Superoxide flashes in single mitochondria
Open accessAbstract
In quiescent cells, mitochondria are the primary source of reactive oxygen species (ROS), which are generated by leakiness of the electron transport chain (ETC). High levels of ROS can trigger cell death, whereas lower levels drive diverse and important cellular functions. We show here by employing a newly developed mitochondrial matrix-targeted superoxide indicator, that individual mitochondria undergo spontaneous bursts of superoxide generation, termed "superoxide flashes." Superoxide flashes occur randomly in space and time, exhibit all-or-none properties, and provide a vital source of superoxide production across many different cell types. Individual flashes are triggered by transient openings of the mitochondrial permeability transition pore stimulating superoxide production by the ETC. Furthermore, we observe a flurry of superoxide flash activity during reoxygenation of cardiomyocytes after hypoxia, which is inhibited by the cardioprotective compound adenosine. We propose that superoxide flashes could serve as a valuable biomarker for a wide variety of oxidative stress-related diseases.
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Alterations in mPTP function contribute to the aging-related loss of muscle mass and function.
“Alterations in mPTP function are undoubtedly contributing to the aging-related loss of muscle mass and function, since mPTP opening can trigger several atrophy-regulating pathways.”
From Mitochondrial Dynamics and Mitophagy in Skeletal Muscle Health and Aging (2021) · cites this paper as [15]
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- Authors
- Wang, W., Fang, H., Groom, L., Cheng, A., Zhang, W., Liu, J., Wang, X., Li, K., Han, P., Zheng, M., Yin, J., Wang, W., Mattson, M. P., Kao, J. P. Y., Lakatta, E. G., Sheu, S.-S., Ouyang, K., Chen, J., Dirksen, R. T., & Cheng, H.
- Affiliations
- National Institutes of Health (US)
- Peking University (CN)
- University of Rochester Medicine (US)
- University of Maryland, Baltimore (US)
- University of California San Diego (US)
- Corresponding author
- Wang, Wang
- Cheng, Heping
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 134
- Issue
- 2
- Pages
- 279-290
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC2547996
- OpenAlex
- W1993039541
- Cited by
- 715
- References
- 58
- Citations by year
- 2026: 11 · 2025: 20 · 2024: 24 · 2023: 12 · 2022: 23 · 2021: 34 · 2020: 36 · 2019: 44 · 2018: 43 · 2017: 45 · 2016: 52 · 2015: 57 · 2014: 60 · 2013: 50 · 2012: 60
- FWCI
- 19.77
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1878 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (HL 33333, R01-HL-093671, R01HL033333)
- National Institute of General Medical Sciences (R01GM056481)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R29AR044657, AR-44657, R01AR044657)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- mitochondrial superoxide, reactive oxygen species, mitochondrial permeability transition pore, mitochondrial matrix, hypoxia/reoxygenation, cardiomyocytes, adenosine, oxidative stress biomarkers, cell death
- MeSH terms
- Adenoviridae, Animals, Cells, Cultured, Humans, Luminescent Proteins, Mitochondria, Neurons, Superoxides, Cell Hypoxia, Reactive Oxygen Species, Oxidative Stress, Muscle Cells, Myocytes, Cardiac, Cell Line, Tumor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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