Characterization of development and artemisinin biosynthesis in self-pollinated Artemisia annua plants

Alejos-Gonzalez, Fatima; Qu, Guosheng; Zhou, Li-Li; Saravitz, Carole H.; Shurtleff, Janet L.; Xie, De-Yu

doi:10.1007/s00425-011-1430-z

Cited by 31Plant biochemistry and biosynthesisSesquiterpenes and Asteraceae StudiesPlant tissue culture and regeneration

Characterization of development and artemisinin biosynthesis in self-pollinated Artemisia annua plants

Alejos-Gonzalez, Fatima, Qu, Guosheng, Zhou, Li-Li, Saravitz, Carole H., Shurtleff, Janet L., Xie, De-Yu·2011·Planta

DOI 10.1007/s00425-011-1430-z

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Abstract

Artemisia annua L. is the only natural resource that produces artemisinin (Qinghaosu), an endoperoxide sesquiterpene lactone used in the artemisinin-combination therapy of malaria. The cross-hybridization properties of A. annua do not favor studying artemisinin biosynthesis. To overcome this problem, in this study, we report on selection of self-pollinated A. annua plants and characterize their development and artemisinin biosynthesis. Self-pollinated F2 plants selected were grown under optimized growth conditions, consisting of long day (16 h of light) and short day (9 h of light) exposures in a phytotron. The life cycles of these plants were approximately 3 months long, and final heights of 30-35 cm were achieved. The leaves on the main stems exhibited obvious morphological changes, from indented single leaves to odd, pinnately compound leaves. Leaves and flowers formed glandular and T-shaped trichomes on their surfaces. The glandular trichome densities increased from the bottom to the top leaves. High performance liquid chromatography-mass spectrometry-based metabolic profiling analyses showed that leaves, flowers, and young seedlings of F2 plants produced artemisinin. In leaves, the levels of artemisinin increased from the bottom to the top of the plants, showing a positive correlation to the density increase of glandular trichomes. RT-PCR analysis showed that progeny of self-pollinated plants expressed the amorpha-4, 11-diene synthase (ADS) and cytochrome P450 monooxygenase 71 AV1 (CYP71AV1) genes, which are involved in artemisinin biosynthesis in leaves and flowers. The use of self-pollinated A. annua plants will be a valuable approach to the study of artemisinin biosynthesis.

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Exploiting self-pollination for inbreeding of A. annua resulted in stable and relatively high artemisinin producing lines.
“Exploiting self-pollination for inbreeding of A. annua resulted in stable and relatively high artemisinin producing lines (Alejos-Gonzalez et al. 2011).”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Alejos-Gonzalez et al. 2011

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Ferreira, J. F. D. S. (1994) · Purdue e-Pubs (Purdue University System)
Artemisinin (Qinghaosu) Production in Artemisia annua
Ferreira, J., Simon, J. E., & Janick, J. (1995) · HortScience · doi:10.21273/hortsci.30.4.888b
Increased artemisinin production by promoting glandular secretory trichome formation and reconstructing the artemisinin biosynthetic pathway in Artemisia annua
Li, Y., Qin, W., Liu, H., Chen, T., Yan, X., He, W., Peng, B., Shao, J., Fu, X., Li, L., et al. (2023) · Horticulture Research · doi:10.1093/hr/uhad055 · Cited by 21 · see citing works

Authors: Alejos-Gonzalez, F., Qu, G., Zhou, L.-L., Saravitz, C. H., Shurtleff, J. L., & Xie, D.-Y.
Affiliations: North Carolina State University (US)
Corresponding author: Xie, De-Yu
ORCID: Saravitz, Carole H.
Xie, De-Yu
Venue: Planta
Publisher: Springer Science and Business Media LLC
Published: 2011-05-26
Volume: 234
Issue: 4
Pages: 685-697
ISSN: 0032-0935 (ISSN-L)
1432-2048
DOI: 10.1007/s00425-011-1430-z
OpenAlex: W2068461509
Cited by: 31
References: 42
Citations by year: 2026: 4 · 2025: 1 · 2023: 5 · 2022: 3 · 2021: 2 · 2019: 1 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 3
FWCI: 0.52
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Planta (publishedVersion)
PubMed (publishedVersion)
Funders: North Carolina Biotechnology Center
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
Keywords: Artemisia annua, artemisinin biosynthesis, self-pollination, F2 plants, plant development, glandular trichomes, leaf morphology, trichome density, amorpha-4,11-diene synthase, CYP71AV1, sesquiterpene lactones, metabolite profiling
MeSH terms: Antimalarials, Chromatography, High Pressure Liquid, Cytochrome P-450 Enzyme System, Lactones, Plant Proteins, Plants, Medicinal, Time Factors, Plant Leaves, Alkyl and Aryl Transferases, Spectrometry, Mass, Electrospray Ionization, Artemisia annua, Flowers, Seedlings, Artemisinins, Pollination, Self-Fertilization

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Characterization of development and artemisinin biosynthesis in self-pollinated Artemisia annua plants

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