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Massively parallel assessment of human variants with base editor screens

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Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing

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CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank

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Design and analysis of CRISPR–Cas experiments

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CRISPR–Act3.0 for highly efficient multiplexed gene activation in plants

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Efficient in vivo genome editing prevents hypertrophic cardiomyopathy in mice

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Prime editing: advances and therapeutic applications

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Web-based design and analysis tools for CRISPR base editing

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Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG

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CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

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CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement

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Base editing correction of hypertrophic cardiomyopathy in human cardiomyocytes and humanized mice

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Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases

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Mimicking natural polymorphism in eIF 4E by CRISPR ‐Cas9 base editing is associated with resistance to potyviruses

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In vivo base editing restores sensory transduction and transiently improves auditory function in a mouse model of recessive deafness

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CRISPR–Cas: a tool for cancer research and therapeutics

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Precision genome editing using cytosine and adenine base editors in mammalian cells

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A catalogue of biochemically diverse CRISPR-Cas9 orthologs

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Perspectives on the Application of Genome-Editing Technologies in Crop Breeding

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CRISPR/Cas: Advances, Limitations, and Applications for Precision Cancer Research

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CRISPR-Cas9/Cas12a biotechnology and application in bacteria

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Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing

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Current Status and Challenges of DNA Base Editing Tools

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Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids

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Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice

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High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells

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Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era

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RNA‐based medicine: from molecular mechanisms to therapy

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Genome Engineering in Rice Using Cas9 Variants that Recognize NG PAM Sequences

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The Caenorhabditis elegans Transgenic Toolbox

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Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain

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High-efficiency and multiplex adenine base editing in plants using new TadA variants

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Base editing rescue of spinal muscular atrophy in cells and in mice

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CRISPR–Cas applications in agriculture and plant research

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Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes

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CRISPR-Based Genome Editing for Nutrient Enrichment in Crops: A Promising Approach Toward Global Food Security

Kumar, D., Yadav, A., Ahmad, R., Dwivedi, U. N., & Yadav, K. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.932859 · Cited by 139

Improved plant cytosine base editors with high editing activity, purity, and specificity

Ren, Q., Sretenovic, S., Liu, G., Zhong, Z., Wang, J., Huang, L., Tang, X., Guo, Y., Liu, L., Wu, Y., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13635 · Cited by 131

Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos

Chen, L., Hong, M., Luan, C., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., et al. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01821-9 · Cited by 135

An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis

Hino, T., Omura, S. N., Nakagawa, R., Togashi, T., Takeda, S. N., Hiramoto, T., Tasaka, S., Hirano, H., Tokuyama, T., Uosaki, H., et al. (2023) · Cell · doi:10.1016/j.cell.2023.08.031 · Cited by 131

Versatile detection with CRISPR/Cas system from applications to challenges

Wu, H., Chen, X., Zhang, M., Wang, X., Chen, Y., Qian, C., Wu, J. F., & Xu, J. (2020) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2020.116150 · Cited by 130

An engineered ScCas9 with broad PAM range and high specificity and activity

Chatterjee, P., Jakimo, N., Lee, J., Amrani, N., Rodríguez, T., Koseki, S., Tysinger, E., Qing, R., Hao, S., Sontheimer, E. J., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0517-0 · Cited by 131

Prediction of efficiencies for diverse prime editing systems in multiple cell types

Yu, G., Kim, H. K., Park, J., Kwak, H., Cheong, Y., Kim, D., Kim, J.-Y., Kim, J., & Kim, H. H. (2023) · Cell · doi:10.1016/j.cell.2023.03.034 · Cited by 128

Genome editing in large animals: current status and future prospects

Zhao, J., Lai, L., Ji, W., & Zhou, Q. (2019) · National Science Review · doi:10.1093/nsr/nwz013 · Cited by 129

Dead Cas Systems: Types, Principles, and Applications

Brezgin, S., Kostyusheva, A., Kostyushev, D. S., & Chulanov, V. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20236041 · Cited by 135

Massively parallel kinetic profiling of natural and engineered CRISPR nucleases

Jones, S. K., Hawkins, J. A., Johnson, N. V., Jung, C., Hu, K., Rybarski, J. R., Chen, J. S., Doudna, J. A., Press, W. H., & Finkelstein, I. J. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0646-5 · Cited by 131

CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences

Karlson, C. K. S., Noor, S. N. M., Nolte, N., & Tan, B. C. (2021) · Plants · doi:10.3390/plants10102055 · Cited by 139

The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato

Veillet, F., Chauvin, L., Kermarrec, M.-P., Sevestre, F., Merrer, M., Terret, Z., Szydlowski, N., Devaux, P., Gallois, J.-L., & Chauvin, J.-E. (2019) · Plant Cell Reports · doi:10.1007/s00299-019-02426-w · Cited by 124

Expanding the CRISPR Toolbox in Zebrafish for Studying Development and Disease

Liu, K., Petree, C., Requena, T., Varshney, P., & Varshney, G. K. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2019.00013 · Cited by 139

Base editing in plants: Current status and challenges

Bharat, S. S., Li, S., Li, J., Yan, L., & Xia, L. (2019) · The Crop Journal · doi:10.1016/j.cj.2019.10.002 · Cited by 126

Massively parallel base editing to map variant effects in human hematopoiesis

Martin-Rufino, J. D., Castano, N., Pang, M., Grody, E. I., Joubran, S. R., Caulier, A., Wahlster, L., Li, T., Qiu, X., Riera-Escandell, A. M., et al. (2023) · Cell · doi:10.1016/j.cell.2023.03.035 · Cited by 124

A Cas9 with PAM recognition for adenine dinucleotides

Chatterjee, P., Lee, J., Nip, L., Koseki, S., Tysinger, E., Sontheimer, E. J., Jacobson, J. M., & Jakimo, N. (2020) · Nature Communications · doi:10.1038/s41467-020-16117-8 · Cited by 126

Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM

Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 186

Expanding the targeting space of Cas9

Mao, S. (2018) · Science · doi:10.1126/science.361.6408.1211-g

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020) · Science · doi:10.1126/science.aba8853 · Cited by 1,233

Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation

Omura, S. N., Nakagawa, R., Kajimoto, S., Okazaki, S., Ishiguro, S., Mori, H., Onishi, K., Kashiwakura, Y., Hiramoto, T., Horinaka, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71626-2 · Cited by 2

Functional Characterization and Improved Engineering of the PAM-flexible Cas9 Variant, SpG-Cas9

Limbasia, S. (2023) · Texas Digital Library (University of Texas) · doi:10.26153/tsw/60656