Cadaver validation of skeletal muscle measurement by magnetic resonance imaging and computerized tomography
Abstract
Magnetic resonance imaging (MRI) and computerized tomography (CT) are promising reference methods for quantifying whole body and regional skeletal muscle mass. Earlier MRI and CT validation studies used data-acquisition techniques and data-analysis procedures now outdated, evaluated anatomic rather than adipose tissue-free skeletal muscle (ATFSM), studied only the relatively large thigh, or found unduly large estimation errors. The aim of the present study was to compare arm and leg ATFSM cross-sectional area estimates (cm2) by using standard MRI and CT acquisition and image-analysis methods with corresponding cadaver estimates. A second objective was to validate MRI and CT measurements of adipose tissue embedded within muscle (interstitial adipose tissue) and surrounding muscle (subcutaneous adipose tissue). ATFSM area (n = 119) by MRI [38.9 +/- 22.3 (SD) cm2], CT (39.7 +/- 22.8 cm2), and cadaver (39.5 +/- 23.0 cm2) were not different (P > 0.001), and both MRI and CT estimates of ATFSM were highly correlated with corresponding cadaver values [MRI: r = 0.99, SE of estimate (SEE) 3.9 cm2, P < 0.001; and CT: r = 0.99, SEE = 3.8 cm2, P < 0.001]. Similarly good results were observed between MRI- and CT-measured vs. cadaver-measured interstitial and subcutaneous adipose tissue. For MRI-ATFSM the intraobserver correlation for duplicate measurements in vivo was 0. 99 [SEE = 8.7 cm2 (2.9%), P < 0.001]. These findings strongly support the use of MRI and CT as reference methods for app…
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Pooling the spectrum of methods for knee extensor mass/size and strength was done due to their high validity and reliability minimizing selection bias.
“We pooled the aforementioned spectrum of methods due to their high validity and reliability minimizing the selection bias (63–66).”
From Nonuniform loss of muscle strength and atrophy during bed rest: a systematic review (2021) · cites this paper as [64]
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- Authors
- Mitsiopoulos, N., Baumgartner, R. N., Heymsfield, S. B., Lyons, W., Gallagher, D., & Ross, R.
- Affiliations
- Queen's University (CA)
- University of New Mexico (US)
- Columbia University (US)
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 1998-07-01
- Volume
- 85
- Issue
- 1
- Pages
- 115-122
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2161349877
- Cited by
- 1,522
- References
- 3
- Citations by year
- 2026: 54 · 2025: 75 · 2024: 92 · 2023: 97 · 2022: 129 · 2021: 128 · 2020: 106 · 2019: 110 · 2018: 101 · 2017: 78 · 2016: 70 · 2015: 73 · 2014: 56 · 2013: 39 · 2012: 42
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Body Composition Measurement Techniques
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- skeletal muscle mass, adipose tissue, subcutaneous adipose tissue, cross-sectional area, magnetic resonance imaging, computed tomography, cadaver validation, measurement error
- MeSH terms
- Adipose Tissue, Aged, Aged, 80 and over, Body Composition, Cadaver, Humans, Image Processing, Computer-Assisted, Leg, Magnetic Resonance Imaging, Middle Aged, Obesity, Tomography, X-Ray Computed, Reproducibility of Results, Muscle, Skeletal
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