Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

Hydrogel: Preparation, characterization, and applications: A review

Ahmed, E. M. (2015) · Journal of Advanced Research · doi:10.1016/j.jare.2013.07.006 · Cited by 5,800

Photothermal therapy and photoacoustic imaging via nanotheranostics in fighting cancer

Liu, Y., Bhattarai, P., Dai, Z., & Chen, X. (2019) · Chemical Society Reviews · doi:10.1039/c8cs00618k · Cited by 2,904

Applications of ultrasound in food technology: Processing, preservation and extraction

Chemat, F., Zill-e-Huma, & Khan, M. K. (2011) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2010.11.023 · Cited by 2,533

Printing ferromagnetic domains for untethered fast-transforming soft materials

Kim, Y., Yuk, H., Zhao, R., Chester, S. A., & Zhao, X. (2018) · Nature · doi:10.1038/s41586-018-0185-0 · Cited by 2,183

Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures

POLGE, C., SMITH, A. U., & PARKES, A. S. (1949) · Nature · doi:10.1038/164666a0 · Cited by 2,081

Freezing of living cells: mechanisms and implications

Mazur, P. (1984) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1984.247.3.c125 · Cited by 1,816

Hydrogel ionotronics

Yang, C., & Suo, Z. (2018) · Nature Reviews Materials · doi:10.1038/s41578-018-0018-7 · Cited by 1,709

Emerging Droplet Microfluidics

Shang, L., Cheng, Y., & Zhao, Y. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00848 · Cited by 1,584

Cryobiology: The Freezing of Biological Systems: The responses of living cells to ice formation are of theoretical interest and practical concern.

Mazur, P. (1970) · Science · doi:10.1126/science.168.3934.939 · Cited by 1,402

Stem cell gene expression programs influence clinical outcome in human leukemia

Eppert, K., Takenaka, K., Lechman, E. R., Waldron, L., Nilsson, B., van Galen, P., Metzeler, K. H., Poeppl, A., Ling, V., Beyene, J., et al. (2011) · Nature Medicine · doi:10.1038/nm.2415 · Cited by 1,041

A two-factor hypothesis of freezing injury

Mazur, P., Leibo, S. P., & Chu, E. H. Y. (1972) · Experimental Cell Research · doi:10.1016/0014-4827(72)90303-5 · Cited by 969

Prevention of Freezing Damage to Living Cells by Dimethyl Sulphoxide

LOVELOCK, J. E., & BISHOP, M. W. H. (1959) · Nature · doi:10.1038/1831394a0 · Cited by 966

Chemo-photothermal therapy combination elicits anti-tumor immunity against advanced metastatic cancer

Nam, J., Son, S., Ochyl, L. J., Kuai, R., Schwendeman, A., & Moon, J. J. (2018) · Nature Communications · doi:10.1038/s41467-018-03473-9 · Cited by 808

The role of intracellular freezing in the death of cells cooled at supraoptimal rates

Mazur, P. (1977) · Cryobiology · doi:10.1016/0011-2240(77)90175-4 · Cited by 747

Freezing Resistance in Some Antarctic Fishes

DeVries, A. L., & Wohlschlag, D. E. (1969) · Science · doi:10.1126/science.163.3871.1073 · Cited by 691

The cryobiology of cryosurgical injury

Hoffmann, N. E., & Bischof, J. C. (2002) · Urology · doi:10.1016/s0090-4295(02)01683-7 · Cited by 592

The promise of organ and tissue preservation to transform medicine

Giwa, S., Lewis, J. K., Alvarez, L., Langer, R., Roth, A. E., Church, G. M., Markmann, J. F., Sachs, D. H., Chandraker, A., Wertheim, J. A., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3889 · Cited by 539

Mechanisms of Cryoinjury in Living Cells

Gao, D., & Critser, J. K. (2000) · ILAR Journal · doi:10.1093/ilar.41.4.187 · Cited by 533

Cryoprotectant Toxicity: Facts, Issues, and Questions

Best, B. P. (2015) · Rejuvenation Research · doi:10.1089/rej.2014.1656 · Cited by 523

Intracellular trehalose improves the survival of cryopreserved mammalian cells

Eroglu, A., Russo, M. J., Bieganski, R., Fowler, A., Cheley, S., Bayley, H., & Toner, M. (2000) · Nature Biotechnology · doi:10.1038/72608 · Cited by 520

Mimicry of ice structure by surface hydroxyls and water of a β-helix antifreeze protein

Liou, Y.-C., Tocilj, A., Davies, P. L., & Jia, Z. (2000) · Nature · doi:10.1038/35018604 · Cited by 492

Cryopreservation and its clinical applications

Jang, T. H., Park, S. C., Yang, J. H., Kim, J. Y., Seok, J. H., Park, U. S., Choi, C. W., Lee, S. R., & Han, J. (2017) · Integrative Medicine Research · doi:10.1016/j.imr.2016.12.001 · Cited by 485

Recent Advances in Cell Membrane–Camouflaged Nanoparticles for Cancer Phototherapy

Zhen, X., Cheng, P., & Pu, K. (2019) · Small · doi:10.1002/smll.201804105 · Cited by 483

Cell membrane-derived nanomaterials for biomedical applications

Fang, R. H., Jiang, Y., Fang, J. C., & Zhang, L. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.02.041 · Cited by 468

Biomimetic anti-freezing polymeric hydrogels: keeping soft-wet materials active in cold environments

Jian, Y., Handschuh-Wang, S., Zhang, J., Lu, W., Zhou, X., & Chen, T. (2021) · Materials Horizons · doi:10.1039/d0mh01029d · Cited by 457

Probing the critical nucleus size for ice formation with graphene oxide nanosheets

Bai, G., Gao, D., Liu, Z., Zhou, X., & Wang, J. (2019) · Nature · doi:10.1038/s41586-019-1827-6 · Cited by 442

Emerging Technologies for Food Processing

(2005) · Elsevier eBooks · doi:10.1016/b978-0-12-676757-5.x5000-2 · Cited by 430

Ice-Binding Proteins and Their Function

Bar Dolev, M., Braslavsky, I., & Davies, P. L. (2016) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060815-014546 · Cited by 426

Ice-binding proteins: a remarkable diversity of structures for stopping and starting ice growth

Davies, P. L. (2014) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2014.09.005 · Cited by 422

Supramolecular nanofibrillar hydrogels as highly stretchable, elastic and sensitive ionic sensors

Zhang, X., Sheng, N., Wang, L., Tan, Y., Liu, C., Xia, Y., Nie, Z., & Sui, K. (2019) · Materials Horizons · doi:10.1039/c8mh01188e · Cited by 412

Anchored clathrate waters bind antifreeze proteins to ice

Garnham, C. P., Campbell, R. L., & Davies, P. L. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100429108 · Cited by 399

Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs

Yeste, M. (2016) · Theriogenology · doi:10.1016/j.theriogenology.2015.09.047 · Cited by 391

Control of ice nucleation: freezing and antifreeze strategies

Zhang, Z., & Liu, X.-Y. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00626a · Cited by 384

A New 3D Printing Strategy by Harnessing Deformation, Instability, and Fracture of Viscoelastic Inks

Yuk, H., & Zhao, X. (2018) · Advanced Materials · doi:10.1002/adma.201704028 · Cited by 381

Thermodynamics and kinetics of intracellular ice formation during freezing of biological cells

Toner, M., Cravalho, E. G., & Karel, M. (1990) · Journal of Applied Physics · doi:10.1063/1.345670 · Cited by 378

Restorative effects of human neural stem cell grafts on the primate spinal cord

Rosenzweig, E. S., Brock, J. H., Lu, P., Kumamaru, H., Salegio, E. A., Kadoya, K., Weber, J. L., Liang, J. J., Moseanko, R., Hawbecker, S., et al. (2018) · Nature Medicine · doi:10.1038/nm.4502 · Cited by 342

Janus effect of antifreeze proteins on ice nucleation

Liu, K., Wang, C., Ma, J., Shi, G., Yao, X., Fang, H., Song, Y., & Wang, J. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1614379114 · Cited by 315

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

Deller, R. C., Vatish, M., Mitchell, D. A., & Gibson, M. I. (2014) · Nature Communications · doi:10.1038/ncomms4244 · Cited by 311

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

Manuchehrabadi, N., Gao, Z., Zhang, J., Ring, H. L., Shao, Q., Liu, F., McDermott, M., Fok, A., Rabin, Y., Brockbank, K. G. M., et al. (2017) · Science Translational Medicine · doi:10.1126/scitranslmed.aah4586 · Cited by 305

Bioinspired ultra-stretchable and anti-freezing conductive hydrogel fibers with ordered and reversible polymer chain alignment

Zhao, X., Chen, F., Li, Y., Lu, H., Zhang, N., & Ma, M. (2018) · Nature Communications · doi:10.1038/s41467-018-05904-z · Cited by 291

Stem cell medicine encounters the immune system

Bradley, J. A., Bolton, E. M., & Pedersen, R. A. (2002) · Nature Reviews Immunology · doi:10.1038/nri934 · Cited by 290

Cryopreservation of organs by vitrification: perspectives and recent advances

Fahy, G. M., Wowk, B., Wu, J., Phan, J., Rasch, C., Chang, A., & Zendejas, E. (2004) · Cryobiology · doi:10.1016/j.cryobiol.2004.02.002 · Cited by 287

Chitosan-Decorated Doxorubicin-Encapsulated Nanoparticle Targets and Eliminates Tumor Reinitiating Cancer Stem-like Cells

Rao, W., Wang, H., Han, J., Zhao, S., Dumbleton, J., Agarwal, P., Zhang, W., Zhao, G., Yu, J., Zynger, D. L., et al. (2015) · ACS Nano · doi:10.1021/nn506928p · Cited by 281

Graphene Oxide Restricts Growth and Recrystallization of Ice Crystals

Geng, H., Liu, X., Shi, G., Bai, G., Ma, J., Chen, J., Wu, Z., Song, Y., Fang, H., & Wang, J. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201609230 · Cited by 278

Stretchable living materials and devices with hydrogel–elastomer hybrids hosting programmed cells

Liu, X., Tang, T.-C., Tham, E., Yuk, H., Lin, S., Lu, T. K., & Zhao, X. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1618307114 · Cited by 269

Manganese Dioxide Nanozymes as Responsive Cytoprotective Shells for Individual Living Cell Encapsulation

Li, W., Liu, Z., Liu, C., Guan, Y., Ren, J., & Qu, X. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201706910 · Cited by 257

Bioinspired Multifunctional Anti-icing Hydrogel

He, Z., Wu, C., Hua, M., Wu, S., Wu, D., Zhu, X., Wang, J., & He, X. (2020) · Matter · doi:10.1016/j.matt.2019.12.017 · Cited by 257

Mechanisms of intracellular ice formation

Muldrew, K., & McGann, L. E. (1990) · Biophysical Journal · doi:10.1016/s0006-3495(90)82568-6 · Cited by 256

Polymer mimics of biomacromolecular antifreezes

Biggs, C. I., Bailey, T. L., Graham, B., Stubbs, C., Fayter, A., & Gibson, M. I. (2017) · Nature Communications · doi:10.1038/s41467-017-01421-7 · Cited by 247

Bioinspired Materials for Controlling Ice Nucleation, Growth, and Recrystallization

He, Z., Liu, K., & Wang, J. (2018) · Accounts of Chemical Research · doi:10.1021/acs.accounts.7b00528 · Cited by 247

A Review of Novel and Innovative Food Freezing Technologies

James, C., Purnell, G., & James, S. J. (2015) · Food and Bioprocess Technology · doi:10.1007/s11947-015-1542-8 · Cited by 245

Nonequilibrium Antifreeze Peptides and the Recrystallization of Ice

Knight, C. A., Wen, D., & Laursen, R. A. (1995) · Cryobiology · doi:10.1006/cryo.1995.1002 · Cited by 240

Antifreeze (Glyco)protein Mimetic Behavior of Poly(vinyl alcohol): Detailed Structure Ice Recrystallization Inhibition Activity Study

Congdon, T., Notman, R., & Gibson, M. I. (2013) · Biomacromolecules · doi:10.1021/bm400217j · Cited by 237

Expansion of primitive human hematopoietic stem cells by culture in a zwitterionic hydrogel

Bai, T., Li, J., Sinclair, A., Imren, S., Merriam, F., Sun, F., O’Kelly, M. B., Nourigat, C., Jain, P., Delrow, J. J., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0601-5 · Cited by 228

From ice-binding proteins to bio-inspired antifreeze materials

Voets, I. K. (2017) · Soft Matter · doi:10.1039/c6sm02867e · Cited by 226

Antifreeze protein modulates cell survival during cryopreservation: mediation through influence on ice crystal growth.

Carpenter, J. F., & Hansen, T. N. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.19.8953 · Cited by 220

Supercooling enables long-term transplantation survival following 4 days of liver preservation

Berendsen, T. A., Bruinsma, B. G., Puts, C. F., Saeidi, N., Usta, O. B., Uygun, B. E., Izamis, M.-L., Toner, M., Yarmush, M. L., & Uygun, K. (2014) · Nature Medicine · doi:10.1038/nm.3588 · Cited by 205

The principles of ultrasound and its application in freezing related processes of food materials: A review

Cheng, X., Zhang, M., Xu, B., Adhikari, B., & Sun, J. (2015) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2015.04.015 · Cited by 205

Blocking rapid ice crystal growth through nonbasal plane adsorption of antifreeze proteins

Olijve, L. L. C., Meister, K., DeVries, A. L., Duman, J. G., Guo, S., Bakker, H. J., & Voets, I. K. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524109113 · Cited by 196

Tuning ice nucleation with counterions on polyelectrolyte brush surfaces

He, Z., Xie, W. J., Liu, Z., Liu, G., Wang, Z., Gao, Y. Q., & Wang, J. (2016) · Science Advances · doi:10.1126/sciadv.1600345 · Cited by 195

Biomanufacturing for clinically advanced cell therapies

Aijaz, A., Li, M., Smith, D., Khong, D., LeBlon, C., Fenton, O. S., Olabisi, R. M., Libutti, S., Tischfield, J., Maus, M. V., et al. (2018) · Nature Biomedical Engineering · doi:10.1038/s41551-018-0246-6 · Cited by 191

Vitrification versus slow freezing for human ovarian tissue cryopreservation: a systematic review and meta-anlaysis

Shi, Q., Xie, Y., Wang, Y., & Li, S. (2017) · Scientific Reports · doi:10.1038/s41598-017-09005-7 · Cited by 188

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

Kim, H., Lee, J., Hur, Y., Lee, C., Park, S.-H., & Koo, B.-W. (2017) · Marine Drugs · doi:10.3390/md15020027 · Cited by 186

Antifreeze Glycoproteins Bind Reversibly to Ice via Hydrophobic Groups

Mochizuki, K., & Molinero, V. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.7b13630 · Cited by 184

Oxidized Quasi‐Carbon Nitride Quantum Dots Inhibit Ice Growth

Bai, G., Song, Z., Geng, H., Gao, D., Liu, K., Wu, S., Rao, W., Guo, L., & Wang, J. (2017) · Advanced Materials · doi:10.1002/adma.201606843 · Cited by 182

Observation of ice-like water layers at an aqueous protein surface

Meister, K., Strazdaite, S., DeVries, A. L., Lotze, S., Olijve, L. L. C., Voets, I. K., & Bakker, H. J. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1414188111 · Cited by 179

The relevance of ice crystal formation for the cryopreservation of tissues and organs

Pegg, D. E. (2010) · Cryobiology · doi:10.1016/j.cryobiol.2010.02.003 · Cited by 175

Ice Recrystallization Inhibition and Molecular Recognition of Ice Faces by Poly(vinyl alcohol)

Budke, C., & Koop, T. (2006) · ChemPhysChem · doi:10.1002/cphc.200600533 · Cited by 173

Recent Advances in Food Thawing Technologies

Cai, L., Cao, M., Regenstein, J. M., & Cao, A. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12458 · Cited by 160

The Grand Challenges of Organ Banking: Proceedings from the first global summit on complex tissue cryopreservation

Lewis, J. K., Bischof, J. C., Braslavsky, I., Brockbank, K. G. M., Fahy, G. M., Fuller, B. J., Rabin, Y., Tocchio, A., Woods, E. J., Wowk, B. G., et al. (2015) · Cryobiology · doi:10.1016/j.cryobiol.2015.12.001 · Cited by 155

Precise targeting of POLR2A as a therapeutic strategy for human triple negative breast cancer

Xu, J., Liu, Y., Li, Y., Wang, H., Stewart, S., der Jeught, K. V., Agarwal, P., Zhang, Y., Liu, S., Zhao, G., et al. (2019) · Nature Nanotechnology · doi:10.1038/s41565-019-0381-6 · Cited by 154

Gold Nanorod Induced Warming of Embryos from the Cryogenic State Enhances Viability

Khosla, K., Wang, Y., Hagedorn, M., Qin, Z., & Bischof, J. C. (2017) · ACS Nano · doi:10.1021/acsnano.7b02216 · Cited by 150

Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers

Graham, B., Bailey, T. L., Healey, J. R. J., Marcellini, M., Deville, S., & Gibson, M. I. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201706703 · Cited by 143

Mimicking the Ice Recrystallization Activity of Biological Antifreezes. When is a New Polymer “Active”?

Biggs, C. I., Stubbs, C., Graham, B., Fayter, A. E. R., Hasan, M., & Gibson, M. I. (2019) · Macromolecular Bioscience · doi:10.1002/mabi.201900082 · Cited by 143

Toward a Molecular Understanding of the Mechanism of Cryopreservation by Polyampholytes: Cell Membrane Interactions and Hydrophobicity

Rajan, R., Hayashi, F., Nagashima, T., & Matsumura, K. (2016) · Biomacromolecules · doi:10.1021/acs.biomac.6b00343 · Cited by 140

Nanoparticle-Mediated Intracellular Delivery Enables Cryopreservation of Human Adipose-Derived Stem Cells Using Trehalose as the Sole Cryoprotectant

Rao, W., Huang, H., Wang, H., Zhao, S., Dumbleton, J., Zhao, G., & He, X. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b00655 · Cited by 139

Targeting Cryopreservation-Induced Cell Death: A Review

Bissoyi, A., Nayak, B., Pramanik, K., & Sarangi, S. K. (2014) · Biopreservation and Biobanking · doi:10.1089/bio.2013.0032 · Cited by 136

Microfluidics for cryopreservation

Zhao, G., & Fu, J. (2017) · Biotechnology Advances · doi:10.1016/j.biotechadv.2017.01.006 · Cited by 134

Metal–Organic Framework Nanoparticle-Assisted Cryopreservation of Red Blood Cells

Zhu, W., Guo, J., Agola, J. O., Croissant, J. G., Wang, Z., Shang, J., Coker, E. N., Motevalli, B., Zimpel, A., Wuttke, S., et al. (2019) · Journal of the American Chemical Society · doi:10.1021/jacs.9b00992 · Cited by 133

Alginate Hydrogel Microencapsulation Inhibits Devitrification and Enables Large‐Volume Low‐CPA Cell Vitrification

Huang, H., Choi, J. K., Rao, W., Zhao, S., Agarwal, P., Zhao, G., & He, X. (2015) · Advanced Functional Materials · doi:10.1002/adfm.201503047 · Cited by 126

Microfluidic Generation of Nanomaterials for Biomedical Applications

Zhao, X., Bian, F., Sun, L., Cai, L., Li, L., & Zhao, Y. (2019) · Small · doi:10.1002/smll.201901943 · Cited by 126

The effect of antifreeze glycopeptides on membrane potential changes at hypothermic temperatures

Rubinsky, B., Arav, A., Mattioli, M., & DeVries, A. L. (1990) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(05)80939-8 · Cited by 125

PHYSICAL FACTORS IMPLICATED IN THE DEATH OF MICROORGANISMS AT SUBZERO TEMPERATURES*

Mazur, P. (1960) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1960.tb49986.x · Cited by 123

Microfluidics for cryopreservation

Song, Y. S., Moon, S., Hulli, L., Hasan, S. K., Kayaalp, E., & Demirci, U. (2009) · Lab on a Chip · doi:10.1039/b823062e · Cited by 122

Total Synthesis of Homogeneous Antifreeze Glycopeptides and Glycoproteins

Wilkinson, B. L., Stone, R. S., Capicciotti, C. J., Thaysen‐Andersen, M., Matthews, J. M., Packer, N. H., Ben, R. N., & Payne, R. J. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201108682 · Cited by 121

Cell encapsulating droplet vitrification

Demirci, U., & Montesano, G. (2007) · Lab on a Chip · doi:10.1039/b705809h · Cited by 118

Intracellular Delivery of Trehalose for Cell Banking

Stewart, S., & He, X. (2018) · Langmuir · doi:10.1021/acs.langmuir.8b02015 · Cited by 118

Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse

Jin, B., Kleinhans, F. W., & Mazur, P. (2014) · Cryobiology · doi:10.1016/j.cryobiol.2014.03.005 · Cited by 117

Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes

Hornberger, K., Yu, G., McKenna, D. H., & Hubel, A. (2019) · Transfusion Medicine and Hemotherapy · doi:10.1159/000496068 · Cited by 113

The effects of cancer and cancer treatments on male reproductive function

Magelssen, H., Brydøy, M., & Fosså, S. D. (2006) · Nature Clinical Practice Urology · doi:10.1038/ncpuro0508 · Cited by 112

Cryopreservation: Vitrification and Controlled Rate Cooling

Hunt, C. J. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-6921-0_5 · Cited by 107

Incorporation of Antifreeze Proteins into Polymer Coatings Using Site-Selective Bioconjugation

Esser-Kahn, A. P., Trang, V. H., & Francis, M. B. (2010) · Journal of the American Chemical Society · doi:10.1021/ja103038p · Cited by 106

Application of microfluidic technologies to human assisted reproduction

Smith, G. D., & Takayama, S. (2017) · Molecular Human Reproduction · doi:10.1093/molehr/gaw076 · Cited by 106

Hydrogel Encapsulation Facilitates Rapid‐Cooling Cryopreservation of Stem Cell‐Laden Core–Shell Microcapsules as Cell–Biomaterial Constructs

Zhao, G., Liu, X., Zhu, K., & He, X. (2017) · Advanced Healthcare Materials · doi:10.1002/adhm.201700988 · Cited by 105

Off the shelf cellular therapeutics: Factors to consider during cryopreservation and storage of human cells for clinical use

Woods, E. J., Thirumala, S., Badhe-Buchanan, S., Clarke, D. M., & Mathew, A. J. (2016) · Cytotherapy · doi:10.1016/j.jcyt.2016.03.295 · Cited by 104

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

Wang, J., Zhao, G., Zhang, Z., Xu, X., & He, X. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.01.026 · Cited by 103

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

Gao, Z., Ring, H. L., Sharma, A., Namsrai, B., Tran, N., Finger, E. B., Garwood, M., Haynes, C. L., & Bischof, J. C. (2020) · Advanced Science · doi:10.1002/advs.201901624 · Cited by 101

Antiadhesion Organogel Materials: From Liquid to Solid

Lv, J., Yao, X., Zheng, Y., Wang, J., & Jiang, L. (2017) · Advanced Materials · doi:10.1002/adma.201703032 · Cited by 99

Bioinspired l ‑Proline Oligomers for the Cryopreservation of Oocytes via Controlling Ice Growth

Qin, Q., Zhao, L., Liu, Z., Liu, T., Qu, J., Zhang, X., Li, R., Yan, L., Yan, J., Jin, S., et al. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c02719 · Cited by 97

Dual Suppression Effect of Magnetic Induction Heating and Microencapsulation on Ice Crystallization Enables Low-Cryoprotectant Vitrification of Stem Cell–Alginate Hydrogel Constructs

Liu, X., Zhao, G., Chen, Z., Panhwar, F., & He, X. (2018) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.8b04496 · Cited by 94

Anti-Freezing multiphase gel materials: Bioinspired design strategies and applications

Xu, Y., Rong, Q., Zhao, T., & Liu, M. (2020) · Giant · doi:10.1016/j.giant.2020.100014 · Cited by 93

A Comprehensive Evaluation of the Effects and Mechanisms of Antifreeze Proteins during Low-Temperature Preservation

Wang, J. (2000) · Cryobiology · doi:10.1006/cryo.2000.2265 · Cited by 92

Contributions of unfrozen fraction and of salt concentration to the survival of slowly frozen human erythrocytes: Influence of warming rate

Mazur, P., & Rigopoulos, N. (1983) · Cryobiology · doi:10.1016/0011-2240(83)90016-0 · Cited by 92

Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage

Mitchell, D. E., Fayter, A. E. R., Deller, R. C., Hasan, M., Gutierrez‐Marcos, J., & Gibson, M. I. (2018) · Materials Horizons · doi:10.1039/c8mh00727f · Cited by 91

Cold-Responsive Nanoparticle Enables Intracellular Delivery and Rapid Release of Trehalose for Organic-Solvent-Free Cryopreservation

Zhang, Y., Wang, H., Stewart, S., Jiang, B., Ou, W., Zhao, G., & He, X. (2019) · Nano Letters · doi:10.1021/acs.nanolett.9b04109 · Cited by 91

Small molecule ice recrystallization inhibitors mitigate red blood cell lysis during freezing, transient warming and thawing

Briard, J. G., Poisson, J. S., Turner, T. R., Capicciotti, C. J., Acker, J. P., & Ben, R. N. (2016) · Scientific Reports · doi:10.1038/srep23619 · Cited by 89

Cell armor for protection against environmental stress: Advances, challenges and applications in micro- and nanoencapsulation of mammalian cells

Hasturk, O., & Kaplan, D. L. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.11.040 · Cited by 86

Dimethyl sulfoxide-free cryopreservation for cell therapy: A review

Weng, L., & Beauchesne, P. R. (2020) · Cryobiology · doi:10.1016/j.cryobiol.2020.03.012 · Cited by 85

Ice Nucleation Properties of Oxidized Carbon Nanomaterials

Whale, T. F., Rosillo‐Lopez, M., Murray, B. J., & Salzmann, C. G. (2015) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.5b01096 · Cited by 84

Predehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation

Huang, H., Zhao, G., Zhang, Y., Xu, J., Toth, T. L., & He, X. (2017) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.7b00201 · Cited by 81

Apatite nanoparticles strongly improve red blood cell cryopreservation by mediating trehalose delivery via enhanced membrane permeation

Stefanic, M., Ward, K. R., Tawfik, H., Seemann, R., Baulin, V. A., Guo, Y., Fleury, J., & Drouet, C. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.06.018 · Cited by 79

Tuning Ice Nucleation with Supercharged Polypeptides

Yang, H., Ma, C., Li, K., et al. (2016) · Advanced Materials · doi:10.1002/adma.201600496 · Cited by 78

Characterization of Laser Gold Nanowarming: A Platform for Millimeter-Scale Cryopreservation

Khosla, K., Zhan, L., Bhati, A., Carley-Clopton, A., Hagedorn, M., & Bischof, J. C. (2018) · Langmuir · doi:10.1021/acs.langmuir.8b03011 · Cited by 73

Preferential vitrification of water in small alginate microcapsules significantly augments cell cryopreservation by vitrification

Zhang, W., Yang, G., Zhang, A., et al. (2009) · Biomedical Microdevices · doi:10.1007/s10544-009-9363-z · Cited by 72

An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells

Baharvand, H., Salekdeh, G. H., Taei, A., & Mollamohammadi, S. (2010) · Nature Protocols · doi:10.1038/nprot.2009.247 · Cited by 72

Cold‐Responsive Nanocapsules Enable the Sole‐Cryoprotectant‐Trehalose Cryopreservation of β Cell–Laden Hydrogels for Diabetes Treatment

Cheng, Y., Yu, Y., Zhang, Y., Zhao, G., & Zhao, Y. (2019) · Small · doi:10.1002/smll.201904290 · Cited by 69

Soft liquid metal nanoparticles achieve reduced crystal nucleation and ultrarapid rewarming for human bone marrow stromal cell and blood vessel cryopreservation

Hou, Y., Lu, C., Dou, M., Zhang, C., Chang, H., Liu, J., & Rao, W. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.11.023 · Cited by 69

Challenges in Umbilical Cord Blood Stem Cell Banking for Stem Cell Reviews and Reports

Ballen, K. K. (2009) · Stem Cell Reviews and Reports · doi:10.1007/s12015-009-9105-x · Cited by 64

Novel Microwave Technology for Cryopreservation of Biomaterials by Suppression of Apparent Ice Formation

Jackson, T. H., Ungan, A., Critser, J. K., & Gao, D. Y. (1997) · Cryobiology · doi:10.1006/cryo.1997.2016 · Cited by 63

Fluorinated graphene provides long lasting ice inhibition in high humidity

Akhtar, N., Anemone, G., Farías, D., & Holst, B. (2018) · Carbon · doi:10.1016/j.carbon.2018.09.008 · Cited by 62

DSC Measurement of Cell Suspensions during Successive Freezing Runs: Implications for the Mechanisms of Intracellular Ice Formation

Bryant, G. (1995) · Cryobiology · doi:10.1006/cryo.1995.1011 · Cited by 59

Quantitative study on the antifreeze protein mimetic ice growth inhibition properties of poly(ampholytes) derived from vinyl-based polymers

Mitchell, D. E., Lilliman, M., Spain, S. G., & Gibson, M. I. (2014) · Biomaterials Science · doi:10.1039/c4bm00153b · Cited by 54

Centrifugal microfluidics for ultra-rapid fabrication of versatile hydrogel microcarriers

Cheng, Y., Zhang, X., Cao, Y., Tian, C., Li, Y., Wang, M., Zhao, Y., & Zhao, G. (2018) · Applied Materials Today · doi:10.1016/j.apmt.2018.08.012 · Cited by 54

Nanoparticle‐Mediated Intracellular Protection of Natural Killer Cells Avoids Cryoinjury and Retains Potent Antitumor Functions

Yao, X., Jovevski, J. J., Todd, M. F., Xu, R. F., Li, Y., Wang, J., & Matosevic, S. (2020) · Advanced Science · doi:10.1002/advs.201902938 · Cited by 54

Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers

Deller, R. C., Pessin, J. E., Vatish, M., Mitchell, D. A., & Gibson, M. I. (2016) · Biomaterials Science · doi:10.1039/c6bm00129g · Cited by 53

Attempted canine renal cryopreservation using dimethyl sulphoxide helium perfusion and microwave thawing

Pegg, D. E., Green, C. J., & Walter, C. A. (1978) · Cryobiology · doi:10.1016/0011-2240(78)90086-x · Cited by 52

Effects of ultrasound-assisted thawing on the quality of edamames [Glycine max (L.) Merrill] frozen using different freezing methods

Cheng, X., Zhang, M., & Adhikari, B. (2014) · Food Science and Biotechnology · doi:10.1007/s10068-014-0150-0 · Cited by 51

Bioinspired Preservation of Natural Killer Cells for Cancer Immunotherapy

Assal, R. E., Abou‐Elkacem, L., Tocchio, A., Pasley, S., Matosevic, S., Kaplan, D. L., Zylberberg, C., & Demirci, U. (2019) · Advanced Science · doi:10.1002/advs.201802045 · Cited by 48

A Serendipitous Discovery of Antifreeze Protein-Specific Activity in C-Linked Antifreeze Glycoprotein Analogs

Eniade, A., Madhusudhan, P., Ben, R. N., Wang, J. B., & Horwath, K. L. (2003) · Cell Biochemistry and Biophysics · doi:10.1385/cbb:38:2:115 · Cited by 47

From Nanowarming to Thermoregulation: New Multiscale Applications of Bioheat Transfer

Bischof, J. C., & Diller, K. R. (2018) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071516-044532 · Cited by 46

Inhibition of Heterogeneous Ice Nucleation by Bioinspired Coatings of Polyampholytes

He, Z., Zheng, L., Liu, Z., Jin, S., Li, C., & Wang, J. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b10014 · Cited by 45

Effect of Antifreeze Protein on Mouse Ovarian Tissue Cryopreservation and Transplantation

Lee, J. R., Youm, H. W., Lee, H. J., Jee, B. C., Suh, C. S., & Kim, S. H. (2015) · Yonsei Medical Journal · doi:10.3349/ymj.2015.56.3.778 · Cited by 44

Near-infrared laser mediated modulation of ice crystallization by two-dimensional nanosheets enables high-survival recovery of biological cells from cryogenic temperatures

Panhwar, F., Chen, Z., Hossain, S. M. C., Wang, M., Haider, Z., Memon, K., Chen, P., & Zhao, G. (2018) · Nanoscale · doi:10.1039/c8nr01349g · Cited by 44

Multivalent Presentation of Ice Recrystallization Inhibiting Polymers on Nanoparticles Retains Activity

Stubbs, C., Wilkins, L. E., Fayter, A. E. R., Walker, M., & Gibson, M. I. (2018) · Langmuir · doi:10.1021/acs.langmuir.8b01952 · Cited by 41

Designing the next generation of cryoprotectants – From proteins to small molecules

Ampaw, A. A., Charlton, T. A., Briard, J. G., & Ben, R. N. (2018) · Peptide Science · doi:10.1002/pep2.24086 · Cited by 41

Ice Nucleation in Emulsified Aqueous Solutions of Antifreeze Protein Type III and Poly(vinyl alcohol)

Inada, T., Koyama, T., Goto, F., & Seto, T. (2011) · The Journal of Physical Chemistry B · doi:10.1021/jp111745v · Cited by 40

Synthesis of Highly Biocompatible and Temperature-Responsive Physical Gels for Cryopreservation and 3D Cell Culture

Nagao, M., Sengupta, J., Diaz‐Dussan, D., Adam, M. K., Wu, M. Y., Acker, J. P., Ben, R. N., Ishihara, K., Zeng, H., Miura, Y., et al. (2018) · ACS Applied Bio Materials · doi:10.1021/acsabm.8b00096 · Cited by 38

The Unusual Properties of Polytetrafluoroethylene Enable Massive‐Volume Vitrification of Stem Cells with Low‐Concentration Cryoprotectants

Cao, Y., Zhao, G., Panhwar, F., Zhang, X., Chen, Z., Cheng, L., Zang, C., Liu, F., Zhao, Y., & He, X. (2018) · Advanced Materials Technologies · doi:10.1002/admt.201800289 · Cited by 37

Amphipathic polymer-mediated uptake of trehalose for dimethyl sulfoxide-free human cell cryopreservation

Sharp, D. M. C., Picken, A., Morris, T. J., Hewitt, C. J., Coopman, K., & Slater, N. K. H. (2013) · Cryobiology · doi:10.1016/j.cryobiol.2013.09.002 · Cited by 36

Cryopreservation of human embryonic stem cells by a programmed freezer with an oscillating magnetic field

Lin, P.-Y., Yang, Y.-C., Hung, S., Lee, S., Lee, M., Chu, I., & Hwang, S. (2013) · Cryobiology · doi:10.1016/j.cryobiol.2013.02.061 · Cited by 35

Magnetothermal heating facilitates the cryogenic recovery of stem cell–laden alginate–Fe 3 O 4 nanocomposite hydrogels

Zhang, X., Zhao, G., Cao, Y., Haider, Z., Wang, M., & Fu, J. (2018) · Biomaterials Science · doi:10.1039/c8bm01004h · Cited by 35

Mechanisms of cryoinjury in organs

Pegg, D. E. (1981) · Cryobiology · doi:10.1016/0011-2240(81)90149-8 · Cited by 33

Cryopreservation of rat MSCs by use of a programmed freezer with magnetic field

Kojima, S., Kaku, M., Kawata, T., Sumi, H., Shikata, H., Abonti, T. R., Kojima, S., Fujita, T., Motokawa, M., & Tanne, K. (2013) · Cryobiology · doi:10.1016/j.cryobiol.2013.08.003 · Cited by 31

Cryopreservation and low temperature biology in blood transfusion

(1990) · Springer eBooks · doi:10.1007/978-1-4613-1515-5 · Cited by 31

Tuning Ice Nucleation and Propagation with Counterions on Multilayer Hydrogels

Guo, Q., He, Z., Jin, Y., Zhang, S., Wu, S., Bai, G., Han, X. M., Liu, Z., Jin, S., Zhao, L., et al. (2018) · Langmuir · doi:10.1021/acs.langmuir.8b02106 · Cited by 26

Decoration of Chondroitin Polysaccharide with Threonine: Synthesis, Conformational Study, and Ice-Recrystallization Inhibition Activity

Laezza, A., Casillo, A., Cosconati, S., Biggs, C. I., Fabozzi, A., Paduano, L., Iadonisi, A., Novellino, E., Gibson, M. I., Randazzo, A., et al. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.7b00326 · Cited by 20

Molecular dynamics study on the role of solvation water in the adsorption of hyperactive AFP to the ice surface

Grabowska, J., Kuffel, A., & Zielkiewicz, J. (2018) · Physical Chemistry Chemical Physics · doi:10.1039/c8cp05027a · Cited by 16

Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

Aziz, J., Morris, G., Rizk, M., Shorr, R., Mercer, D., Young, K., & Allan, D. (2017) · Transfusion · doi:10.1111/trf.14360 · Cited by 10

Cell-encapsulating droplet formation and freezing

Youn, J. R., & Song, Y. S. (2012) · Applied Physics Letters · doi:10.1063/1.4754611 · Cited by 9

Preparation, characterization and freeze-drying protective mechanisms of natural polysaccharides as emerging lyoprotectants: A review

Zhang, M., Hao, L., Wang, Z., Yang, H., Zhou, R., Jiang, G., & Wu, C. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.154838