Cited by 258DNA Repair MechanismsFungal and yeast genetics researchBacterial Genetics and Biotechnology

Recruitment of Mec1 and Ddc1 Checkpoint Proteins to Double-Strand Breaks Through Distinct Mechanisms

Kondo, Tae, Wakayama, Tatsushi, Naiki, Takahiro, Matsumoto, Kunihiro, Sugimoto, Katsunori·2001·Science

Abstract

In response to DNA damage, eukaryotic cells activate checkpoint pathways that arrest cell cycle progression and induce the expression of genes required for DNA repair. In budding yeast, the homothallic switching (HO) endonuclease creates a site-specific double-strand break at the mating type ( MAT ) locus. Continuous HO expression results in the phosphorylation of Rad53, which is dependent on products of the ataxia telangiectasia mutated–related MEC1 gene and other checkpoint genes, including DDC1 , RAD9 , and RAD24 . Chromatin immunoprecipitation experiments revealed that the Ddc1 protein associates with a region near the MAT locus after HO expression. Ddc1 association required Rad24 but not Mec1 or Rad9. Mec1 also associated with a region near the cleavage site after HO expression, but this association is independent of Ddc1, Rad9, and Rad24. Thus, Mec1 and Ddc1 are recruited independently to sites of DNA damage, suggesting the existence of two separate mechanisms involved in recognition of DNA damage.

Integrity signals

Registry

Crossref · 10.1126/science.1063827

Cites

25 of 25 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Kondo, T., Wakayama, T., Naiki, T., Matsumoto, K., & Sugimoto, K.
Affiliations
  • Nagoya University (JP)
  • Japan Science and Technology Agency (JP)
Corresponding author
  • Kondo, Tae
  • Wakayama, Tatsushi
  • Naiki, Takahiro
  • Sugimoto, Katsunori
Venue
Science
Published
2001-10-26
Volume
294
Issue
5543
Pages
867-870
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2014438192
Cited by
258
References
25
Citations by year
2026: 3 · 2025: 1 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 3 · 2016: 6 · 2015: 7 · 2014: 8 · 2013: 3 · 2012: 4
FWCI
12.92
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Mec1, DNA damage checkpoint, DNA double-strand breaks, Rad53 phosphorylation, HO endonuclease, mating-type locus, Rad24, Rad9, DNA damage recognition, Saccharomyces cerevisiae
MeSH terms
Mating Factor, Cell Cycle, Cell Nucleus, Cytoplasm, DNA Damage, DNA Repair, DNA-Binding Proteins, DNA, Fungal, Saccharomycetales, Fungal Proteins, Genes, Fungal, Mutation, Nuclear Proteins, Peptides, Phosphorylation, Recombinant Fusion Proteins, Recombination, Genetic, Transformation, Genetic, Deoxyribonucleases, Type II Site-Specific, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Genes, cdc, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins, Genes, Mating Type, Fungal, Checkpoint Kinase 2

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review