Pyrrolopyrrole aza-BODIPY-based NIR-II fluorophores for in vivo dynamic vascular dysfunction visualization of vascular-targeted photodynamic therapy
Open accessAbstract
Real-time monitoring vascular responses is crucial for evaluating the therapeutic effects of vascular-targeted photodynamic therapy (V-PDT). Herein, we developed a highly-stable and bright aggregation induced emission (AIE) fluorophore (PTPE3 NP) for dynamic fluorescence (FL) imaging of vascular dysfunction beyond 1300 nm window during V-PDT. The superior brightness (ϵ max Φ f>1000 nm ≈ 180.05 M-1 cm-1) and high resolution of PTPE3 NP affords not only high-clarity images of whole-body and local vasculature (hindlimbs, mesentery, and tumor) but also high-speed video imaging for tracking blood circulation process. By virtue of the NPs' prolonged blood circulation time (t 1/2 ≈ 86.5 min) and excellent photo/chemical (pH, RONS) stability, mesenteric and tumor vascular dysfunction (thrombosis formation, vessel occlusion, and hemorrhage) can be successfully visualized during V-PDT by FL imaging for the first time. Furthermore, the reduction of blood flow velocity (BFV) can be monitored in real time for precisely evaluating efficacy of V-PDT. These provide a powerful approach for assessing vascular responses during V-PDT and promote the development of advanced fluorophores for biological imaging.
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Advances on fluorescence bioimaging and photo-mediated therapy of NIR-II small molecule dyes
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- Authors
- Bian, S., Zheng, X., Liu, W., Li, J., Gao, Z., Ren, H., Zhang, W., Lee, C.-S., & Wang, P.
- Affiliations
- Chinese Academy of Sciences (CN)
- City University of Hong Kong (HK)
- Corresponding author
- Zheng, Xiuli
- Lee, Chun-Sing
- Wang, Pengfei
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2023-07
- Volume
- 298
- Pages
- 122130
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W4367056759
- Cited by
- 29
- References
- 52
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 9 · 2023: 1
- FWCI
- 2.13
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- CityU Scholars (submittedVersion, cc-by-nc-nd)
- Funders
- National Natural Science Foundation of China (21873110, 61720106014, 62005294)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- NIR-II fluorescence imaging, aggregation-induced emission, vascular-targeted photodynamic therapy, vascular dysfunction, thrombosis, blood flow velocity, tumor vasculature, mesenteric vasculature, blood circulation time
- MeSH terms
- Animals, Boron Compounds, Humans, Neoplasms, Photochemotherapy, Optical Imaging
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