Acute postprandial changes in leucine metabolism as assessed with an intrinsically labeled milk protein

Boirie, Y.; Gachon, P.; Corny, S.; Fauquant, J.; Maubois, J. L.; Beaufrere, B.

doi:10.1152/ajpendo.1996.271.6.e1083

Cited by 174Muscle metabolism and nutritionClinical Nutrition and GastroenterologyAnimal Nutrition and Physiology

Acute postprandial changes in leucine metabolism as assessed with an intrinsically labeled milk protein

Boirie, Y., Gachon, P., Corny, S., Fauquant, J., Maubois, J. L., Beaufrere, B.·1996·American Journal of Physiology-Endocrinology and Metabolism

DOI 10.1152/ajpendo.1996.271.6.e1083

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Abstract

Mechanisms of protein gain during protein feeding have been investigated using a combination of oral and intravenous labeled leucine in healthy young men. The oral labeled leucine was administered as a free oral tracer ([13C]- or [2H3]leucine) added to unlabeled whey protein or as whey protein intrinsically labeled with L-[1-13C]leucine. When the oral tracer was free leucine, it appeared in the plasma more rapidly than the unlabeled leucine derived from the whey protein, and this resulted in an artifactual 88% decrease of protein breakdown. When the oral tracer was protein bound, protein breakdown did not change significantly after the meal. In contrast, nonoxidative leucine disposal (i.e., protein synthesis) was stimulated by 63% by the meal. In conclusion, 1) an intrinsically labeled protein is more appropriate than an oral free tracer to study postprandial leucine kinetics under non-steady-state conditions and 2) protein gain after a single whey protein meal results solely from an increased protein synthesis with no modification of protein breakdown.

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Different types of proteins (e.g., casein, whey, and soy) are digested at different rates, which may affect whole body catabolism and anabolism and acute stimulation of muscle protein synthesis.
“For example, different types of proteins (e.g., casein, whey, and soy) are digested at different rates, which may affect whole body catabolism and anabolism and acute stimulation of muscle protein synthesis (MPS) [91–96].”
From ISSN exercise & sports nutrition review update: research & recommendations (2018) · cites this paper as [94]

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Time-dependent regulation of postprandial muscle protein synthesis rates after milk protein ingestion in young men
van Vliet, S., Beals, J. W., Holwerda, A. M., Emmons, R. S., Goessens, J. P. B., Paluska, S. A., Lisio, M. D., van Loon, L. J. C., & Burd, N. A. (2019) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00608.2019 · Cited by 24 · see citing works

Authors: Boirie, Y., Gachon, P., Corny, S., Fauquant, J., Maubois, J. L., & Beaufrere, B.
Affiliations: Laboratoire de Nutrition Humaine, Universite Clermont Auvergne,Clermont-Ferrand, France.
Université Clermont Auvergne (FR)
Venue: American Journal of Physiology-Endocrinology and Metabolism
Publisher: American Physiological Society
Published: 1996-12-01
Volume: 271
Issue: 6
Pages: E1083-E1091
ISSN: 0193-1849 (ISSN-L)
1522-1555
DOI: 10.1152/ajpendo.1996.271.6.e1083
OpenAlex: W1945646895
Cited by: 174
References: 12
Citations by year: 2026: 1 · 2025: 4 · 2024: 2 · 2023: 8 · 2022: 5 · 2021: 8 · 2020: 6 · 2019: 10 · 2018: 5 · 2017: 9 · 2016: 12 · 2015: 10 · 2014: 8 · 2013: 11 · 2012: 6
FWCI: 1.58
Type: journal article
Language: en
Open access: closed
Locations: American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
PubMed (publishedVersion)
HAL (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Health Sciences › Nursing › Nutrition and Dietetics › Clinical Nutrition and Gastroenterology
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords: leucine metabolism, protein degradation, whey protein, protein gain, leucine kinetics, non-steady state
MeSH terms: Administration, Oral, Adult, Carbon Radioisotopes, Humans, Injections, Intravenous, Leucine, Male, Milk Proteins, Tritium, Postprandial Period

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Acute postprandial changes in leucine metabolism as assessed with an intrinsically labeled milk protein

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