The DOI matches its OpenAlex record; 3 of the 3 references it cites were found in a registry or an index.
Expression of IGF-I splice variants in young and old human skeletal muscle after high resistance exercise
Open accessAbstract
The mRNA expression of two splice variants of the insulin-like growth factor-I (IGF-I) gene, IGF-IEa and mechano growth factor (MGF), were studied in human skeletal muscle. Subjects (eight young, aged 25-36 years, and seven elderly, aged 70-82 years) completed 10 sets of six repetitions of single legged knee extensor exercise at 80 % of their one repetition maximum. Muscle biopsy samples were obtained from the quadriceps muscle of both the control and exercised legs 2.5 h after completion of the exercise bout. Expression levels of the IGF-I mRNA transcripts were determined using real-time quantitative RT-PCR with specific primers. The resting levels of MGF were significantly (approximately 100-fold) lower than those of the IGF-IEa isoform. No difference was observed between the resting levels of the two isoforms between the two subject groups. High resistance exercise resulted in a significant increase in MGF mRNA in the young, but not in the elderly subjects. No changes in IGF-IEa mRNA levels were observed as a result of exercise in either group. The mRNA levels of the transcription factor MyoD were greater at rest in the older subjects (P < 0.05), but there was no significant effect of the exercise bout. Electrophoretic separation of myosin heavy chain (MHC) isoforms showed the older subjects to have a lower (P < 0.05) percentage of MHC-II isoforms than the young subjects. However, no association was observed between the composition of the muscle and changes in the IGF-I isoforms with exercise. The data from this study show an attenuated MGF response to high resistance exercise in the older subjects, indicative of age-related desensitivity to mechanical loading. The data in young subjects indicate that the MGF and IGF-IEa isoforms are differentially regulated in human skeletal muscle.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Exercise training and nutritional supplementation for physical frailty in very elderly people
Fiatarone, M. A., O'Neill, E. F., Ryan, N. D., Clements, K. M., Solares, G. R., Nelson, M. E., Roberts, S. B., Kehayias, J. J., Lipsitz, L. A., & Evans, W. J. (1994) · New England Journal of Medicine · doi:10.1056/nejm199406233302501 · Cited by 2,857
Musarò, A., McCullagh, K., Paul, A., Houghton, L., Dobrowolny, G., Molinaro, M., Barton, E. R., L Sweeney, H., & Rosenthal, N. (2001) · Nature Genetics · doi:10.1038/84839 · Cited by 1,137 · cited 2×
“It is the cDNA of this isoform that has been used for gene transfer into muscles or to generate transgenic mice (Barton-Davies et al. 1998; Musaro et al. 2001).”
BackgroundAagaard, P., Andersen, J. L., Dyhre‐Poulsen, P., Leffers, A., Wagner, A., Magnusson, S. P., Halkjær‐Kristensen, J., & Simonsen, E. B. (2001) · The Journal of Physiology · doi:10.1111/j.1469-7793.2001.t01-1-00613.x · Cited by 733 · cited 1×
“The hypertrophic response to strength training exercise has been reported to be greater in type II fibres which express the MHC-IIa and IIX isoforms (Tesch et al. 1985; Kadi et al. 1999; Aagaard et al. 2001; Andersen & Aagaard, 2000).”
Background
Cited by
Exercise and Physical Activity for Older Adults
Chodzko-Zajko, W. J., Proctor, D. N., Fiatarone Singh, M. A., Minson, C. T., Nigg, C. R., Salem, G. J., & Skinner, J. S. (2009) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e3181a0c95c · Cited by 5,153
Hormonal Responses and Adaptations to Resistance Exercise and Training
Kraemer, W. J., & Ratamess, N. A. (2005) · Sports Medicine · doi:10.2165/00007256-200535040-00004 · Cited by 1,389
Fragala, M. S., Cadore, E. L., Dorgo, S., Izquierdo, M., Kraemer, W. J., Peterson, M. D., & Ryan, E. D. (2019) · Journal of Strength and Conditioning Research · doi:10.1519/jsc.0000000000003230 · Cited by 1,290
Effects of Resistance Training on Older Adults
Hunter, G. R., McCarthy, J. P., & Bamman, M. M. (2004) · Sports Medicine · doi:10.2165/00007256-200434050-00005 · Cited by 764
The Molecular Bases of Training Adaptation
Coffey, V. G., & Hawley, J. A. (2007) · Sports Medicine · doi:10.2165/00007256-200737090-00001 · Cited by 756
Effects of Aging on Muscle Fibre Type and Size
Deschenes, M. R. (2004) · Sports Medicine · doi:10.2165/00007256-200434120-00002 · Cited by 671
Oxidative Stress, Molecular Inflammation and Sarcopenia
Meng, S.-J., & Yu, L.-J. (2010) · International Journal of Molecular Sciences · doi:10.3390/ijms11041509 · Cited by 637
“It is resistance exercise that promotes muscle hypertrophy in young and middle-aged individuals [90].”
BackgroundWernbom, M., Augustsson, J., & Thome??, R. (2007) · Sports Medicine · doi:10.2165/00007256-200737030-00004 · Cited by 583
New fundamental resistance exercise determinants of molecular and cellular muscle adaptations
Toigo, M., & Boutellier, U. (2006) · European Journal of Applied Physiology · doi:10.1007/s00421-006-0238-1 · Cited by 437
Cellular and molecular mechanisms underlying age-related skeletal muscle wasting and weakness
Ryall, J. G., Schertzer, J. D., & Lynch, G. S. (2008) · Biogerontology · doi:10.1007/s10522-008-9131-0 · Cited by 423
Control of the size of the human muscle mass
Rennie, M. J., Wackerhage, H., Spangenburg, E. E., & Booth, F. W. (2004) · Annual Review of Physiology · doi:10.1146/annurev.physiol.66.052102.134444 · Cited by 399
Sarcopenia, frailty and their prevention by exercise
Nascimento, C. M., Ingles, M., Salvador-Pascual, A., Cominetti, M. R., Gomez-Cabrera, M. C., & Viña, J. (2019) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2018.08.035 · Cited by 396
Skeletal Muscle as an Endocrine Organ: The Role of Myokines in Exercise Adaptations
Hoffmann, C., & Weigert, C. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a029793 · Cited by 393
Aging, exercise, and muscle protein metabolism
Koopman, R., & van Loon, L. J. C. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91551.2008 · Cited by 371
Stimuli and sensors that initiate skeletal muscle hypertrophy following resistance exercise
Wackerhage, H., Schoenfeld, B. J., Hamilton, D. L., Lehti, M., & Hulmi, J. J. (2019) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00685.2018 · Cited by 357
Possible Stimuli for Strength and Power Adaptation: Acute Mechanical Responses
Crewther, B., Cronin, J., & Keogh, J. (2005) · Sports Medicine · doi:10.2165/00007256-200535110-00004 · Cited by 336
Effects of Resistance Exercise on Bone Health
Hong, A. R., & Kim, S. W. (2018) · Endocrinology and Metabolism · doi:10.3803/enm.2018.33.4.435 · Cited by 291
Yang, Y., Creer, A., Jemiolo, B., & Trappe, S. (2005) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01185.2004 · Cited by 290
Egan, B., & Sharples, A. P. (2023) · Physiological Reviews · doi:10.1152/physrev.00054.2021 · Cited by 278
Satellite cell numbers in young and older men 24 hours after eccentric exercise
Dreyer, H. C., Blanco, C. E., Sattler, F. R., Schroeder, E. T., & Wiswell, R. A. (2006) · Muscle & Nerve · doi:10.1002/mus.20461 · Cited by 256
Lambert, C. P., Wright, N. R., Finck, B. N., & Villareal, D. T. (2008) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00006.2008 · Cited by 249
Roberts, M. D., McCarthy, J. J., Hornberger, T. A., Phillips, S. M., Mackey, A. L., Nader, G. A., Boppart, M. D., Kavazis, A. N., Reidy, P. T., Ogasawara, R., et al. (2023) · Physiological Reviews · doi:10.1152/physrev.00039.2022 · Cited by 214
Bamman, M. M., Petrella, J. K., Kim, J.-S., Mayhew, D. L., & Cross, J. M. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00024.2007 · Cited by 216
Dietary protein requirements and adaptive advantages in athletes
Phillips, S. M. (2012) · British Journal Of Nutrition · doi:10.1017/s0007114512002516 · Cited by 199
Raue, U., Slivka, D., Minchev, K., & Trappe, S. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91587.2008 · Cited by 166
Modifying muscle mass – the endocrine perspective
Solomon, A. M., & Bouloux, P. M. G. (2006) · Journal of Endocrinology · doi:10.1677/joe.1.06837 · Cited by 156
Hormone Replacement Therapy and Physical Function in Healthy Older Men. Time to Talk Hormones?
Giannoulis, M. G., Martin, F. C., Nair, K. S., Umpleby, A. M., & Sonksen, P. (2012) · Endocrine Reviews · doi:10.1210/er.2012-1002 · Cited by 132
Coffey, V. G., Jemiolo, B., Edge, J., Garnham, A. P., Trappe, S. W., & Hawley, J. A. (2009) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00351.2009 · Cited by 120
Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle
Coffey, V. G., Pilegaard, H., Garnham, A. P., O'Brien, B. J., & Hawley, J. A. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.91221.2008 · Cited by 116
Powerful signals for weak muscles
Saini, A., Faulkner, S., Al-Shanti, N., & Stewart, C. (2009) · Ageing Research Reviews · doi:10.1016/j.arr.2009.02.001 · Cited by 109
Roberts, M. D., Haun, C. T., Mobley, C. B., Mumford, P. W., Romero, M. A., Roberson, P. A., Vann, C. G., & McCarthy, J. J. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.00834 · Cited by 107
Churchley, E. G., Coffey, V. G., Pedersen, D. J., Shield, A., Carey, K. A., Cameron-Smith, D., & Hawley, J. A. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01260.2006 · Cited by 80
Pugh, J. K., Faulkner, S. H., Jackson, A. P., King, J. A., & Nimmo, M. A. (2015) · Physiological Reports · doi:10.14814/phy2.12364 · Cited by 63
Muscle health and performance in monozygotic twins with 30 years of discordant exercise habits.
Bathgate, K. E., Bagley, J. R., Jo, E., Talmadge, R. J., Tobias, I. S., Brown, L. E., Coburn, J. W., Arevalo, J. A., Segal, N. L., & Galpin, A. J. (2018) · European Journal of Applied Physiology · doi:10.1007/s00421-018-3943-7 · Cited by 53
Hand, B. D., Kostek, M. C., Ferrell, R. E., Delmonico, M. J., Douglass, L. W., Roth, S. M., Hagberg, J. M., & Hurley, B. F. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00420.2007 · Cited by 53
Dietary protein and exercise training in ageing
Koopman, R. (2011) · Proceedings of The Nutrition Society · doi:10.1017/s0029665110003927 · Cited by 50
Impact of physical exercise on endocrine aging
Janssen, J. A. M. J. L. (2016) · Frontiers of hormone research · doi:10.1159/000445158 · Cited by 37
Babcock, L., Escano, M., D'Lugos, A., Todd, K., Murach, K., & Luden, N. (2012) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00035.2012 · Cited by 34
Nutrition for Older Athletes: Focus on Sex-Differences
Strasser, B., Pesta, D., Rittweger, J., Burtscher, J., & Burtscher, M. (2021) · Nutrients · doi:10.3390/nu13051409 · Cited by 25
Aging alters the reduction of pro-apoptotic signaling in response to loading-induced hypertrophy
Siu, P. M., & Alway, S. E. (2006) · Experimental Gerontology · doi:10.1016/j.exger.2005.11.004 · Cited by 24
Metadata
- Authors
- Hameed, M., Orrell, R. W., COBBOLD, M., Goldspink, G., & Harridge, S. D. R.
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2002-12-20
- Volume
- 547
- Issue
- 1
- Pages
- 247-254
- ISSN
- 0022-3751 (ISSN-L)
- OpenAlex
- W2156148379
- Cited by
- 416
- References
- 3
- FWCI
- 7.02
- Type
- journal article
- Language
- en
- Open access
- green
- Licence
- doi.wiley.com/10.1002/tdm_license_1 (tdm, 4638 d)
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
