Cited by 326Regulation of Appetite and ObesityHypothalamic control of reproductive hormonesNeuroscience and Neuropharmacology Research

Rapid sensing of circulating ghrelin by hypothalamic appetite-modifying neurons

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Schaeffer, Marie, Langlet, Fanny, Lafont, Chrystel, Molino, François, Hodson, David J., Roux, Thomas, Lamarque, Laurent, Verdié, Pascal +10 more·2013·Proceedings of the National Academy of Sciences

Abstract

To maintain homeostasis, hypothalamic neurons in the arcuate nucleus must dynamically sense and integrate a multitude of peripheral signals. Blood-borne molecules must therefore be able to circumvent the tightly sealed vasculature of the blood-brain barrier to rapidly access their target neurons. However, how information encoded by circulating appetite-modifying hormones is conveyed to central hypothalamic neurons remains largely unexplored. Using in vivo multiphoton microscopy together with fluorescently labeled ligands, we demonstrate that circulating ghrelin, a versatile regulator of energy expenditure and feeding behavior, rapidly binds neurons in the vicinity of fenestrated capillaries, and that the number of labeled cell bodies varies with feeding status. Thus, by virtue of its vascular connections, the hypothalamus is able to directly sense peripheral signals, modifying energy status accordingly.

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  • Peripherally produced ghrelin exerts appetite-inducing effects centrally after passing through the BBB.

    “Peripherally produced ghrelin exerts its appetite-inducing effects centrally after passing through the BBB [112, 302-304].”

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Authors
Schaeffer, M., Langlet, F., Lafont, C., Molino, F., Hodson, D. J., Roux, T., Lamarque, L., Verdié, P., Bourrier, E., Dehouck, B., Banères, J.-L., Martinez, J., Méry, P.-F., Marie, J., Trinquet, E., Fehrentz, J.-A., Prévot, V., & Mollard, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Inserm (FR)
  • Cisbio (France) (FR)
  • Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5247, Institut des Biomolécules Max Mousseron, Faculty of Pharmacy, Universities of Montpellier 1 and 2, F-34093 Montpellier Cedex 5, France; and
Venue
Proceedings of the National Academy of Sciences
Published
2013-01-07
Volume
110
Issue
4
Pages
1512-1517
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2152571442
Cited by
325
References
46
Citations by year
2026: 13 · 2025: 22 · 2024: 25 · 2023: 17 · 2022: 28 · 2021: 25 · 2020: 28 · 2019: 19 · 2018: 26 · 2017: 28 · 2016: 25 · 2015: 28 · 2014: 26 · 2013: 14
FWCI
16.69
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
ghrelin, hypothalamus, arcuate nucleus, blood-brain barrier, fenestrated capillaries, feeding behavior, energy homeostasis, neuropeptide binding
MeSH terms
Animals, Appetite Regulation, Blood-Brain Barrier, Capillary Permeability, Eating, Fasting, Hypothalamus, Male, Median Eminence, Mice, Inbred C57BL, Mice, Transgenic, Models, Neurological, Neurons, Microscopy, Fluorescence, Multiphoton, Mice, Ghrelin

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