Cited by 142Probiotics and Fermented FoodsGut microbiota and healthMicrobial Metabolites in Food Biotechnology

Probiotics as Complementary Treatment for Metabolic Disorders

Open access
Le Barz, Mélanie, Anhê, Fernando F., Varin, Thibaut V., Desjardins, Yves, Levy, Emile, Roy, Denis, Urdaci, Maria C., Marette, André·2015·Diabetes & Metabolism Journal

Abstract

Recent studies have demonstrated beneficial effects of specific probiotics on alleviating obesity-related disorders.Here we aimed to identify probiotics with potential antiobesity activity among 88 lactic acid bacterial strains via in vitro screening assays, and a Lactobacillus plantarum strain K21 was found to harbor abilities required for hydrolyzing bile salt, reducing cholesterol, and inhibiting the accumulation of lipid in 3T3-L1 preadipocytes.Furthermore, effects of K21 on diet-induced obese (DIO) mice were examined.Male C57Bl/6J mice received a normal diet, high-fat diet (HFD), or HFD with K21 administration (10 9 CFU in 0.2 mL PBS/day) for eight weeks.Supplementation of K21, but not placebo, appeared to alleviate body weight gain and epididymal fat mass accumulation, reduce plasma leptin levels, decrease cholesterol and triglyceride levels, and mitigate liver damage in DIO mice.Moreover, the hepatic expression of peroxisome proliferator-activated receptor-𝛾 (PPAR-𝛾) related to adipogenesis was significantly downregulated in DIO mice by K21 intervention.We also found that K21 supplementation strengthens intestinal permeability and modulates the amount of Lactobacillus spp., Bifidobacterium spp., and Clostridium perfringens in the cecal contents of DIO mice.In conclusion, our results suggest that dietary intake of K21 protects against the onset of HFD-induced obesity through multiple mechanisms of action.

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  • The effects of probiotic and prebiotic supplementation on metabolic health have been inconsistent due to the choice of probiotic strain, formulation, outcome of interest, and duration of intervention.

    “Although the effects of probiotic and prebiotic supplementation on metabolic health have been examined in previous studies, the results are inconsistent due to the choice of probiotic strain, formulation of the probiotic, outcome of interest, and duration of the intervention [12,13,14].”

    From Effects of Regular Kefir Consumption on Gut Microbiota in Patients with Metabolic Syndrome: A Parallel-Group, Randomized, Controlled Study (2019) · cites this paper as [13]

    The Conclusions report inconclusive probiotic results and attribute difficulty comparing studies to differences in protocols, including treatment duration. The paper also discusses strain-specific effects, product formulations, and differing metabolic outcomes. Its discussion of prebiotics does not establish that prebiotic supplementation has similarly inconsistent results for these reasons. (LLM, conf=0.88)

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Research works printed but not linked: 3

  1. [7]Hill C Guarner F Reid G Gibson GR Merenstein DJ Pot B Morelli L Canani RB Flint HJ Salminen S Calder PC Sanders ME Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic Nat Rev Gastroenterol Hepatol 2014 11 506 514 24912386
  2. [20]Oksaharju A Kooistra T Kleemann R van Duyvenvoorde W Miettinen M Lappalainen J Lindstedt KA Kovanen PT Korpela R Kekkonen RA Effects of probiotic Lactobacillus rhamnosus GG and Propionibacterium freudenreichii ssp. shermanii JS supplementation on intestinal and systemic markers of inflammation in ApoE*3Leiden mice consuming a high-fat diet Br J Nutr 2013 110 77 85 23211714
  3. [44]Smith PM Howitt MR Panikov N Michaud M Gallini CA Bohlooly YM Glickman JN Garrett WS The microbial metabolites, short-chain fatty acids, regulate colonic Treg cell homeostasis Science 2013 341 569 573 23828891 PMC3807819

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Authors
Le Barz, M., Anhê, F. F., Varin, T. V., Desjardins, Y., Levy, E., Roy, D., Urdaci, M. C., & Marette, A.
Affiliations
  • Department of Medicine, Faculty of Medicine, Cardiology Axis of the Québec Heart and Lung Institute, Québec, QC, Canada.
  • Institut universitaire de cardiologie et de pneumologie de Québec (CA)
  • Université Laval (CA)
  • Centre Hospitalier Universitaire Sainte-Justine (CA)
  • University of Bordeaux, UMR 5248, CBMN, Bordeaux, France.
Corresponding author
Marette, André
Venue
Diabetes & Metabolism Journal
Published
2015
Volume
39
Issue
4
Pages
291
ISSN
  • 2233-6079 (ISSN-L)
  • 2233-6087
OpenAlex
W2167419149
Cited by
142
References
61
Citations by year
2026: 8 · 2025: 18 · 2024: 10 · 2023: 9 · 2022: 14 · 2021: 7 · 2020: 15 · 2019: 14 · 2018: 14 · 2017: 15 · 2016: 15 · 2015: 3
FWCI
2.01
Type
journal article
Language
en
Update notices
erratum (2015-10-22)
Open access
Locations
  • Diabetes & Metabolism Journal (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
Keywords
probiotics, Lactobacillus plantarum, anti-obesity, diet-induced obesity, high-fat diet, bile salt hydrolysis, cholesterol lowering, leptin, hepatic steatosis, PPARγ, intestinal permeability, gut microbiota, 3T3-L1 preadipocytes, metabolic disorders

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