The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
In Vitro and in Vivo RNA Inhibition by CD9-HuR Functionalized Exosomes Encapsulated with miRNA or CRISPR/dCas9
Abstract
In vitro and in vivo delivery of RNAs of interest holds promise for gene therapy. Recently, exosomes are considered as a kind of rational vehicle for RNA delivery, especially miRNA and/or siRNA, while the loading efficiency is limited. In this study, we engineered the exosomes for RNA loading by constructing a fusion protein in which the exosomal membrane protein CD9 was fused with RNA binding protein, while the RNA of interest either natively harbors or is engineered to have the elements for the binding. By proof-of-principle experiments, we here fused CD9 with HuR, an RNA binding protein interacting with miR-155 with a relatively high affinity. In the exosome packaging cells, the fused CD9-HuR successfully enriched miR-155 into exosomes when miR-155 was excessively expressed. Moreover, miR-155 encapsulated in the exosomes in turn could be efficiently delivered into the recipient cells and recognized the endogenous targets. In addition, we also revealed that the CD9-HuR exosomes could enrich the functional miRNA inhibitor or CRISPR/dCas9 when the RNAs were engineered to have the AU rich elements. Taken together, we here have established a novel strategy for enhanced RNA cargo encapsulation into engineered exosomes, which in turn functions in the recipient cells.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In exosome-producing cells, the CD9-HuR fusion protein effectively enriched exosomes with miR-155 when miR-155 was overexpressed.”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [105]
In the citing paper
“Moreover, miR-155 encapsulated in these exosomes can be efficiently delivered to recipient cells and target endogenous molecules (Fig. 2C) [105].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [105]
Citations
Cites
miRBase: annotating high confidence microRNAs using deep sequencing data
Kozomara, A., & Griffiths-Jones, S. (2014) · Nucleic Acids Research · doi:10.1093/nar/gkt1181 · Cited by 5,073
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Extracellular vesicles: biology and emerging therapeutic opportunities
EL Andaloussi, S., Mäger, I., Breakefield, X. O., & Wood, M. J. A. (2013) · Nature Reviews Drug Discovery · doi:10.1038/nrd3978 · Cited by 3,571
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825
Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function
Zhang, J., Li, S., Li, L., Li, M., Guo, C., Yao, J., & Mi, S. (2015) · Genomics, Proteomics & Bioinformatics · doi:10.1016/j.gpb.2015.02.001 · Cited by 1,901
Tian, Y., Li, S., Song, J., Ji, T., Zhu, M., Anderson, G. J., Wei, J., & Nie, G. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.083 · Cited by 1,841
Ambros, V. (2001) · Cell · doi:10.1016/s0092-8674(01)00616-x · Cited by 1,705
Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells
Ohno, S.-I., Takanashi, M., Sudo, K., Ueda, S., Ishikawa, A., Matsuyama, N., Fujita, K., Mizutani, T., Ohgi, T., Ochiya, T., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2012.180 · Cited by 1,666
LncRNA: A link between RNA and cancer
Yang, G., Lu, X., & Yuan, L. (2014) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2014.08.012 · Cited by 992
Self-citationLu, L., Thai, T., Calado, D. P., Chaudhry, A., Kubo, M., Tanaka, K., Loeb, G. B., Lee, H., Yoshimura, A., Rajewsky, K., et al. (2009) · Immunity · doi:10.1016/j.immuni.2008.11.010 · Cited by 797
Flagged first, then by citations
Cited by
Sorted by citations
Zhang, Y., Bi, J., Huang, J., Tang, Y., Du, S., & Li, P. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s264498 · Cited by 1,323
CRISPR/Cas9 therapeutics: progress and prospects
Li, T., Yang, Y., Qi, H., Cui, W., Zhang, L., Fu, X., He, X., Liu, M., Li, P., & Yu, T. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01309-7 · Cited by 824
Meng, W., He, C., Hao, Y., Wang, L., Li, L., & Zhu, G. (2020) · Drug Delivery · doi:10.1080/10717544.2020.1748758 · Cited by 592
Salunkhe, S. A., Dheeraj, Basak, M., Chitkara, D., & Mittal, A. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.07.042 · Cited by 513
Engineered exosomes from different sources for cancer-targeted therapy
Zhang, M. H., Hu, S., Liu, L., Dang, P., Liu, Y., Sun, Z., Qiao, B., & Wang, C. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01382-y · Cited by 493
Exosome engineering: Current progress in cargo loading and targeted delivery
Fu, S., Wang, Y., Xia, X., & Zheng, J. C. (2020) · NanoImpact · doi:10.1016/j.impact.2020.100261 · Cited by 444
Engineered Extracellular Vesicles for Cancer Therapy
Zhang, X., Zhang, H., Gu, J., Zhang, J., Shi, H., Qian, H., Wang, D., Xu, W., Pan, J., & Santos, H. A. (2021) · Advanced Materials · doi:10.1002/adma.202005709 · Cited by 428
Regulation of cargo selection in exosome biogenesis and its biomedical applications in cancer
Lee, Y. J., Shin, K. J., & Chae, Y. C. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01209-y · Cited by 427
Therapeutic roles of mesenchymal stem cell-derived extracellular vesicles in cancer
Weng, Z., Zhang, B., Wu, C., Yu, F., Han, B. R., Li, B., & Li, L. (2021) · Journal of Hematology & Oncology · doi:10.1186/s13045-021-01141-y · Cited by 420
Liang, Y., Xu, X., Li, X., Xiong, J., Li, B., Duan, L., Wang, D., & Xia, J. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c10458 · Cited by 391
Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing
Duan, L., Ouyang, K., Xu, X., Xu, L., Wen, C., Zhou, X., Qin, Z., Xu, Z., Sun, W., & Liang, Y. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.673286 · Cited by 377
Self-citationExtracellular vesicles: A bright star of nanomedicine
Wu, P., Zhang, B., Ocansey, D. K. W., Xu, W., & Qian, H. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120467 · Cited by 368
Exosome engineering in cell therapy and drug delivery
Sadeghi, S., Tehrani, F. R., Tahmasebi, S., Shafiee, A., & Hashemi, S. M. (2023) · Inflammopharmacology · doi:10.1007/s10787-022-01115-7 · Cited by 351
Exosomes, a New Star for Targeted Delivery
Chen, H., Wang, L., Zeng, X., Schwarz, H., Nanda, H. S., Peng, X., & Zhou, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.751079 · Cited by 349
Non-viral vectors for RNA delivery
Yan, Y., Liu, X.-Y., Lu, A., Wang, X.-Y., Jiang, L.-X., & Wang, J.-C. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.01.008 · Cited by 332
Recent advances in exosome-mediated nucleic acid delivery for cancer therapy
Zhang, Y., Liu, Q., Zhang, X., Huang, H., Tang, S., Chai, Y., Xu, Z., Li, M., Chen, X., Liu, J., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01472-z · Cited by 306
Zhu, Q., Ling, X., Yang, Y., Zhang, J., Li, Q., Niu, X., Hu, G., Chen, B., Li, H., Wang, Y., et al. (2019) · Advanced Science · doi:10.1002/advs.201801899 · Cited by 306
Song, H., Li, X., Zhao, Z., Jin, Q., Wang, Y., Cui, J., Weng, W., Cao, L., Chen, X., Hu, Y., et al. (2019) · Nano Letters · doi:10.1021/acs.nanolett.9b00287 · Cited by 304
Wan, T. Y., Zhong, J., Pan, Q., Zhou, T., Yuan, P., & Liu, X. (2022) · Science Advances · doi:10.1126/sciadv.abp9435 · Cited by 314
Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading
Rädler, J., Gupta, D., Zickler, A. M., & Andaloussi, S. E. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.02.013 · Cited by 298
The clinical potential of circulating microRNAs in obesity
Ji, C., & Guo, X. (2019) · Nature Reviews Endocrinology · doi:10.1038/s41574-019-0260-0 · Cited by 263
Huda, M. N., Nafiujjaman, M., Deaguero, I. G., Okonkwo, J., Hill, M. L., Kim, T. H., & Nurunnabi, M. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00217 · Cited by 268
Designer Exosomes: A New Platform for Biotechnology Therapeutics
Jafari, D., Shajari, S., Jafari, R., Mardi, N., Gomari, H., Ganji, F., Moghadam, M. F., & Samadikuchaksaraei, A. (2020) · BioDrugs · doi:10.1007/s40259-020-00434-x · Cited by 255
The tremendous biomedical potential of bacterial extracellular vesicles
Xie, J., Li, Q., Haesebrouck, F., Hoecke, L. V., & Vandenbroucke, R. E. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.03.005 · Cited by 245
Exosome-mediated delivery of gene vectors for gene therapy
Duan, L., Xu, L., Xu, X., Qin, Z., Zhou, X., Xiao, Y., Liang, Y., & Xia, J. (2021) · Nanoscale · doi:10.1039/d0nr07622h · Cited by 238
Self-citationDu, J., Wan, Z., Wang, C., Fan, L., Wei, M., Wang, D., & Hao, Q. (2021) · Theranostics · doi:10.7150/thno.59121 · Cited by 235
Self-citationChondrocyte-specific genomic editing enabled by hybrid exosomes for osteoarthritis treatment
Liang, Y., Xu, X., Xu, L., Iqbal, Z., Ouyang, K., Zhang, H., Wen, C., Duan, L., & Xia, J. (2022) · Theranostics · doi:10.7150/thno.69368 · Cited by 217
Extracellular vesicles as a drug delivery system: A systematic review of preclinical studies
de Castilla, P. E. M., Tong, L., Huang, C., Sofias, A. M., Pastorin, G., Chen, X. S., Storm, G., Schiffelers, R. M., & Wang, J. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.05.011 · Cited by 219
Overview and Update on Methods for Cargo Loading into Extracellular Vesicles
Han, Y., Jones, T. W., Dutta, S., Zhu, Y., Wang, X., Narayanan, S. P., Fagan, S. C., & Zhang, D. (2021) · Processes · doi:10.3390/pr9020356 · Cited by 218
da Silva, A. M., Lázaro‐Ibáñez, E., Gunnarsson, A., Dhande, A., Daaboul, G. G., Peacock, B., Osteikoetxea, X., Salmond, N., Friis, K. P., Shatnyeva, O., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12130 · Cited by 195
Rational designs of in vivo CRISPR-Cas delivery systems
Xu, C., Chen, G., Luo, Y., Zhang, Y., Zhao, G., Lu, Z.-D., Czarna, A., Gu, Z., & Wang, J. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.11.005 · Cited by 187
Exosomes in atherosclerosis: performers, bystanders, biomarkers, and therapeutic targets
Wang, C., Li, Z., Liu, Y., & Yuan, L. (2021) · Theranostics · doi:10.7150/thno.56035 · Cited by 194
Self-citationThe role of Exosomal miRNAs in cancer
Li, C., Zhou, T., Chen, J., Li, R., Chen, H., Luo, S., Chen, D., Cai, C., & Li, W. (2022) · Journal of Translational Medicine · doi:10.1186/s12967-021-03215-4 · Cited by 181
Ye, Y., Zhang, X., Xie, F., Xu, B., Xie, P., Yang, T., Shi, Q., Zhang, C., Zhang, Y., Chen, J., et al. (2020) · Biomaterials Science · doi:10.1039/d0bm00427h · Cited by 184
Chemically Modified Platforms for Better RNA Therapeutics
Shi, Y., Zhen, X., Zhang, Y., Li, Y., Koo, S., Saiding, Q., Kong, N., Liu, G., Chen, W., & Tao, W. (2024) · Chemical Reviews · doi:10.1021/acs.chemrev.3c00611 · Cited by 189
Yin, C., Han, Q., Xu, D., Zheng, B., Zhao, X., & Zhang, J. (2019) · OncoImmunology · doi:10.1080/2162402x.2019.1601479 · Cited by 182
Advancements and challenges in developing in vivo CAR T cell therapies for cancer treatment
Bui, T. A., Mei, H., Sang, R., Gallego‐Ortega, D., & Deng, W. (2024) · EBioMedicine · doi:10.1016/j.ebiom.2024.105266 · Cited by 190
Mi, B., Chen, L., Xiong, Y., Yang, Y., Panayi, A. C., Xue, H., Hu, Y., Yan, C., Hu, L., Xie, X., et al. (2022) · ACS Nano · doi:10.1021/acsnano.1c08284 · Cited by 175
Liang, Y., Iqbal, Z., Lu, J., Wang, J., Zhang, H., Chen, X., Duan, L., & Xia, J. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2022.10.008 · Cited by 162
Brown adipose tissue-derived exosomes mitigate the metabolic syndrome in high fat diet mice
Zhou, X., Li, Z., Qi, M., Zhao, P., Duan, Y., Yang, G., & Yuan, L. (2020) · Theranostics · doi:10.7150/thno.43968 · Cited by 160
Self-citationYang, J., Wu, S., Hou, L., Zhu, D., Yin, S., Yang, G., & Wang, Y. (2020) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2020.06.013 · Cited by 154
Self-citationHan, X., Alu, A., Liu, H., Shi, Y., Wei, X., Cai, L., & Wei, Y. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.01.011 · Cited by 165
The Role of Exosomal microRNAs and Oxidative Stress in Neurodegenerative Diseases
Wang, X., Zhou, Y., Gao, Q., Ping, D., Wang, Y., Wu, W., Xu, L., Fang, Y., Zhang, J., & Shao, A. (2020) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2020/3232869 · Cited by 151
Exosome for mRNA delivery: Strategies and therapeutic applications
Iqbal, Z., Rehman, K., Mahmood, A., Shabbir, M., Liang, Y., Duan, L., & Zeng, H. (2024) · Journal of Nanobiotechnology · doi:10.1186/s12951-024-02634-x · Cited by 144
Extracellular vesicles: Emerging tools as therapeutic agent carriers
Liu, S., Wu, X., Chandra, S., Lyon, C. J., Ning, B., Jiang, L., Fan, J., & Hu, Y. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.05.002 · Cited by 149
Extracellular vesicle-based Nanotherapeutics: Emerging frontiers in anti-inflammatory therapy
Tang, T., Wang, B., Lv, L., & Liu, B. (2020) · Theranostics · doi:10.7150/thno.47865 · Cited by 137
Bu, T., Li, Z., Hou, Y., Sun, W., Zhang, R., Zhao, L., Wei, M., Yang, G., & Yuan, L. (2021) · Theranostics · doi:10.7150/thno.64229 · Cited by 134
Self-citationEngineering therapeutical extracellular vesicles for clinical translation
Ma, Y., Dong, S., Grippin, A., Teng, L., Lee, A. S., Kim, B. Y. S., & Jiang, W. (2024) · Trends in biotechnology · doi:10.1016/j.tibtech.2024.08.007 · Cited by 136
CRISPR/Cas9: Principle, Applications, and Delivery through Extracellular Vesicles
Horodecka, K., & Düchler, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22116072 · Cited by 121
Barman, B., Sung, B. H., Krystofiak, E., Ping, J., Ramirez, M. A., Millis, B. A., Allen, R. M., Prasad, N., Chetyrkin, S. V., Calcutt, M. W., et al. (2022) · Developmental Cell · doi:10.1016/j.devcel.2022.03.012 · Cited by 126
Gangadaran, P., & Ahn, B. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12050442 · Cited by 119
Exosomes as drug delivery vehicle and contributor of resistance to anticancer drugs
Chinnappan, M., Srivastava, A., Amreddy, N., Razaq, M., Pareek, V., Ahmed, R. A., Mehta, M., Peterson, J. E. G., Munshi, A., & Ramesh, R. (2020) · Cancer Letters · doi:10.1016/j.canlet.2020.05.004 · Cited by 119
Nanomedicine to target multidrug resistant tumors
Lepeltier, É., Rijo, P. M., Rizzolio, F., Popovtzer, R., Petrikaitė, V., Assaraf, Y. G., & Passirani, C. (2020) · Drug Resistance Updates · doi:10.1016/j.drup.2020.100704 · Cited by 116
The cancer exosomes: Clinical implications, applications and challenges
Tang, Z., Li, D., Hou, S., & Zhu, X. M. (2019) · International Journal of Cancer · doi:10.1002/ijc.32762 · Cited by 115
Zhao, S., Xiu, G., Wang, J., Wen, Y., Lu, J., Wu, B., Wang, G., Yang, D., Ling, B., Du, D., et al. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02086-9 · Cited by 109
Exosome-based Ldlr gene therapy for familial hypercholesterolemia in a mouse model
Li, Z., Zhao, P., Zhang, Y., Wang, J., Wang, C., Liu, Y., Yang, G., & Yuan, L. (2021) · Theranostics · doi:10.7150/thno.49874 · Cited by 108
Self-citationEngineered Extracellular Vesicles as a Reliable Tool in Cancer Nanomedicine
Susa, F., Limongi, T., Dumontel, B., Vighetto, V., & Cauda, V. (2019) · Cancers · doi:10.3390/cancers11121979 · Cited by 103
Engineered Extracellular Vesicles: Tailored-Made Nanomaterials for Medical Applications
Man, K., Brunet, M. Y., Jones, M., & Cox, S. C. (2020) · Nanomaterials · doi:10.3390/nano10091838 · Cited by 106
Programming assembly of biomimetic exosomes: An emerging theranostic nanomedicine platform
Xu, X., Xu, L., Wen, C., Xia, J., Zhang, Y., & Liang, Y. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100760 · Cited by 101
Extracellular vesicles versus lipid nanoparticles for the delivery of nucleic acids
Bader, J., Brigger, F., & Leroux, J. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115461 · Cited by 110
Extracellular vesicles in cancer nanomedicine
Tarasov, V. V., Svistunov, A. A., Chubarev, V. N., Dostdar, S. A., Sokolov, A. V., Brzecka, A., Sukocheva, O., Neganova, M. E., Klochkov, S. G., Somasundaram, S. G., et al. (2021) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.08.017 · Cited by 97
Wang, Z., Mo, H., He, Z., Chen, A., & Cheng, P. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.113480 · Cited by 100
The roles of exosomes in cancer drug resistance and its therapeutic application
Li, S., Yi, M., Dong, B., Jiao, Y., Luo, S., & Wu, K. (2020) · Clinical and Translational Medicine · doi:10.1002/ctm2.257 · Cited by 99
CRISPR/Cas9 Delivery System Engineering for Genome Editing in Therapeutic Applications
Cheng, H., Zhang, F., & Ding, Y. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13101649 · Cited by 100
Reprogramming the tumor microenvironment by genome editing for precision cancer therapy
Liu, K., Cui, J., Zhan, Y., Ouyang, Q., Lu, Q., Yang, D., Li, X., & Yin, J. (2022) · Molecular Cancer · doi:10.1186/s12943-022-01561-5 · Cited by 100
Song, H., Chen, X., Hao, Y., Wang, J., Xie, Q., & Wang, X. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01638-9 · Cited by 98
Engineering nanoparticle toolkits for mRNA delivery
Sun, B., Wu, W., Narasipura, E. A., Ma, Y., Yu, C., Fenton, O. S., & Song, H. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115042 · Cited by 96
Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges
Hussen, B. M., Rasul, M. F., Abdullah, S. R., Hidayat, H. J., Faraj, G. S. H., Ali, F. A., Salihi, A. B., Baniahmad, A., Ghafouri‐Fard, S., Rahman, M., et al. (2023) · Military Medical Research · doi:10.1186/s40779-023-00468-6 · Cited by 106
Zhang, H., Yan, W.-J., Wang, J., Xie, S.-Q., Tao, W., Lee, C.-W., Zhang, X., Zhang, G.-Y., Liu, Y.-F., Wei, D., et al. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2024.04.009 · Cited by 88
Organically derived exosomes as carriers of anticancer drugs and imaging agents for cancer treatment
Srivastava, A., Rathore, S., Munshi, A., & Ramesh, R. (2022) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2022.02.020 · Cited by 91
Zhao, X., Fu, L., Zou, H., He, Y., Pan, Y., Ye, L., Huang, Y., Fan, W., Zhang, J., Ma, Y., et al. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-01886-3 · Cited by 87
Strategies for Engineering of Extracellular Vesicles
Aleksandrovna, D. A., Emene, C. C., Barlev, N. A., & Gomzikova, M. O. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241713247 · Cited by 91
Therapeutic Applications of Nanomedicine: Recent Developments and Future Perspectives
Rehan, F., Zhang, M., Fang, J. Q., & Greish, K. F. (2024) · Molecules · doi:10.3390/molecules29092073 · Cited by 97
Extracellular vesicles in cancer´s communication: messages we can read and how to answer
Semerádtová, A., Liegertová, M., Herma, R., Čapková, M., Brignole, C., & Zotto, G. D. (2025) · Molecular Cancer · doi:10.1186/s12943-025-02282-1 · Cited by 95
Exosome‐mediated delivery of miR‐204‐5p inhibits tumor growth and chemoresistance
Yao, S., Yin, Y., Jin, G., Li, D., Li, M., Hu, Y., Feng, Y., Liu, Y., Bian, Z., Wang, X., et al. (2020) · Cancer Medicine · doi:10.1002/cam4.3248 · Cited by 82
CRISPR-Cas9 delivery strategies with engineered extracellular vesicles
Lu, Y., Godbout, K., Lamothe, G., & Tremblay, J. P. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.102040 · Cited by 90
A Novel Peptide-Equipped Exosomes Platform for Delivery of Antisense Oligonucleotides
Xu, H., Liao, C., Liang, S., & Ye, B. (2021) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.1c00016 · Cited by 82
Functional Extracellular Vesicles for Regenerative Medicine
Kim, H. Y., Kwon, S., Um, W., Shin, S., Kim, C. H., Park, J. H., & Kim, B. (2022) · Small · doi:10.1002/smll.202106569 · Cited by 81
Biomass-derived carbon anodes for sodium-ion batteries
Huang, S., Qiu, X., Wang, C., Zhong, L., Zhang, Z.-H., Yang, S.-S., Sun, S., Yang, D.-J., & Zhang, W. (2023) · New Carbon Materials · doi:10.1016/s1872-5805(23)60718-8 · Cited by 80
Song, Z., Tao, Y., Liu, Y., & Li, J. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1444437 · Cited by 81
Extracellular Vesicles – Advanced Nanocarriers in Cancer Therapy: Progress and Achievements
Huyan, T., Li, H., Peng, H., Chen, J., Yang, R., Zhang, W., & Li, Q. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s238099 · Cited by 76
Multiplexed strategies toward clinical translation of extracellular vesicles
Song, J., Song, B., Yuan, L., & Yang, G. (2022) · Theranostics · doi:10.7150/thno.75899 · Cited by 81
Self-citationLiang, Y., Iqbal, Z., Wang, J., Xu, L., Xu, X., Ouyang, K., Zhang, H., Lu, J., Duan, L., & Xia, J. (2022) · Biomaterials Science · doi:10.1039/d2bm00480a · Cited by 72
Exosome based miRNA delivery strategy for disease treatment
Fang, Z., Zhang, X., Huang, H. W., & Wu, J. (2021) · Chinese Chemical Letters · doi:10.1016/j.cclet.2021.11.050 · Cited by 74
Extracellular vesicle‐based nucleic acid delivery
Lu, M., Shao, W., Xing, H., & Huang, Y. (2023) · Interdisciplinary medicine · doi:10.1002/inmd.20220007 · Cited by 75
Nanomaterials for mRNA ‐based therapeutics: Challenges and opportunities
Li, D., Liu, Q., Yang, M., Xu, H., Zhu, M., Zhang, Y., Xu, J., Tian, C., Yao, J., Wang, L., et al. (2023) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10492 · Cited by 74
Converting extracellular vesicles into nanomedicine: loading and unloading of cargo
Joshi, B. S., Ortíz-Rios, D., & Zuhorn, I. S. (2021) · Materials Today Nano · doi:10.1016/j.mtnano.2021.100148 · Cited by 71
Extracellular vesicles for the delivery of gene therapy
Ianni, E. D., Obuchi, W., Breyne, K., & Breakefield, X. O. (2025) · Nature Reviews Bioengineering · doi:10.1038/s44222-025-00277-7 · Cited by 83
Nanotechnology based CRISPR/Cas9 system delivery for genome editing: Progress and prospect
Deng, H., Huang, W., & Zhang, Z. (2019) · Nano Research · doi:10.1007/s12274-019-2465-x · Cited by 70
Engineered extracellular vesicles for bone therapy
Jiang, Y., Li, J., Xu, X. J., Yin, Z., Xu, K., & Su, J. (2022) · Nano Today · doi:10.1016/j.nantod.2022.101487 · Cited by 68
Dave, K. M., Pinky, P. P., & Manickam, D. S. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.114068 · Cited by 80
Exosome-mediated delivery of siRNA molecules in cancer therapy: triumphs and challenges
Ubanako, P., Mirza, S., Ruff, P., & Penny, C. (2024) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2024.1447953 · Cited by 68
Analysis of extracellular vesicles as emerging theranostic nanoplatforms
Xing, Y., Cheng, Z., Wang, R., Lv, C., James, T. D., & Yu, F. (2020) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2020.213506 · Cited by 67
Standardization Approaches for Extracellular Vesicle Loading with Oligonucleotides and Biologics
Roerig, J., & Schulz‐Siegmund, M. (2023) · Small · doi:10.1002/smll.202301763 · Cited by 67
Engineering of MSC-Derived Exosomes: A Promising Cell-Free Therapy for Osteoarthritis
Cheng, J., Sun, Y., Ma, Y., Ao, Y., Hu, X., & Meng, Q. (2022) · Membranes · doi:10.3390/membranes12080739 · Cited by 69
CRISPR-Cas9-Based Technology and Its Relevance to Gene Editing in Parkinson’s Disease
Rahman, M. U., Bilal, M., Shah, J. A., Kaushik, A. K., Teissèdre, P., & Kujawska, M. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14061252 · Cited by 65
Hepatic stellate cell reprogramming via exosome-mediated CRISPR/dCas9-VP64 delivery
Luo, N., Li, J., Chen, Y., Xu, Y., Wei, Y., Lu, J., & Dong, R. (2020) · Drug Delivery · doi:10.1080/10717544.2020.1850917 · Cited by 64
Sun, W., Xing, C., Zhao, L., Zhao, P., Yang, G., & Yuan, L. (2020) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2020.03.016 · Cited by 62
Self-citationGuo, Y., Wan, Z., Zhao, P., Wei, M., Liu, Y., Bu, T., Sun, W., Li, Z., & Yuan, L. (2021) · Journal of Nanobiotechnology · doi:10.1186/s12951-021-01145-3 · Cited by 60
Self-citationThe in vivo fate and targeting engineering of crossover vesicle-based gene delivery system
Jiang, X., Zhang, T., & Gao, J. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114324 · Cited by 61
Metadata
- Authors
- Li, Z., Zhou, X., Wei, M., Gao, X., Zhao, L., Shi, R., Sun, W., Duan, Y., Yang, G., & Yuan, L.
- Affiliations
- Tang Du Hospital (CN)
- Air Force Medical University (CN)
- Corresponding author
- Yang, Guodong
- Yuan, Lijun
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-12-05
- Volume
- 19
- Issue
- 1
- Pages
- 19-28
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2903480993
- Cited by
- 318
- References
- 34
- Citations by year
- 2026: 27 · 2025: 33 · 2024: 54 · 2023: 56 · 2022: 55 · 2021: 43 · 2020: 36 · 2019: 13
- FWCI
- 8.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nano Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81671690, 81871357, 31771507)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- engineered exosomes, RNA delivery, microRNA delivery, CRISPR/dCas9, miR-155, AU-rich elements, RNA-binding proteins, gene therapy vectors
- MeSH terms
- ELAV-Like Protein 1, Animals, Cell Line, Humans, RNA-Binding Proteins, Gene Transfer Techniques, RNA, Small Interfering, MicroRNAs, Mice, Exosomes, Tetraspanin 29, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
