Engineering tumors with 3D scaffolds

Hypoxia — a key regulatory factor in tumour growth

Harris, A. L. (2002) · Nature Reviews Cancer · doi:10.1038/nrc704 · Cited by 5,177

Three-dimensional culture models of normal and malignant breast epithelial cells

Lee, G. Y., Kenny, P. A., Lee, E. H., & Bissell, M. J. (2007) · Nature Methods · doi:10.1038/nmeth1015 · Cited by 1,336

Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink

Pati, F., Jang, J., Ha, D.-H., Won Kim, S., Rhie, J.-W., Shim, J.-H., Kim, D.-H., & Cho, D.-W. (2014) · Nature Communications · doi:10.1038/ncomms4935 · Cited by 1,904

2D and 3D Cell Cultures - a Comparison of Different Types of Cancer Cell Cultures

Kapałczyńska, M., Kolenda, T., Przybyła, W., Zajączkowska, M., Teresiak, A., Filas, V., Ibbs, M., Bliźniak, R., Łuczewski, Ł., & Lamperska, K. (2016) · Archives of Medical Science · doi:10.5114/aoms.2016.63743 · Cited by 1,499

3D Cell Culture Systems: Advantages and Applications

Ravi, M., Paramesh, V., Kaviya, S. R., Anuradha, E., & Solomon, F. D. P. (2015) · Journal of Cellular Physiology · doi:10.1002/jcp.24683 · Cited by 1,206

Organs-on-chips at the frontiers of drug discovery

Esch, E. W., Bahinski, A., & Huh, D. (2015) · Nature Reviews Drug Discovery · doi:10.1038/nrd4539 · Cited by 1,185

Three-Dimensional Cell Culture Matrices: State of the Art

Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047

Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

Vinci, M., Gowan, S., Boxall, F., Patterson, L., Zimmermann, M., Court, W., Lomas, C., Mendiola, M., Hardisson, D., & Eccles, S. A. (2012) · BMC Biology · doi:10.1186/1741-7007-10-29 · Cited by 1,024

3D tumor spheroids: an overview on the tools and techniques used for their analysis

Costa, E. C., Moreira, A. F., de Melo-Diogo, D., Gaspar, V. M., Carvalho, M. P., & Correia, I. J. (2016) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.11.002 · Cited by 929

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2014.0086 · Cited by 611

Axl is an essential epithelial-to-mesenchymal transition-induced regulator of breast cancer metastasis and patient survival

Gjerdrum, C., Tiron, C., Høiby, T., Stefansson, I., Haugen, H., Sandal, T., Collett, K., Li, S., McCormack, E., Gjertsen, B. T., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909333107 · Cited by 594

Advances in multicellular spheroids formation

Cui, X., Hartanto, Y., & Zhang, H. (2017) · Journal of The Royal Society Interface · doi:10.1098/rsif.2016.0877 · Cited by 508

Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research

Pinto, B., Henriques, A. C., Silva, P. M. A., & Bousbaa, H. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12121186 · Cited by 440

The production of 3D tumor spheroids for cancer drug discovery

Sant, S., & Johnston, P. A. (2017) · Drug Discovery Today: Technologies · doi:10.1016/j.ddtec.2017.03.002 · Cited by 404

Biomaterials and emerging anticancer therapeutics: engineering the microenvironment

Gu, L., & Mooney, D. J. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2015.3 · Cited by 407

Structurally Dynamic Hydrogels for Biomedical Applications: Pursuing a Fine Balance between Macroscopic Stability and Microscopic Dynamics

Zhang, K., Feng, Q., Fang, Z., Gu, L., & Bian, L. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00071 · Cited by 394

Engineering microscale cellular niches for three-dimensional multicellular co-cultures

Huang, C. P., Lu, J., Seon, H., Lee, A. P., Flanagan, L. A., Kim, H.-Y., Putnam, A. J., & Jeon, N. L. (2009) · Lab on a Chip · doi:10.1039/b818401a · Cited by 384

Organ-on-a-chip platforms for studying drug delivery systems

Bhise, N. S., Ribas, J., Manoharan, V., Zhang, Y. S., Polini, A., Massa, S., Dokmeci, M. R., & Khademhosseini, A. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.05.004 · Cited by 381

Generation of Multicellular Tumor Spheroids with Microwell-Based Agarose Scaffolds for Drug Testing

Gong, X., Lin, C., Cheng, J., Su, J., Zhao, H., Liu, T., Wen, X., & Zhao, P. (2015) · PLOS ONE · doi:10.1371/journal.pone.0130348 · Cited by 373

Biomaterials offer cancer research the third dimension

Hutmacher, D. W. (2010) · Nature Materials · doi:10.1038/nmat2619 · Cited by 323

Engineering cancer microenvironments for in vitro 3-D tumor models

Asghar, W., El Assal, R., Shafiee, H., Pitteri, S., Paulmurugan, R., & Demirci, U. (2015) · Materials Today · doi:10.1016/j.mattod.2015.05.002 · Cited by 316

Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells

Gurski, L. A., Jha, A. K., Zhang, C., Jia, X., & Farach-Carson, M. C. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.07.054 · Cited by 269

3D Bioprinted in vitro metastatic models via reconstruction of tumor microenvironments

Meng, F., Meyer, C. M., Joung, D., Vallera, D. A., McAlpine, M. C., & Panoskaltsis‐Mortari, A. (2019) · Advanced Materials · doi:10.1002/adma.201806899 · Cited by 262

Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

Tsai, H.-F., Trubelja, A., Shen, A. Q., & Bao, G. (2017) · Journal of The Royal Society Interface · doi:10.1098/rsif.2017.0137 · Cited by 214

Chitosan–alginate 3D scaffolds as a mimic of the glioma tumor microenvironment

Kievit, F. M., Florczyk, S. J., Leung, M. C., Veiseh, O., Park, J. O., Disis, M. L., & Zhang, M. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.062 · Cited by 209

3D tumor models: History, advances and future perspectives

Benien, P., & Swami, A. (2014) · Future Oncology · doi:10.2217/fon.13.274 · Cited by 208

Generation and functional assessment of 3D multicellular spheroids in droplet based microfluidics platform

Sabhachandani, P., Motwani, V., Cohen, N., Sarkar, S., Torchilin, V., & Konry, T. (2016) · Lab on a Chip · doi:10.1039/c5lc01139f · Cited by 193

Embedded multicellular spheroids as a biomimetic 3D cancer model for evaluating drug and drug-device combinations

Charoen, K. M., Fallica, B., Colson, Y. L., Zaman, M. H., & Grinstaff, M. W. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.038 · Cited by 187

3D scaffolds in breast cancer research

Rijal, G., & Li, W. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2015.12.016 · Cited by 169

Simulation of complex transport of nanoparticles around a tumor using tumor-microenvironment-on-chip

Kwak, B., Ozcelikkale, A., Shin, C. S., Park, K., & Han, B. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.08.027 · Cited by 165

Modeling human carcinomas: Physiologically relevant 3D models to improve anti-cancer drug development

Unger, C., Kramer, N., Walzl, A., Scherzer, M., Hengstschläger, M., & Dolznig, H. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.10.015 · Cited by 158

A versatile 3D tissue matrix scaffold system for tumor modeling and drug screening

Rijal, G., & Li, W. (2017) · Science Advances · doi:10.1126/sciadv.1700764 · Cited by 154

Hydrogels to model 3D in vitro microenvironment of tumor vascularization

Song, H.-H. G., Park, K. M., & Gerecht, S. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.06.002 · Cited by 153

The resistance of intracellular mediators to doxorubicin and cisplatin are distinct in 3D and 2D endometrial cancer

Chitcholtan, K., Sykes, P. H., & Evans, J. J. (2012) · Journal of Translational Medicine · doi:10.1186/1479-5876-10-38 · Cited by 148

Three-dimensional microfluidic collagen hydrogels for investigating flow-mediated tumor-endothelial signaling and vascular organization

Buchanan, C. F., Voigt, E. E., Szot, C. S., Freeman, J. W., Vlachos, P. P., & Rylander, M. N. (2014) · Tissue Engineering Part C: Methods · doi:10.1089/ten.tec.2012.0731 · Cited by 143

New dimensions in tumor immunology: what does 3D culture reveal?

Feder-Mengus, C., Ghosh, S., Reschner, A., Martin, I., & Spagnoli, G. C. (2008) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2008.06.001 · Cited by 139

Development of an in Vitro 3D Tumor Model to Study Therapeutic Efficiency of an Anticancer Drug

Shin, C. S., Kwak, B., Han, B., & Park, K. (2013) · Molecular Pharmaceutics · doi:10.1021/mp300595a · Cited by 135

A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients

Rodenhizer, D., Gaude, E., Cojocari, D., Mahadevan, R., Frezza, C., Wouters, B. G., & McGuigan, A. P. (2016) · Nature Materials · doi:10.1038/nmat4482 · Cited by 132

Extracellular Matrix Remodeling and Stiffening Modulate Tumor Phenotype and Treatment Response

Leight, J. L., Drain, A. P., & Weaver, V. M. (2017) · Annual Review of Cancer Biology · doi:10.1146/annurev-cancerbio-050216-034431 · Cited by 131

3D Matrices for Anti-Cancer Drug Testing and Development

Gurski, L. A., Petrelli, N. J., Jia, X., & Farach-Carson, M. C. (2010) · Oncology Issues · doi:10.1080/10463356.2010.11883480 · Cited by 130

A heterogeneous in vitro three dimensional model of tumour-stroma interactions regulating sprouting angiogenesis

Correa de Sampaio, P., Auslaender, D., Krubasik, D., Failla, A. V., Skepper, J. N., Murphy, G., & English, W. R. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030753 · Cited by 127

Incorporation of multicellular spheroids into 3‐D polymeric scaffolds provides an improved tumor model for screening anticancer drugs

Ho, W. J., Pham, E. A., Kim, J. W., Ng, C. W., Kim, J. H., Kamei, D. T., & Wu, B. M. (2010) · Cancer Science · doi:10.1111/j.1349-7006.2010.01723.x · Cited by 115

Three-Dimensional Microfluidic Tri-Culture Model of the Bone Marrow Microenvironment for Study of Acute Lymphoblastic Leukemia

Bruce, A., Evans, R., Mezan, R., Shi, L., Moses, B. S., Martin, K. H., Gibson, L. F., & Yang, Y. (2015) · PLOS ONE · doi:10.1371/journal.pone.0140506 · Cited by 112

Characterizing the role of monocytes in T cell cancer immunotherapy using a 3d microfluidic model

Lee, S. W. L., Adriani, G., Ceccarello, E., Pavesi, A., Tan, A. T., Bertoletti, A., Kamm, R. D., & Wong, S. C. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.00416 · Cited by 109

Engineering bioprintable alginate/gelatin composite hydrogels with tunable mechanical and cell adhesive properties to modulate tumor spheroid growth kinetics

Jiang, T., Munguia-Lopez, J. G., Gu, K., Bavoux, M. M., Flores-Torres, S., Kort-Mascort, J., Grant, J., Vijayakumar, S., De Leon-Rodriguez, A., Ehrlicher, A. J., et al. (2020) · Biofabrication · doi:10.1088/1758-5090/ab3a5c · Cited by 106

Tissue-engineered 3D tumor angiogenesis models: Potential technologies for anti-cancer drug discovery

Chwalek, K., Bray, L. J., & Werner, C. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.05.006 · Cited by 91

Modeling the tumor extracellular matrix: tissue engineering tools repurposed towards new frontiers in cancer biology

Gill, B. J., & West, J. L. (2014) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2013.09.029 · Cited by 86

Cyfip1 is a putative invasion suppressor in epithelial cancers

Silva, J. M., Ezhkova, E., Silva, J., Heart, S., Castillo, M., Campos, Y., Castro, V., Bonilla, F., Cordon-Cardo, C., Muthuswamy, S. K., et al. (2009) · Cell · doi:10.1016/j.cell.2009.04.013 · Cited by 87

The current landscape of 3D in vitro tumor models: what cancer hallmarks are accessible for drug discovery?

Rodenhizer, D., Dean, T., D'Arcangelo, E., & McGuigan, A. P. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701174 · Cited by 80

Peptide-protein Coassembling Matrices as a Biomimetic 3D Model of Ovarian Cancer

Hedegaard, C. L., Redondo-Gómez, C., Tan, B. Y., Ng, K. W., Loessner, D., & Mata, A. (2020) · Science Advances · doi:10.1126/sciadv.abb3298 · Cited by 76

In vitro vascularized liver and tumor tissue microenvironments on a chip for dynamic determination of nanoparticle transport and toxicity

Ozkan, A., Ghousifam, N., Hoopes, P. J., Yankeelov, T. E., & Rylander, M. N. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.26919 · Cited by 73

In vitro modeling of the prostate cancer microenvironment

Ellem, S. J., De-Juan-Pardo, E. M., & Risbridger, G. P. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.04.008 · Cited by 68

Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds

Lv, Y., Wang, H., Li, G., & Zhao, B. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.02.004 · Cited by 67

Recapitulation of complex transport and action of drugs at the tumor microenvironment using tumor-microenvironment-on-chip

Han, B., Qu, C., Park, K., Konieczny, S. F., & Korc, M. (2016) · Cancer Letters · doi:10.1016/j.canlet.2015.12.003 · Cited by 53

The Anti-Angiogenic Effect of Atorvastatin in Glioblastoma Spheroids Tumor Cultured in Fibrin Gel: in 3D in Vitro Model

(2018) · Europe PMC (PubMed Central) · doi:10.22034/apjcp.2018.19.9.2553 · Cited by 44

Biomarkers for simplifying HTS 3D cell culture platforms for drug discovery: the case for cytokines

Lai, Y., Asthana, A., & Kisaalita, W. S. (2011) · Drug Discovery Today · doi:10.1016/j.drudis.2011.01.009 · Cited by 43

Microfluidic system for modelling 3D tumour invasion into surrounding stroma and drug screening

Du, Z., Mi, S., Yi, X., Xu, Y., & Sun, W. (2018) · Biofabrication · doi:10.1088/1758-5090/aac70c · Cited by 42

Microfluidic Biopsy Trapping Device for the Real-Time Monitoring of Tumor Microenvironment

Holton, A. B., Sinatra, F. L., Kreahling, J., Conway, A. J., Landis, D. A., & Altiok, S. (2017) · PLOS ONE · doi:10.1371/journal.pone.0169797 · Cited by 35

3D Tumor Spheroid Models for In Vitro Therapeutic Screening of Nanoparticles

Daunys, S., Janonienė, A., Januškevičienė, I., Paškevičiūtė, M., & Petrikaitė, V. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-58174-9_11 · Cited by 32

Deriving Primary Cancer Cell Cultures for Personalized Therapy

Esparza-López, J., Martínez-Aguilar, J. F., & Ibarra-Sánchez, M. D. J. (2019) · Revista de Investigación Clínica · doi:10.24875/ric.19002832 · Cited by 20