The effects of dietary restriction on oxidative stress in rodents
Open accessAbstract
Oxidative stress is observed during aging and in numerous age-related diseases. Dietary restriction (DR) is a regimen that protects against disease and extends life span in multiple species. However, it is unknown how DR mediates its protective effects. One prominent and consistent effect of DR in a number of systems is the ability to reduce oxidative stress and damage. The purpose of this review is to comprehensively examine the hypothesis that dietary restriction reduces oxidative stress in rodents by decreasing reactive oxygen species (ROS) production and increasing antioxidant enzyme activity, leading to an overall reduction of oxidative damage to macromolecules. The literature reveals that the effects of DR on oxidative stress are complex and likely influenced by a variety of factors, including sex, species, tissue examined, types of ROS and antioxidant enzymes examined, and duration of DR. Here we present a comprehensive review of the existing literature on the effect of DR on mitochondrial ROS generation, antioxidant enzymes, and oxidative damage. In a majority of studies, dietary restriction had little effect on mitochondrial ROS production or antioxidant activity. On the other hand, DR decreased oxidative damage in the majority of cases. Although the effects of DR on endogenous antioxidants are mixed, we find that glutathione levels are the most likely antioxidant to be increased by dietary restriction, which supports the emerging redox-stress hypothesis of aging.
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The effects of caloric restriction on mitochondrial function are not consistent across studies or across tissues.
“However, this is not consistent across studies or across tissues (Hancock et al., 2011; Lanza et al., 2012; Walsh et al., 2014).”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Walsh et al., 2014
Significant reduction in ROS production with caloric restriction is more likely if the duration of the caloric restriction exceeds 20 months.
“Of note, significant reduction in ROS production was more likely if the duration of the caloric restriction exceeded 20 months.”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Walsh et al., 2014
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Ricketts, W. G., Birchenall‐Sparks, M. C., Hardwick, J. P., & Richardson, A. G. (1985) · Journal of Cellular Physiology · doi:10.1002/jcp.1041250319 · Cited by 45 · see citing works
Rahman, M. M., Halade, G. V., Bhattacharya, A., & Fernandes, G. (2009) · Oxidative Medicine and Cellular Longevity · doi:10.4161/oxim.2.5.9579 · Cited by 43 · see citing works
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Sergi, D., Naumovski, N., Heilbronn, L. K., Abeywardena, M., O’Callaghan, N., Lionetti, L., & Luscombe-Marsh, N. (2019) · Frontiers in Physiology · doi:10.3389/fphys.2019.00532 · Cited by 347 · see citing works
“However, this is not consistent across studies or across tissues (Hancock et al., 2011; Lanza et al., 2012; Walsh et al., 2014).”
Cytoplasmic and Mitochondrial NADPH-Coupled Redox Systems in the Regulation of Aging
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Danger signals in liver injury and restoration of homeostasis
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Arias-Cavieres, A., Adasme, T., Sánchez, G., Muñoz, P., & Hidalgo, C. (2017) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2017.00111 · Cited by 70 · see citing works
Beneficial effects of dietary restriction in aging brain
Hadem, I. K. H., Majaw, T., Kharbuli, B., & Sharma, R. (2019) · Journal of Chemical Neuroanatomy · doi:10.1016/j.jchemneu.2017.10.001 · Cited by 59 · see citing works
Dietary Restriction and Neuroinflammation: A Potential Mechanistic Link
Bok, E., Jo, M., Lee, S., Lee, B.-R., Kim, J., & Kim, H.-J. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20030464 · Cited by 53 · see citing works
Effects of Sleep Deprivation on Mice Bone Marrow and Spleen B Lymphopoiesis
Lungato, L., Nogueira‐Pedro, A., Carvalho Dias, C., Paredes‐Gamero, E. J., Tufik, S., & D'Almeida, V. (2016) · Journal of Cellular Physiology · doi:10.1002/jcp.25231 · Cited by 18 · see citing works
Mulvey, L., Sands, W. A., Salin, K., Carr, A. E., & Selman, C. (2017) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2016.09.001 · Cited by 16 · see citing works
- Authors
- Walsh, M. E., Shi, Y., & Van Remmen, H.
- Affiliations
- The University of Texas at San Antonio Health Science Center (US)
- ORCID
- Shi, Yun
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 66
- Pages
- 88-99
- ISSN
- 0891-5849 (ISSN-L)
- PMC
- PMC4017324
- OpenAlex
- W2001107975
- Cited by
- 181
- References
- 232
- Citations by year
- 2026: 4 · 2025: 14 · 2024: 21 · 2023: 13 · 2022: 10 · 2021: 22 · 2020: 21 · 2019: 16 · 2018: 18 · 2017: 14 · 2016: 8 · 2015: 10 · 2014: 8 · 2013: 2
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- M.E.W (T32AG021890)
- National Institutes of Health – National Institute on Aging (P01AG20591)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- dietary restriction, oxidative stress, reactive oxygen species, antioxidant enzymes, mitochondrial reactive oxygen species, oxidative damage, glutathione, aging, longevity, sex differences, rodents
- MeSH terms
- Aging, Animals, Antioxidants, Catalase, Glutathione Peroxidase, Humans, Oxidation-Reduction, Superoxide Dismutase, Reactive Oxygen Species, Oxidative Stress, Caloric Restriction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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