Immune Outcomes of Paradoxical Sleep Deprivation on Cellular Distribution in Naive and Lipopolysaccharide-Stimulated Mice
Abstract
Background/Aims: Several lines of evidence indicate that sleep loss imposes significant consequences on the host defense system, including changes in cell number, activity and distribution. However, it is not clear whether cellular alterations after sleep deprivation are caused by redistribution to immune organs or by death of these cells or how the response to a nonspecific immune activator would be affected. Therefore, the aim of this study was to assess the leukocyte distribution after paradoxical sleep deprivation (PSD) in saline- and lipopolysaccharide-treated mice. Methods: Adult inbred mice were paradoxical sleep deprived (72 h), whereas the controls were kept in their home cages. After PSD, both groups received an injection of either saline or lipopolysaccharide (LPS; 1 or 5 µg/animal, intraperitoneally), 2 h prior to the collection of blood, spleen, lymph nodes and peritoneal wash. Isolated cells were then designated to differential leukocyte count (blood) and flow cytometry analysis of immune cell subsets (immune sites). Results: The data revealed that PSD caused a significant reduction of circulating lymphocytes and a general decrease in all cellular subsets of spleen, mainly T and B cells. However, no alteration in response of PSD was found on other immune sites, such as lymph nodes and peritoneum. Of note, immune cell distribution in response to in vivo LPS stimulation remained unchanged after PSD. Conclusions: Our study provided original evidence concerning the immune outcomes of PSD, indicating that cellular decrease caused by PSD is not restricted to circulation, but also to immune sites. Taken together, our results could help shed light on the physiological mechanisms of leukocyte trafficking.
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In rats undergoing prolonged sleep restriction for 21 consecutive days, a decrease in total leukocyte counts normalized after 24 h of recovery sleep, but did not normalize after recovery sleep following a REM sleep deprivation protocol over 4 consecutive days.
“In rats undergoing prolonged sleep restriction for 21 consecutive days, a decrease in total leukocyte counts normalized after 24 h of recovery sleep, but did not normalize after recovery sleep following a REM sleep deprivation protocol over 4 consecutive days (596).”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [596]
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- Authors
- Zager, A., Ruiz, F. S., Tufik, S., & Andersen, M. L.
- Affiliations
- Universidade Federal de São Paulo (BR)
- Venue
- Neuroimmunomodulation
- Publisher
- S. Karger AG
- Published
- 2012
- Volume
- 19
- Issue
- 2
- Pages
- 79-87
- ISSN
- 1021-7401 (ISSN-L)
- 1423-0216
- OpenAlex
- W2009202072
- Cited by
- 24
- References
- 35
- Citations by year
- 2025: 3 · 2024: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- NeuroImmunoModulation (publishedVersion)
- PubMed (publishedVersion)
- UNIFESP Institutional Repository (Universidade Federal de São Paulo) (submittedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Keywords
- REM sleep deprivation, sleep deprivation, immune cell distribution, leukocyte migration, lipopolysaccharide, lymphocyte depletion, T cells, B cells, spleen, lymph nodes, peritoneal lavage, innate immune response, mice
- MeSH terms
- Animals, Immunity, Cellular, Leukocyte Count, Leukocytes, Lipopolysaccharides, Male, Mice, Inbred C57BL, Sleep Deprivation, Sleep, REM, Mice
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