microRNAs expression in endometriosis and their relation to angiogenic factors
Abstract
BACKGROUND: Endometriosis is a common, multifactorial disease in which angiogenesis may be involved in the growth of endometrium outside the uterus. microRNAs (miRNAs) are 21-22 nucleotide non-coding RNAs that regulate gene expression and play fundamental roles in biological processes. The objective of this study was to analyze several miRNAs related to angiogenesis and the angiogenic factors, vascular endothelial growth factor-A (VEGF-A) and thrombospondin-1 (TSP-1), in endometriotic lesions (ovarian endometrioma, peritoneal lesion and rectovaginal nodule) and eutopic endometrium from women with endometriosis. METHODS: TaqMan real-time PCR was used to assess the expression of the miRNAs (miR-15b, -16, -17-5p, -20a, -21, -125a, -221 and -222), while VEGF-A and TSP-1 mRNA were assessed by real-time PCR, with SYBR Green I and VEGF-A and TSP-1 protein levels were quantified by ELISA. Included in the study were 58 women with endometriosis and 38 control women. RESULTS: In paired samples, ovarian endometrioma showed significantly lower VEGF-A mRNA (P = 0.02) and protein (P = 0.002) expression than eutopic endometrium and higher expression of miR-125a (P = 0.003) and miR-222 (P <0.001). However, ovarian endometrioma had significantly higher expression of the angiogenic inhibitor TSP-1 and lower expression of miR-17-5p than eutopic endometrium (P < 0.001). Moreover, a significant inverse correlations between miR-222 and VEGF-A protein levels (-0.267, P = 0.018) and between miR-17-5p and TSP-1 protein levels (-0.260, P=0.022) were observed. Peritoneal lesions showed a significant increase in VEGF-A in comparison with ovarian endometrioma (P < 0.01). CONCLUSIONS: Expression levels of miRNAs related to angiogenesis were different in eutopic endometrium from that observed in ovarian endometrioma. This could influence the expression of angiogenic factors and play a role in the pathogenesis of endometriosis.
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Expression levels of miRNAs related to angiogenesis were different in eutopic endometrium from that observed in ovarian endometrioma.
“Other workers have shown that expression levels of miRNAs related to angiogenesis were different in eutopic endometrium from that observed in ovarian endometrioma.”
From Defining Future Directions for Endometriosis Research: Workshop Report From the 2011 World Congress of Endometriosis in Montpellier, France (2013) · cites this paper as [114]
These differences could influence the expression of angiogenic factors and play a role in the pathogenesis of endometriosis.
“They conclude that this could influence the expression of angiogenic factors and play a role in the pathogenesis of endometriosis.114”
From Defining Future Directions for Endometriosis Research: Workshop Report From the 2011 World Congress of Endometriosis in Montpellier, France (2013) · cites this paper as [114]
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- Authors
- Ramon, L. A., Braza-Boils, A., Gilabert-Estelles, J., Gilabert, J., Espana, F., Chirivella, M., & Estelles, A.
- Affiliations
- Hospital Arnau de Vilanova (ES)
- Hospital Universitari i Politècnic La Fe (ES)
- Venue
- Human Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-02-18
- Volume
- 26
- Issue
- 5
- Pages
- 1082-1090
- ISSN
- 0268-1161 (ISSN-L)
- 1460-2350
- OpenAlex
- W2127311828
- Cited by
- 117
- References
- 46
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 10 · 2021: 7 · 2020: 11 · 2019: 9 · 2018: 6 · 2017: 8 · 2016: 8 · 2015: 12 · 2014: 5 · 2013: 13 · 2012: 8
- FWCI
- 10.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- endometriosis, microRNA, angiogenesis, VEGF-A, thrombospondin-1, endometrioma, peritoneal endometriosis, eutopic endometrium, miR-17-5p, miR-222, miR-125a, miR-15b, miR-16, miR-21, miR-20a
- MeSH terms
- Adult, Endometriosis, Enzyme-Linked Immunosorbent Assay, Female, Gene Expression Regulation, Humans, Menstrual Cycle, Neovascularization, Pathologic, RNA, Messenger, Thrombospondin 1, MicroRNAs, Vascular Endothelial Growth Factor A, Angiogenesis Inducing Agents
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