Ottersen, Ole P.
Venue: Nature Neuroscience
Publisher: Springer Science and Business Media LLC
Published: 1999-07
Volume: 2
Issue: 7
Pages: 618-624
ISSN: 1097-6256 (ISSN-L)
1546-1726
DOI: 10.1038/10172
OpenAlex: W1597908762
Cited by: 675
References: 0
Citations by year: 2026: 2 · 2025: 11 · 2024: 12 · 2023: 19 · 2022: 21 · 2021: 18 · 2020: 14 · 2019: 18 · 2018: 30 · 2017: 20 · 2016: 28 · 2015: 19 · 2014: 25 · 2013: 25 · 2012: 22
FWCI: 12.31
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Neuroscience (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
Keywords: AMPA receptors, NMDA receptor, hippocampus, postsynaptic density, synapse size, receptor colocalization, silent synapses, synaptic plasticity
MeSH terms: Animals, Hippocampus, Male, Regression Analysis, Synapses, Receptors, N-Methyl-D-Aspartate, Microscopy, Immunoelectron, Rats, Wistar, Receptors, AMPA, Rats

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Different modes of expression of AMPA and NMDA receptors in hippocampal synapses

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