Butyrate enhances intestinal epithelial barrier function via up-regulation of tight junction protein claudin-1 transcription
Abstract
Background Barrier function is essential for the maintenance of normal intestinal function. Dysregulation of the intestinal barrier underlies a wide range of disorders.Aim Previously, we found that sodium butyrate (NaB) decreased the molecular permeability of intestinal barrier in vivo model, but the mechanism by which NaB facilitated the tightness of tight junctions (TJs) in small intestinal epithelium needed further studies.Methods In vitro culture of the cdx2-IEC monolayer was used to mimic barrier function. The TJs were assessed by transepithelial electrical resistance (TEER) and paracellular flux of fluorescein isothiocyanate-conjugated dextran 40,000 (FD-40), Western blot, Q-RT-PCR, and immunofluorescence. Promoter and chromatin immunoprecipitation (ChIP) assays were also done to analyze the Claudin-1 gene.Results NaB decreased FD-40 flux, increased TEER and TJ protein Claudin-1 expression, induced ZO-1 and Occludin redistribution in cellular membrane, and reversed the damage effect after calcium (Ca(2+)) switch assay. Silencing Claudin-1 prevented protective function of NaB from enhancing intestinal barrier integrity. Further studies demonstrated that NaB increased Claudin-1 transcription by facilitating the interaction between transcription factor SP1 and a specific motif within the promoter region of Claudin-1. This SP1 binding motif was located upstream of the coding region (-138 to -76 bp) and indispensable for the transcription of Claudin-1 following NaB treatment. ChIP assay confirmed the association between SP1 and Claudin-1 promoter, and the elimination of the SP1 binding site by point mutation resulted in a significant loss of Claudin-1 transcription after NaB dealing.Conclusions NaB enhanced intestinal barrier function through increasing Claudin-1 transcription via facilitating the association between SP1 and Claudin-1 promoter.
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Treatment with sodium butyrate led to the upregulation of CLDN-1 transcription by increasing the interaction between the motif specific promoter region of CLDN-1 and transcription factor SP1.
“Treatment with sodium butyrate led to the upregulation of CLDN-1 transcription by increasing the interaction between the motif specific promoter region of CLDN-1 and transcription factor SP1.82”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [82]
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- Authors
- Wang, H.-B., Wang, P.-Y., Wang, X., Wan, Y.-L., & Liu, Y.-C.
- Affiliations
- Peking University (CN)
- Corresponding author
- Wang, Peng-Yuan
- Venue
- Digestive Diseases and Sciences
- Published
- 2012-06-09
- Volume
- 57
- Issue
- 12
- Pages
- 3126-3135
- ISSN
- 0163-2116 (ISSN-L)
- 1573-2568
- OpenAlex
- W2029180586
- Cited by
- 827
- References
- 23
- Citations by year
- 2026: 91 · 2025: 118 · 2024: 81 · 2023: 92 · 2022: 111 · 2021: 89 · 2020: 73 · 2019: 51 · 2018: 39 · 2017: 26 · 2016: 21 · 2015: 18 · 2014: 11 · 2013: 6
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Digestive Diseases and Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- sodium butyrate, intestinal barrier function, claudin-1, tight junctions, Sp1, transcriptional regulation, ZO-1, occludin, small intestinal epithelium, transepithelial electrical resistance
- MeSH terms
- Amino Acid Sequence, Animals, Butyrates, Cell Line, Intestinal Mucosa, Promoter Regions, Genetic, Protein Binding, Transcription, Genetic, Up-Regulation, Sp1 Transcription Factor, Amino Acid Motifs, RNA Interference, Chromatin Immunoprecipitation, Rats, Claudin-1
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