Cited by 500Neuroinflammation and Neurodegeneration MechanismsTryptophan and brain disordersStress Responses and Cortisol

Peripheral administration of lipopolysaccharide induces the expression of cytokine transcripts in the brain and pituitary of mice

Layé, Sophie, Parnet, Patricia, Goujon, Emmanuelle, Dantzer, Robert·1994·Molecular Brain Research

Abstract

The reverse transcription polymerase chain reaction (RT-PCR) was used to assess the induction of mRNA of the proinflammatory cytokines IL-1 beta, IL-6 and TNF alpha in the spleen, pituitary, hypothalamus and hippocampus of mice after an intraperitoneal injection of lipopolysaccharide (LPS, 10 micrograms/mouse). The kinetics of cytokine gene expression induced by peripheral LPS in the pituitary and brain structures were different from that observed in the spleen. For IL-1 beta the dose-response curve was also measured and also found to be different. These results support the idea that one pathway by which peripheral immune stimuli affect brain functions includes local synthesis of proinflammatory cytokines in certain brain structures.

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Crossref · 10.1016/0169-328x(94)90197-x

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Authors
Layé, S., Parnet, P., Goujon, E., & Dantzer, R.
Affiliations
Inserm (FR)
Corresponding author
Dantzer, Robert
Venue
Molecular Brain Research
Publisher
Elsevier BV
Published
1994-11
Volume
27
Issue
1
Pages
157-162
ISSN
0169-328X (ISSN-L)
OpenAlex
W2057119181
Cited by
500
References
0
Citations by year
2026: 3 · 2025: 2 · 2024: 10 · 2023: 7 · 2022: 10 · 2021: 16 · 2020: 16 · 2019: 19 · 2018: 16 · 2017: 8 · 2016: 23 · 2015: 11 · 2014: 20 · 2013: 21 · 2012: 14
FWCI
9.86
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
Keywords
lipopolysaccharide, inflammatory cytokines, cytokine gene expression, brain, pituitary gland, hypothalamus, hippocampus, IL-1β, interleukin-6, TNF-α, neuroimmune interactions, spleen, dose-response relationship
MeSH terms
Animals, Base Sequence, beta 2-Microglobulin, Brain, Dose-Response Relationship, Drug, Gene Expression Regulation, Hippocampus, Hypothalamus, Inflammation, Interleukin-1, Kinetics, Lipopolysaccharides, Male, Molecular Sequence Data, Nerve Tissue Proteins, Pituitary Gland, Spleen, Tumor Necrosis Factor-alpha, Interleukin-6, Mice

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