Cited by 214Neuroscience and Neuropharmacology ResearchProtease and Inhibitor MechanismsGenetics and Neurodevelopmental Disorders

Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology

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Michaluk, Piotr, Wawrzyniak, Marcin, Alot, Przemyslaw, Szczot, Marcin, Wyrembek, Paulina, Mercik, Katarzyna, Medvedev, Nikolay, Wilczek, Ewa +8 more·2011·Journal of Cell Science

Abstract

An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn2+-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.

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Crossref · 10.1242/jcs.090852

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  • The weakening of thalamic inputs to FS INs is a novel role for MMP-9.

    “The weakening of thalamic inputs to FS INs is a novel role for MMP-9, which is typically associated with the growth and strengthening of excitatory synapses onto excitatory neurons (Kaliszewska et al., 2012; Lebida and Mozrzymas, 2016; Michaluk et al., 2011; Ganguly et al., 2013), but see (Romberg et al., 2013).”

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Authors
Michaluk, P., Wawrzyniak, M., Alot, P., Szczot, M., Wyrembek, P., Mercik, K., Medvedev, N., Wilczek, E., De Roo, M., Zuschratter, W., Muller, D., Wilczynski, G. M., Mozrzymas, J. W., Stewart, M. G., Kaczmarek, L., & Wlodarczyk, J.
Affiliations
  • Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
  • Wroclaw Medical University (PL)
  • The Open University (GB)
  • Department of Neuroscience, Faculty of Medicine, University of Geneva, 1 rue Michel-Servet, CH-1211 Geneva 4, Switzerland
  • Leibniz Institute for Neurobiology (DE)
  • Department of Molecular and Cellular Neurobiology, The Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland
Corresponding author
  • Michaluk, Piotr
  • Kaczmarek, Leszek
Venue
Journal of Cell Science
Published
2011-10-01
Volume
124
Issue
19
Pages
3369-3380
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
OpenAlex
W2054463474
Cited by
214
References
73
Citations by year
2026: 8 · 2025: 5 · 2024: 6 · 2023: 10 · 2022: 18 · 2021: 12 · 2020: 17 · 2019: 15 · 2018: 13 · 2017: 15 · 2016: 19 · 2015: 16 · 2014: 19 · 2013: 23 · 2012: 14
FWCI
7.95
Type
journal article
Language
en
Open access
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, other-oa)
  • HighWire Press Open Archive (submittedVersion)
  • Open Research Online (The Open University) (submittedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
  • Pfizer
  • Fundacja na rzecz Nauki Polskiej
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
MMP-9, dendritic spine morphology, hippocampal neurons, synaptic remodeling, miniature synaptic currents, neuroplasticity, dissociated neuronal cultures
MeSH terms
Animals, Cells, Cultured, Chromatography, Affinity, Hippocampus, Microscopy, Fluorescence, Nerve Tissue Proteins, Recombinant Proteins, Rats, Wistar, Presynaptic Terminals, Patch-Clamp Techniques, Integrin beta1, Excitatory Postsynaptic Potentials, Matrix Metalloproteinase 9, Tissue Culture Techniques, Cell Shape, Dendritic Spines, Rats, Rats, Transgenic, Enzyme Assays, Primary Cell Culture

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