Suppressed proliferation and apoptotic changes in the rat dentate gyrus after acute and chronic stress are reversible
Abstract
Acute stress suppresses new cell birth in the hippocampus in several species. Relatively little is known, however, on how chronic stress affects the turnover, i.e. proliferation and apoptosis, of the rat dentate gyrus (DG) cells, and whether the stress effects are lasting. We investigated how 3 weeks of chronic unpredictable stress would influence the structural dynamic plasticity of the rat DG, and studied newborn cell proliferation, survival, apoptosis, volume and cell number in 10‐week‐old animals. To study lasting effects, another group of animals was allowed to recover for 3 weeks. Based on two independent parameters, bromodeoxyuridine (BrdU) and Ki‐67 immunocytochemistry, our results show that both chronic and acute stress decrease new cell proliferation rate. The reduced proliferation after acute stress normalized within 24 h. Interestingly, chronically stressed animals showed recovery after 3 weeks, albeit with still fewer proliferating cells than controls. Apoptosis, by contrast, increased after acute but decreased after chronic stress. These results demonstrate that, although chronic stress suppresses proliferation and apoptosis, 3 weeks of recovery again normalized most of these alterations. This may have important implications for our understanding of the reversibility of stress‐related hippocampal volume changes, such as occur, for example, in depression.
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There was no evidence from TUNEL staining that IL-1β and/or acute stress elicit hippocampal cell death either in vitro or in vivo.
“In the present study, there was no evidence from TUNEL staining that IL-1β and/or acute stress elicit hippocampal cell death either in vitro or in vivo, which is consistent with previous studies (40–42), although there is one report that severe acute stress increases apoptosis (43).”
From IL-1β is an essential mediator of the antineurogenic and anhedonic effects of stress (2008) · cites this paper as [43]
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- Authors
- Heine, V. M., Maslam, S., Zareno, J., Joëls, M., & Lucassen, P. J.
- Affiliations
- University of Amsterdam (NL)
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 2003-12-16
- Volume
- 19
- Issue
- 1
- Pages
- 131-144
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2063394935
- Cited by
- 317
- References
- 15
- Citations by year
- 2025: 2 · 2024: 5 · 2023: 7 · 2022: 6 · 2021: 7 · 2020: 7 · 2019: 14 · 2018: 13 · 2017: 14 · 2016: 17 · 2015: 13 · 2014: 20 · 2013: 23 · 2012: 15
- FWCI
- 3.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- chronic stress, acute stress, dentate gyrus, hippocampus, neurogenesis, cell proliferation, apoptosis, stress recovery, structural plasticity, hippocampal volume, depression
- MeSH terms
- Acute Disease, Animals, Bromodeoxyuridine, Cell Division, Chronic Disease, Male, Nerve Degeneration, Neuronal Plasticity, Stress, Physiological, Rats, Wistar, Apoptosis, Dentate Gyrus, Ki-67 Antigen, Recovery of Function, Rats
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