Cited by 230Single-cell and spatial transcriptomicsGenomics and Phylogenetic StudiesGene expression and cancer classification

Single-cell transcriptome sequencing: recent advances and remaining challenges

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Liu, Serena, Trapnell, Cole·2016·F1000Research

Abstract

Single-cell RNA-sequencing methods are now robust and economically practical and are becoming a powerful tool for high-throughput, high-resolution transcriptomic analysis of cell states and dynamics. Single-cell approaches circumvent the averaging artifacts associated with traditional bulk population data, yielding new insights into the cellular diversity underlying superficially homogeneous populations. Thus far, single-cell RNA-sequencing has already shown great effectiveness in unraveling complex cell populations, reconstructing developmental trajectories, and modeling transcriptional dynamics. Ongoing technical improvements to single-cell RNA-sequencing throughput and sensitivity, the development of more sophisticated analytical frameworks for single-cell data, and an increasing array of complementary single-cell assays all promise to expand the usefulness and potential applications of single-cell transcriptomic profiling.

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  • Current single-cell techniques suffer from low sensitivity and dropouts, making it challenging to reliably quantify low-abundance transcripts.

    “In addition, current single-cell techniques suffer from low sensitivity and dropouts; thus, it remains challenging to reliably quantify low-abundance transcripts (15, 53).”

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Authors
Liu, S., & Trapnell, C.
Affiliations
University of Washington (US)
Corresponding author
Trapnell, Cole
Venue
F1000Research
Published
2016-02-17
Volume
5
Pages
182
ISSN
2046-1402 (ISSN-L)
OpenAlex
W2288153033
Cited by
230
References
110
Citations by year
2026: 14 · 2025: 12 · 2024: 14 · 2023: 22 · 2022: 13 · 2021: 21 · 2020: 35 · 2019: 27 · 2018: 32 · 2017: 34 · 2016: 6
FWCI
2.99
Type
journal article
Language
en
Open access
Locations
  • F1000Research (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (1258485)
  • Damon Runyon Cancer Research Foundation
  • National Institutes of Health (NIH DP2)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (DP2HD088158)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
Keywords
single-cell RNA sequencing, single-cell transcriptomics, cellular heterogeneity, cell state dynamics, developmental trajectories, transcriptional dynamics, single-cell data analysis, single cell testing

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