Peripheral administration of lipopolysaccharide induces the expression of cytokine transcripts in the brain and pituitary of mice
Abstract
The reverse transcription polymerase chain reaction (RT-PCR) was used to assess the induction of mRNA of the proinflammatory cytokines IL-1 beta, IL-6 and TNF alpha in the spleen, pituitary, hypothalamus and hippocampus of mice after an intraperitoneal injection of lipopolysaccharide (LPS, 10 micrograms/mouse). The kinetics of cytokine gene expression induced by peripheral LPS in the pituitary and brain structures were different from that observed in the spleen. For IL-1 beta the dose-response curve was also measured and also found to be different. These results support the idea that one pathway by which peripheral immune stimuli affect brain functions includes local synthesis of proinflammatory cytokines in certain brain structures.
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- Authors
- Layé, S., Parnet, P., Goujon, E., & Dantzer, R.
- Affiliations
- Inserm (FR)
- Corresponding author
- Dantzer, Robert
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 1994-11
- Volume
- 27
- Issue
- 1
- Pages
- 157-162
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2057119181
- Cited by
- 500
- References
- 0
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 10 · 2023: 7 · 2022: 10 · 2021: 16 · 2020: 16 · 2019: 19 · 2018: 16 · 2017: 8 · 2016: 23 · 2015: 11 · 2014: 20 · 2013: 21 · 2012: 14
- FWCI
- 9.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Keywords
- lipopolysaccharide, inflammatory cytokines, cytokine gene expression, brain, pituitary gland, hypothalamus, hippocampus, IL-1β, interleukin-6, TNF-α, neuroimmune interactions, spleen, dose-response relationship
- MeSH terms
- Animals, Base Sequence, beta 2-Microglobulin, Brain, Dose-Response Relationship, Drug, Gene Expression Regulation, Hippocampus, Hypothalamus, Inflammation, Interleukin-1, Kinetics, Lipopolysaccharides, Male, Molecular Sequence Data, Nerve Tissue Proteins, Pituitary Gland, Spleen, Tumor Necrosis Factor-alpha, Interleukin-6, Mice
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