From Human Pluripotent Stem Cells to Cortical Circuits

Cerebral organoids model human brain development and microcephaly

Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372 · see citing works

Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling

Chambers, S. M., Fasano, C. A., Papapetrou, E. P., Tomishima, M., Sadelain, M., & Studer, L. (2009) · Nature Biotechnology · doi:10.1038/nbt.1529 · Cited by 3,620 · see citing works

Organogenesis in a dish: Modeling development and disease using organoid technologies

Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931 · see citing works

Zika Virus Associated with Microcephaly

Mlakar, J., Korva, M., Tul, N., Popović, M., Poljšak-Prijatelj, M., Mraz, J., Kolenc, M., Resman Rus, K., Vesnaver Vipotnik, T., Fabjan Vodušek, V., et al. (2016) · New England Journal of Medicine · doi:10.1056/nejmoa1600651 · Cited by 2,884 · see citing works

The Glial Nature of Embryonic and Adult Neural Stem Cells

Kriegstein, A., & Alvarez-Buylla, A. (2009) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.051508.135600 · Cited by 2,475 · see citing works

Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases

Noctor, S. C., Martínez-Cerdeño, V., Ivic, L., & Kriegstein, A. R. (2004) · Nature Neuroscience · doi:10.1038/nn1172 · Cited by 2,229 · see citing works

The cell biology of neurogenesis

Götz, M., & Huttner, W. B. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1739 · Cited by 2,130 · see citing works

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., Yao, B., Hamersky, G. R., Jacob, F., Zhong, C., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.032 · Cited by 2,127 · see citing works

Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture

Paşca, A. M., Sloan, S. A., Clarke, L. E., Tian, Y., Makinson, C. D., Huber, N., Kim, C. H., Park, J.-Y., O'Rourke, N. A., Nguyen, K. D., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3415 · Cited by 1,609 · see citing works

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

Eiraku, M., Watanabe, K., Matsuo-Takasaki, M., Kawada, M., Yonemura, S., Matsumura, M., Wataya, T., Nishiyama, A., Muguruma, K., & Sasai, Y. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.09.002 · Cited by 1,522 · see citing works

Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture

Ying, Q.-L., Stavridis, M., Griffiths, D., Li, M., & Smith, A. (2003) · Nature Biotechnology · doi:10.1038/nbt780 · Cited by 1,504 · see citing works

Regulation of Cerebral Cortical Size by Control of Cell Cycle Exit in Neural Precursors

Chenn, A., & Walsh, C. A. (2002) · Science · doi:10.1126/science.1074192 · Cited by 1,431 · see citing works

Extraordinary neoteny of synaptic spines in the human prefrontal cortex

Petanjek, Z., Judaš, M., Šimić, G., Rašin, M. R., Uylings, H. B. M., Rakic, P., & Kostović, I. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1105108108 · Cited by 1,411 · see citing works

Neurogenic radial glia in the outer subventricular zone of human neocortex

Hansen, D. V., Lui, J. H., Parker, P. R. L., & Kriegstein, A. R. (2010) · Nature · doi:10.1038/nature08845 · Cited by 1,388 · see citing works

Assembly of functionally integrated human forebrain spheroids

Birey, F., Andersen, J., Makinson, C. D., Islam, S., Wei, W., Huber, N., Fan, H. C., Metzler, K. R. C., Panagiotakos, G., Thom, N., et al. (2017) · Nature · doi:10.1038/nature22330 · Cited by 1,365 · see citing works

Development and Evolution of the Human Neocortex

Lui, J. H., Hansen, D. V., & Kriegstein, A. R. (2011) · Cell · doi:10.1016/j.cell.2011.06.030 · Cited by 1,342 · see citing works

A Model for Neural Development and Treatment of Rett Syndrome Using Human Induced Pluripotent Stem Cells

Marchetto, M. C. N., Carromeu, C., Acab, A., Yu, D., Yeo, G. W., Mu, Y., Chen, G., Gage, F. H., & Muotri, A. R. (2010) · Cell · doi:10.1016/j.cell.2010.10.016 · Cited by 1,329 · see citing works

Cell diversity and network dynamics in photosensitive human brain organoids

Quadrato, G., Nguyen, T., Macosko, E. Z., Sherwood, J. L., Min Yang, S., Berger, D. R., Maria, N., Scholvin, J., Goldman, M., Kinney, J. P., et al. (2017) · Nature · doi:10.1038/nature22047 · Cited by 1,321 · see citing works

Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth

Tang, H., Hammack, C., Ogden, S. C., Wen, Z., Qian, X., Li, Y., Yao, B., Shin, J., Zhang, F., Lee, E. M., et al. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.02.016 · Cited by 1,292 · see citing works

Zika virus impairs growth in human neurospheres and brain organoids

Garcez, P. P., Loiola, E. C., Madeiro da Costa, R., Higa, L. M., Trindade, P., Delvecchio, R., Nascimento, J. M., Brindeiro, R., Tanuri, A., & Rehen, S. K. (2016) · Science · doi:10.1126/science.aaf6116 · Cited by 1,184 · see citing works

FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders

Mariani, J., Coppola, G., Zhang, P., Abyzov, A., Provini, L., Tomasini, L., Amenduni, M., Szekely, A., Palejev, D., Wilson, M., et al. (2015) · Cell · doi:10.1016/j.cell.2015.06.034 · Cited by 1,149 · see citing works

Human cerebral organoids recapitulate gene expression programs of fetal neocortex development

Camp, J. G., Badsha, F., Florio, M., Kanton, S., Gerber, T., Wilsch-Bräuninger, M., Lewitus, E., Sykes, A., Hevers, W., Lancaster, M., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1520760112 · Cited by 1,130 · see citing works

A small step for the cell, a giant leap for mankind: a hypothesis of neocortical expansion during evolution

Rakic, P. (1995) · Trends in Neurosciences · doi:10.1016/0166-2236(95)93934-p · Cited by 1,047 · see citing works

Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: A major site of neurogenesis

Haubensak, W. E., Attardo, A., Denk, W. T., & Huttner, W. Β. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0308600100 · Cited by 1,004 · see citing works

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

Kadoshima, T., Sakaguchi, H., Nakano, T., Soen, M., Ando, S., Eiraku, M., & Sasai, Y. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1315710110 · Cited by 999 · see citing works

Molecular Identity of Human Outer Radial Glia during Cortical Development

Pollen, A. A., Nowakowski, T. J., Chen, J., Retallack, H., Sandoval-Espinosa, C., Nicholas, C. R., Shuga, J., Liu, S. J., Oldham, M. C., Diaz, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.004 · Cited by 908 · see citing works

Molecular logic of neocortical projection neuron specification, development and diversity

Greig, L. C., Woodworth, M. B., Galazo, M. J., Padmanabhan, H., & Macklis, J. D. (2013) · Nature Reviews Neuroscience · doi:10.1038/nrn3586 · Cited by 863 · see citing works

OSVZ progenitors of human and ferret neocortex are epithelial-like and expand by integrin signaling

Fietz, S. A., Kelava, I., Vogt, J., Wilsch-Bräuninger, M., Stenzel, D., Fish, J. L., Corbeil, D., Riehn, A., Distler, W., Nitsch, R., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2553 · Cited by 830 · see citing works

Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses

Shi, Y., Kirwan, P., Smith, J., Robinson, H. P. C., & Livesey, F. J. (2012) · Nature Neuroscience · doi:10.1038/nn.3041 · Cited by 813 · see citing works

Fused cerebral organoids model interactions between brain regions

Bagley, J. A., Reumann, D., Bian, S., Lévi-Strauss, J., & Knoblich, J. A. (2017) · Nature Methods · doi:10.1038/nmeth.4304 · Cited by 790 · see citing works

Directed differentiation of telencephalic precursors from embryonic stem cells

Watanabe, K., Kamiya, D., Nishiyama, A., Katayama, T., Nozaki, S., Kawasaki, H., Watanabe, Y., Mizuseki, K., & Sasai, Y. (2005) · Nature Neuroscience · doi:10.1038/nn1402 · Cited by 784 · see citing works

Guided self-organization and cortical plate formation in human brain organoids

Lancaster, M. A., Corsini, N. S., Wolfinger, S., Gustafson, E. H., Phillips, A. W., Burkard, T. R., Otani, T., Livesey, F. J., & Knoblich, J. A. (2017) · Nature Biotechnology · doi:10.1038/nbt.3906 · Cited by 783 · see citing works

Direct Neural Fate Specification from Embryonic Stem Cells

Tropepe, V., Hitoshi, S., Sirard, C., Mak, T. W., Rossant, J., & van der Kooy, D. (2001) · Neuron · doi:10.1016/s0896-6273(01)00263-x · Cited by 757 · see citing works

The Cell Biology of Neurogenesis: Toward an Understanding of the Development and Evolution of the Neocortex

Taverna, E., Götz, M., & Huttner, W. Β. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101011-155801 · Cited by 737 · see citing works

Cell Cycle Dependence of Laminar Determination in Developing Neocortex

McConnell, S. K., & Kaznowski, C. E. (1991) · Science · doi:10.1126/science.1925583 · Cited by 712 · see citing works

De novo somatic mutations in components of the PI3K-AKT3-mTOR pathway cause hemimegalencephaly

Lee, J. H., Huynh, M., Silhavy, J. L., Kim, S., Dixon-Salazar, T., Heiberg, A., Scott, E., Bafna, V., Hill, K. J., Collazo, A., et al. (2012) · Nature Genetics · doi:10.1038/ng.2329 · Cited by 703 · see citing works

An intrinsic mechanism of corticogenesis from embryonic stem cells

Gaspard, N., Bouschet, T., Hourez, R., Dimidschstein, J., Naeije, G., van den Ameele, J., Espuny-Camacho, I., Herpoel, A., Passante, L., Schiffmann, S. N., et al. (2008) · Nature · doi:10.1038/nature07287 · Cited by 641 · see citing works

Neural induction, the default model and embryonic stem cells

Muñoz-Sanjuán, I., & Brivanlou, A. H. (2002) · Nature reviews. Neuroscience · doi:10.1038/nrn786 · Cited by 635 · see citing works

A Role for Intermediate Radial Glia in the Tangential Expansion of the Mammalian Cerebral Cortex

Reillo, I., Romero, C. D. J., García‐Cabezas, M. Á., & Borrell, V. (2010) · Cerebral Cortex · doi:10.1093/cercor/bhq238 · Cited by 631 · see citing works

The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells

Shen, Q., Wang, Y., Dimos, J. T., Fasano, C. A., Phoenix, T. N., Lemischka, I. R., Ivanova, N. B., Stifani, S., Morrisey, E. E., & Temple, S. (2006) · Nature Neuroscience · doi:10.1038/nn1694 · Cited by 625 · see citing works

Directed Differentiation and Functional Maturation of Cortical Interneurons from Human Embryonic Stem Cells

Maroof, A. M., Keros, S., Tyson, J. A., Ying, S.-W., Ganat, Y. M., Merkle, F. T., Liu, B., Goulburn, A., Stanley, E. G., Elefanty, A. G., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.04.008 · Cited by 596 · see citing works

Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome

Paşca, S. P., Portmann, T., Voineagu, I., Yazawa, M., Shcheglovitov, A., Paşca, A. M., Cord, B., Palmer, T. D., Chikahisa, S., Nishino, S., et al. (2011) · Nature Medicine · doi:10.1038/nm.2576 · Cited by 585 · see citing works

Pyramidal Neurons Derived from Human Pluripotent Stem Cells Integrate Efficiently into Mouse Brain Circuits In Vivo

Espuny-Camacho, I., Michelsen, K. A., Gall, D., Linaro, D., Hasche, A., Bonnefont, J., Bali, C., Orduz, D., Bilheu, A., Herpoel, A., et al. (2013) · Neuron · doi:10.1016/j.neuron.2012.12.011 · Cited by 568 · see citing works

Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia

Bershteyn, M., Nowakowski, T. J., Pollen, A. A., Di Lullo, E., Nene, A., Wynshaw-Boris, A., & Kriegstein, A. R. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2016.12.007 · Cited by 566 · see citing works

Cdk4/CyclinD1 Overexpression in Neural Stem Cells Shortens G1, Delays Neurogenesis, and Promotes the Generation and Expansion of Basal Progenitors

Lange, C. M., Huttner, W. Β., & Calegari, F. (2009) · Cell stem cell · doi:10.1016/j.stem.2009.05.026 · Cited by 565 · see citing works

The Evolution of the Brain, the Human Nature of Cortical Circuits, and Intellectual Creativity

DeFelipe, J. (2011) · Frontiers in Neuroanatomy · doi:10.3389/fnana.2011.00029 · Cited by 527 · see citing works

Modeling human cortical development in vitro using induced pluripotent stem cells

Mariani, J., Simonini, M. V., Palejev, D., Tomasini, L., Coppola, G., Szekely, A. M., Horvath, T. L., & Vaccarino, F. M. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1202944109 · Cited by 526 · see citing works

Evolution of the Human Nervous System Function, Structure, and Development

Sousa, A. M. M., Meyer, K. A., Santpere, G., Gulden, F. O., & Sestan, N. (2017) · Cell · doi:10.1016/j.cell.2017.06.036 · Cited by 517 · see citing works

Functional Maturation of hPSC-Derived Forebrain Interneurons Requires an Extended Timeline and Mimics Human Neural Development

Nicholas, C. R., Chen, J., Tang, Y., Southwell, D. G., Chalmers, N., Vogt, D. L., Arnold, C. M., Chen, Y.-J. J., Stanley, E. G., Elefanty, A. G., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.04.005 · Cited by 513 · see citing works

Zika virus cell tropism in the developing human brain and inhibition by azithromycin

Retallack, H., Di Lullo, E., Arias, C., Knopp, K. A., Laurie, M. T., Sandoval-Espinosa, C., Mancia Leon, W. R., Krencik, R., Ullian, E. M., Spatazza, J., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1618029113 · Cited by 506 · see citing works

Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin

Pera, M. F., Andrade, J., Houssami, S., Reubinoff, B. E., Trounson, A. O., Stanley, E. G., Oostwaard, D. W., & Mummery, C. L. (2004) · Journal of Cell Science · doi:10.1242/jcs.00970 · Cited by 471 · see citing works

SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients

Shcheglovitov, A., Shcheglovitova, O., Yazawa, M., Portmann, T., Shu, R., Sebastiano, V., Krawisz, A., Froehlich, W., Bernstein, J. A., Hallmayer, J. F., et al. (2013) · Nature · doi:10.1038/nature12618 · Cited by 470 · see citing works

Altered proliferation and networks in neural cells derived from idiopathic autistic individuals

Marchetto, M. C., Belinson, H., Tian, Y., Freitas, B. C., Fu, C., Vadodaria, K. C., Beltrao-Braga, P. C., Trujillo, C. A., Mendes, A. P. D., Padmanabhan, K., et al. (2017) · Molecular Psychiatry · doi:10.1038/mp.2016.95 · Cited by 466 · see citing works

Early Steps in the Development of the Forebrain

Wilson, S. W., & Houart, C. (2004) · Developmental Cell · doi:10.1016/s1534-5807(04)00027-9 · Cited by 460 · see citing works

Pluripotent stem cells in regenerative medicine: challenges and recent progress

Tabar, V. S., & Studer, L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3563 · Cited by 458 · see citing works

Inhibition of SRGAP2 function by its human-specific paralogs induces neoteny during spine maturation

Charrier, C., Joshi, K., Coutinho-Budd, J., Kim, J.-E., Lambert, N., de Marchena, J., Jin, W.-L., Vanderhaeghen, P., Ghosh, A., Sassa, T., et al. (2012) · Cell · doi:10.1016/j.cell.2012.03.034 · Cited by 454 · see citing works

Induction of Expansion and Folding in Human Cerebral Organoids

Li, Y., Muffat, J., Omer, A., Bosch, I., Lancaster, M. A., Sur, M., Gehrke, L., Knoblich, J. A., & Jaenisch, R. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2016.11.017 · Cited by 436 · see citing works

The hope and the hype of organoid research

Huch, M., Knoblich, J. A., Lutolf, M. P., & Martinez-Arias, A. (2017) · Development · doi:10.1242/dev.150201 · Cited by 409 · see citing works

Transcriptional neoteny in the human brain

Somel, M., Franz, H., Zheng, Y., Lorenc, A., Guo, S., Giger, T., Kelso, J. F., Nickel, B., Dannemann, M., Bahn, S., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0900544106 · Cited by 409 · see citing works

KCC2 Expression Promotes the Termination of Cortical Interneuron Migration in a Voltage-Sensitive Calcium-Dependent Manner

Bortone, D., & Polleux, F. (2009) · Neuron · doi:10.1016/j.neuron.2009.01.034 · Cited by 387 · see citing works

Restriction of Late Cerebral Cortical Progenitors to an Upper-Layer Fate

Frantz, G. D., & McConnell, S. K. (1996) · Neuron · doi:10.1016/s0896-6273(00)80280-9 · Cited by 356 · see citing works

2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size

Otani, T., Marchetto, M. C., Gage, F. H., Simons, B. D., & Livesey, F. J. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.03.003 · Cited by 353 · see citing works

CHD8 haploinsufficiency results in autistic-like phenotypes in mice

Katayama, Y., Nishiyama, M., Shoji, H., Ohkawa, Y., Kawamura, A., Sato, T., Suyama, M., Takumi, T., Miyakawa, T., & Nakayama, K. I. (2016) · Nature · doi:10.1038/nature19357 · Cited by 353 · see citing works

Fate-Restricted Neural Progenitors in the Mammalian Cerebral Cortex

Franco, S. J., Gil, C., Martínez‐Garay, I., Espinosa, A., Harkins-Perry, S. R., Ramos, C., & Müller, U. (2012) · Science · doi:10.1126/science.1223616 · Cited by 334 · see citing works

The outer subventricular zone and primate-specific cortical complexification

Dehay, C., Kennedy, H., & Kosik, K. S. (2015) · Neuron · doi:10.1016/j.neuron.2014.12.060 · Cited by 314 · see citing works

Induction and Dorsoventral Patterning of the Telencephalon

Wilson, S. W., & Rubenstein, J. L. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00171-9 · Cited by 314 · see citing works

Autism-associated SHANK3 haploinsufficiency causes <i>I</i> _h channelopathy in human neurons

Yi, F., Danko, T., Botelho, S. C., Patzke, C., Pak, C., Wernig, M., & Südhof, T. C. (2016) · Science · doi:10.1126/science.aaf2669 · Cited by 313 · see citing works

The promises and challenges of human brain organoids as models of neuropsychiatric disease

Quadrato, G., Brown, J., & Arlotta, P. (2016) · Nature Medicine · doi:10.1038/nm.4214 · Cited by 304 · see citing works

Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development

Mora‐Bermúdez, F., Badsha, F., Kanton, S., Camp, J. G., Vernot, B., Köhler, K., Voigt, B., Okita, K., Maričić, T., He, Z., et al. (2016) · eLife · doi:10.7554/elife.18683 · Cited by 289 · see citing works

Cdk5rap2 regulates centrosome function and chromosome segregation in neuronal progenitors

Lizarraga, S. B., Margossian, S. P., Harris, M. H., Campagna, D. R., Han, A., Blevins, S., Mudbhary, R., Barker, J. E., Walsh, C. A., & Fleming, M. D. (2010) · Development · doi:10.1242/dev.040410 · Cited by 270 · see citing works

An Organoid-Based Model of Cortical Development Identifies Non-Cell-Autonomous Defects in Wnt Signaling Contributing to Miller-Dieker Syndrome

Iefremova, V., Manikakis, G., Krefft, O., Jabali, A., Weynans, K., Wilkens, R., Marsoner, F., Brändl, B., Müller, F.-J., Koch, P., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.03.047 · Cited by 261 · see citing works

CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in cerebral organoids derived from iPS cells

Wang, P., Mokhtari, R., Pedrosa, E., Kirschenbaum, M. A., Bayrak, C., Zheng, D., & Lachman, H. M. (2017) · Molecular Autism · doi:10.1186/s13229-017-0124-1 · Cited by 255 · see citing works

Extension of cortical synaptic development distinguishes humans from chimpanzees and macaques

Liu, X., Somel, M., Tang, L., Yan, Z., Jiang, X., Guo, S., Yuan, Y., He, L., Oleksiak, A., Zhang, Y., et al. (2012) · Genome Research · doi:10.1101/gr.127324.111 · Cited by 251 · see citing works

Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells

Qi, Y., Zhang, X.-J., Renier, N., Wu, Z., Atkin, T., Sun, Z., Ozair, M. Z., Tchieu, J., Zimmer, B., Fattahi, F., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3777 · Cited by 248 · see citing works

Hallmarks of Alzheimer’s Disease in Stem-Cell-Derived Human Neurons Transplanted into Mouse Brain

Espuny-Camacho, I., Arranz, A. M., Fiers, M., Snellinx, A., Ando, K., Munck, S., Bonnefont, J., Lambot, L., Corthout, N., Omodho, L., et al. (2017) · Neuron · doi:10.1016/j.neuron.2017.02.001 · Cited by 244 · see citing works

Genetic causes of microcephaly and lessons for neuronal development

Gilmore, E. C., & Walsh, C. A. (2012) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.89 · Cited by 244 · see citing works

Modeling a Genetic Risk for Schizophrenia in iPSCs and Mice Reveals Neural Stem Cell Deficits Associated with Adherens Junctions and Polarity

Yoon, K.-J., Nguyen, H. N., Ursini, G., Zhang, F., Kim, N.-S., Wen, Z., Makri, G., Nauen, D., Shin, J. H., Park, Y., et al. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.05.003 · Cited by 240 · see citing works

CPAP promotes timely cilium disassembly to maintain neural progenitor pool

Gabriel, E., Wason, A., Ramani, A., Gooi, L. M., Keller, P., Pozniakovsky, A., Poser, I., Noack, F., Telugu, N. S., Calegari, F., et al. (2016) · The EMBO Journal · doi:10.15252/embj.201593679 · Cited by 238 · see citing works

Using brain organoids to understand Zika virus-induced microcephaly

Qian, X., Nguyen, H. N., Jacob, F., Song, H., & Ming, G. (2017) · Development · doi:10.1242/dev.140707 · Cited by 238 · see citing works

Mechanisms and pathways of growth failure in primordial dwarfism.Preprint

Klingseisen, A., & Jackson, A. P. (2011) · Genes & Development · doi:10.1101/gad.169037 · Cited by 237 · see citing works

Increased LIS1 expression affects human and mouse brain development

Bi, W., Sapir, T., Shchelochkov, O. A., Zhang, F., Withers, M. A., Hunter, J. V., Levy, T., Shinder, V., Peiffer, D. A., Gunderson, K. L., et al. (2009) · Nature Genetics · doi:10.1038/ng.302 · Cited by 223 · see citing works

MECP2 disorders: from the clinic to mice and back

Lombardi, L. M., Baker, S. A., & Zoghbi, H. Y. (2015) · Journal of Clinical Investigation · doi:10.1172/jci78167 · Cited by 221 · see citing works

Neurogenesis at the Brain–Cerebrospinal Fluid Interface

Lehtinen, M. K., & Walsh, C. A. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-092910-154026 · Cited by 213 · see citing works

Xenotransplanted human cortical neurons reveal species-specific development and functional integration into mouse visual circuits

Linaro, D., Vermaercke, B., Iwata, R., Ramaswamy, A., Davis, B. A., Boubakar, L., Libé-Philippot, B., Bilheu, A., Bruyne, L. D., Gall, D., et al. (2019) · doi:10.1101/626218 · Cited by 210 · see citing works

Cortical area and species differences in dendritic spine morphology

Benavides-Piccione, R., Ballesteros-Yáñez, I., DeFelipe, J., & Yuste, R. (2002) · Journal of Neurocytology · doi:10.1023/a:1024134312173 · Cited by 207 · see citing works

Fgf10 Regulates Transition Period of Cortical Stem Cell Differentiation to Radial Glia Controlling Generation of Neurons and Basal Progenitors

Sahara, S., & O’Leary, D. D. M. (2009) · Neuron · doi:10.1016/j.neuron.2009.06.006 · Cited by 206 · see citing works

Zika-Virus-Encoded NS2A Disrupts Mammalian Cortical Neurogenesis by Degrading Adherens Junction Proteins

Yoon, K., Song, G., Qian, X., Pan, J., Xu, D., Rho, H.-S., Kim, N.-S., Habela, C. W., Zheng, L., Jacob, F., et al. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.07.014 · Cited by 206 · see citing works

Mutations in mouse Aspm (abnormal spindle-like microcephaly associated) cause not only microcephaly but also major defects in the germline

Pulvers, J. N., Bryk, J., Fish, J. L., Wilsch‐Bräuninger, M., Arai, Y., Schreier, D., Naumann, R., Helppi, J., Habermann, B. H., Vogt, J., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1010494107 · Cited by 194 · see citing works

Integration of GABAergic interneurons into cortical cell assemblies: lessons from embryos and adults

Bartolini, G., Ciceri, G., & Marín, O. (2013) · Neuron · doi:10.1016/j.neuron.2013.08.014 · Cited by 185 · see citing works

Interneurons from Embryonic Development to Cell-Based Therapy

Southwell, D. G., Nicholas, C. R., Basbaum, A. I., Stryker, M. P., Kriegstein, A. R., Rubenstein, J. L. R., & Álvarez-Buylla, A. (2014) · Science · doi:10.1126/science.1240622 · Cited by 185 · see citing works

Cortical neurogenesis in the absence of centrioles

Insolera, R., Bazzi, H., Shao, W., Anderson, K. V., & Shi, S. (2014) · Nature Neuroscience · doi:10.1038/nn.3831 · Cited by 180 · see citing works

Human uniqueness: genome interactions with environment, behaviour and culture

Varki, A. P., Geschwind, D. H., & Eichler, E. E. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2428 · Cited by 168 · see citing works

Integration of Telencephalic Wnt and Hedgehog Signaling Center Activities by Foxg1

Danesin, C., Peres, J., Johansson, M., Snowden, V., Cording, A., Papalopulu, N., & Houart, C. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.03.007 · Cited by 164 · see citing works

Self-formation of layered neural structures in three-dimensional culture of ES cells

Eiraku, M., & Sasai, Y. (2012) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2012.02.005 · Cited by 162 · see citing works

Human induced pluripotent stem cells for modelling neurodevelopmental disorders

Ardhanareeswaran, K., Mariani, J., Coppola, G., Abyzov, A. S., & Vaccarino, F. M. (2017) · Nature Reviews Neurology · doi:10.1038/nrneurol.2017.45 · Cited by 160 · see citing works

Area-Specific Reestablishment of Damaged Circuits in the Adult Cerebral Cortex by Cortical Neurons Derived from Mouse Embryonic Stem Cells

Michelsen, K. A., Acosta-Verdugo, S., Benoit-Marand, M., Espuny-Camacho, I., Gaspard, N., Saha, B., Gaillard, A., & Vanderhaeghen, P. (2015) · Neuron · doi:10.1016/j.neuron.2015.02.001 · Cited by 138 · see citing works

Genetic Changes Shaping the Human Brain

Bae, B.-I., Jayaraman, D., & Walsh, C. A. (2015) · Developmental Cell · doi:10.1016/j.devcel.2015.01.035 · Cited by 135 · see citing works

Human neoteny revisited: The case of synaptic plasticity

Bufill, E., Agustí, J., & Blesa, R. (2011) · American Journal of Human Biology · doi:10.1002/ajhb.21225 · Cited by 130 · see citing works

Cortical interneuron specification: the juncture of genes, time and geometry

Bandler, R. C., Mayer, C., & Fishell, G. (2017) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2016.10.003 · Cited by 125 · see citing works

Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate

Srikanth, P., Han, K., Callahan, D. G., Makovkina, E., Muratore, C., Lalli, M. A., Zhou, H., Boyd, J. D., Kosik, K. S., Selkoe, D. J., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.07.061 · Cited by 118 · see citing works

Altered neuronal network and rescue in a human MECP2 duplication model

Nageshappa, S., Carromeu, C., Trujillo, C. A., Mesci, P., Espuny-Camacho, I., Pasciuto, E., Vanderhaeghen, P., Verfaillie, C. M., Raitano, S., Kumar, A., et al. (2016) · Molecular Psychiatry · doi:10.1038/mp.2015.128 · Cited by 117 · see citing works

Intermediate Progenitor Cohorts Differentially Generate Cortical Layers and Require Tbr2 for Timely Acquisition of Neuronal Subtype Identity

Mihalas, A. B., Elsen, G. E., Bedogni, F., Daza, R. A. M., Ramos-Laguna, K. A., Arnold, S. J., & Hevner, R. F. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.05.072 · Cited by 113 · see citing works

Glial process elongation and branching in the developing murine neocortex: A qualitative and quantitative immunohistochemical analysis

Takahashi, T., Misson, J., & Caviness, V. S. (1990) · The Journal of Comparative Neurology · doi:10.1002/cne.903020103 · Cited by 108 · see citing works

Assessing similarity to primary tissue and cortical layer identity in induced pluripotent stem cell-derived cortical neurons through single-cell transcriptomics

Handel, A. E., Chintawar, S., Lalic, T., Whiteley, E. S., Vowles, J., Giustacchini, A., Argoud, K., Sopp, P., Nakanishi, M., Bowden, R. J., et al. (2016) · Human Molecular Genetics · doi:10.1093/hmg/ddv637 · Cited by 104 · see citing works

Dynamic behaviour of human neuroepithelial cells in the developing forebrain

Subramanian, L., Bershteyn, M., Paredes, M. F., & Kriegstein, A. R. (2017) · Nature Communications · doi:10.1038/ncomms14167 · Cited by 94 · see citing works

LIS1 functions in normal development and disease

Reiner, O., & Sapir, T. (2013) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2013.08.001 · Cited by 94 · see citing works

Alteration in basal and depolarization induced transcriptional network in iPSC derived neurons from Timothy syndrome

Tian, Y., Voineagu, I., Paşca, S. P., Won, H., Chandran, V., Horvath, S., Dolmetsch, R. E., & Geschwind, D. H. (2014) · Genome Medicine · doi:10.1186/s13073-014-0075-5 · Cited by 93 · see citing works

Transcriptomic Perspectives on Neocortical Structure, Development, Evolution, and Disease

Lein, E. S., Belgard, T. G., Hawrylycz, M., & Molnár, Z. (2017) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-070815-013858 · Cited by 92 · see citing works

Disruption of an Evolutionarily Novel Synaptic Expression Pattern in Autism

Liu, X., Han, D., Somel, M., Jiang, X., Hu, H., Guijarro, P., Zhang, N., Mitchell, A., Halene, T., Ely, J. J., et al. (2016) · PLOS Biology · doi:10.1371/journal.pbio.1002558 · Cited by 87 · see citing works

Timing temporal transitions during brain development

Rossi, A., Fernandes, V. M., & Desplan, C. (2016) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2016.11.010 · Cited by 74 · see citing works

Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations

Gouder, L., Vitrac, A., Goubran‐Botros, H., Danckaert, A., Tinévez, J.-Y., André-Leroux, G., Atanasova, E., Lemière, N., Biton, A., Leblond, C. S., et al. (2019) · Scientific Reports · doi:10.1038/s41598-018-36993-x · Cited by 66 · see citing works

Pluripotent stem cell-based disease modeling: current hurdles and future promise

Zeltner, N., & Studer, L. (2015) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2015.10.008 · Cited by 65 · see citing works

Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1

Garcez, P. P., Díaz-Alonso, J., Crespo‐Enríquez, I., Castro, D. S., Bell, D. M., & Guillemot, F. (2015) · Nature Communications · doi:10.1038/ncomms7474 · Cited by 62 · see citing works

Division modes and physical asymmetry in cerebral cortex progenitors

Delaunay, D., Kawaguchi, A., Dehay, C., & Matsuzaki, F. (2016) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2016.11.009 · Cited by 39 · see citing works

Laminar fate specification in the cerebral cortex

Gaspard, N., & Vanderhaeghen, P. (2011) · F1000 Biology Reports · doi:10.3410/b3-6 · Cited by 36 · see citing works

Novel and Robust Transplantation Reveals the Acquisition of Polarized Processes by Cortical Cells Derived from Mouse and Human Pluripotent Stem Cells

Nagashima, F., Suzuki, I., Shitamukai, A., Sakaguchi, H., Iwashita, M., Kobayashi, T., Toné, S., Toida, K., Vanderhaeghen, P., & Kosodo, Y. (2013) · Stem Cells and Development · doi:10.1089/scd.2013.0251 · Cited by 32 · see citing works

Modeling the autistic cell: iPSCs recapitulate developmental principles of syndromic and nonsyndromic ASD

Ben‐Reuven, L., & Reiner, O. (2016) · Development Growth & Differentiation · doi:10.1111/dgd.12280 · Cited by 26 · see citing works

Definitions and classification of malformations of cortical development: practical guidelines

Severino, M., Geraldo, A. F., Utz, N., Tortora, D., Pogledić, I., Klonowski, W., Triulzi, F. M., Arrigoni, F., Mankad, K., Leventer, R. J., et al. (2020) · Brain · doi:10.1093/brain/awaa174 · Cited by 312 · see citing works

Xenotransplanted human cortical neurons reveal species-specific development and functional integration into mouse visual circuits

Linaro, D., Vermaercke, B., Iwata, R., Ramaswamy, A., Davis, B. A., Boubakar, L., Libé-Philippot, B., Bilheu, A., Bruyne, L. D., Gall, D., et al. (2019) · doi:10.1101/626218 · Cited by 210 · see citing works

LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification

Garza, R., Atacho, D. A. M., Adami, A., Gerdes, P., Vinod, M., Hsieh, P., Karlsson, O., Horváth, V., Johansson, P. A., Pandiloski, N., et al. (2023) · Science Advances · doi:10.1126/sciadv.adh9543 · Cited by 83 · see citing works

Altered spinogenesis in iPSC-derived cortical neurons from patients with autism carrying de novo SHANK3 mutations

Gouder, L., Vitrac, A., Goubran‐Botros, H., Danckaert, A., Tinévez, J.-Y., André-Leroux, G., Atanasova, E., Lemière, N., Biton, A., Leblond, C. S., et al. (2019) · Scientific Reports · doi:10.1038/s41598-018-36993-x · Cited by 66 · see citing works

CROCCP2 acts as a human-specific modifier of cilia dynamics and mTOR signaling to promote expansion of cortical progenitors

Heurck, R. V., Bonnefont, J., Wojno, M., Suzuki, I., Velez-Bravo, F. D., Erkol, E., Nguyen, D. T., Herpoel, A., Bilheu, A., Beckers, S., et al. (2022) · Neuron · doi:10.1016/j.neuron.2022.10.018 · Cited by 51 · see citing works

Neuronal fate acquisition and specification: time for a change

Bonnefont, J., & Vanderhaeghen, P. (2021) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2020.12.006 · Cited by 47 · see citing works

Temporal regulation of neural diversity in Drosophila and vertebrates

El-Danaf, R. N., Rajesh, R., & Desplan, C. (2022) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2022.05.011 · Cited by 41 · see citing works

Human Pluripotent Stem Cell-Derived Astrocyte Functionality Compares Favorably with Primary Rat Astrocytes

Lendemeijer, B., Unkel, M., Smeenk, H., Mossink, B., Hijazi, S., Gordillo-Sampedro, S., Shpak, G., Slump, D. E., van den Hout, M. C. G. N., van IJcken, W. F. J., et al. (2024) · eNeuro · doi:10.1523/eneuro.0148-24.2024 · Cited by 28 · see citing works

A polarized FGF8 source specifies frontotemporal signatures in spatially oriented cell populations of cortical assembloids

Bosone, C., Castaldi, D., Burkard, T. R., Guzman, S. J., Wyatt, T., Cheroni, C., Caporale, N., Bajaj, S., Bagley, J. A., Li, C. V., et al. (2024) · Nature Methods · doi:10.1038/s41592-024-02412-5 · Cited by 28 · see citing works

Axonal branch patterning and neuronal shape diversity: roles in developmental circuit assembly

Hoersting, A.-K., & Schmucker, D. (2020) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2020.10.019 · Cited by 22 · see citing works

Group I mGluR-Mediated Activation of Martinotti Cells Inhibits Local Cortical Circuitry in Human Cortex

Kroon, T., Dawitz, J., Kramvis, I., Anink, J. J., Obermayer, J., Verhoog, M. B., Wilbers, R., Goriounova, N. A., Idema, S., Baayen, J. C., et al. (2019) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2019.00315 · Cited by 18 · see citing works

How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex

Pipicelli, F., Villalba, A., & Hippenmeyer, S. (2025) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2025.103046 · Cited by 9 · see citing works

Navigating Human Astrocyte Differentiation: Direct and Rapid One‐Step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network Development

Schuurmans, I. M. E., Mordelt, A., Guevara‐Ferrer, M., Linda, K., Duineveld, D. J., Puvogel, S., Hommersom, M. P., Scheefhals, N., Rahm, L., Dyke, E., et al. (2026) · Glia · doi:10.1002/glia.70202 · Cited by 4 · see citing works

Human adherent cortical organoids in a multiwell formatPreprint

van der Kroeg, M., Bansal, S., Unkel, M., Smeenk, H., Kushner, S. A., & de Vrij, F. M. S. (2024) · eLife · doi:10.7554/elife.98340.1 · Cited by 3 · see citing works

Human adherent cortical organoids in a multi-well format

van der Kroeg, M., Bansal, S., Unkel, M., Smeenk, H., Kushner, S. A., & de Vrij, F. M. S. (2026) · eLife · doi:10.7554/elife.98340.3 · Cited by 2 · see citing works

CROCCP2 acts as a human-specific modifier of cilia dynamics and mTOR signalling to promote expansion of cortical progenitorsPreprint

Heurck, R. V., Wojno, M., Suzuki, I., Velez-Bravo, F. D., Bonnefont, J., Erkol, E., Nguyen, D. T., Herpoel, A., Bilheu, A., Ledent, C. A., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.06.17.142976 · Cited by 2 · see citing works

Ontogeny of oscillatory slow-wave and neuronal population activity in human iPSC-3D cortical circuitsPreprint

Izsák, J., Theiss, S., & Illes, S. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.03.14.484311 · Cited by 2 · see citing works

Tracing cell lineages in the developing brain: Insights from mosaic analysis and clone-resolved transcriptomics

Varela-Martínez, I., Pipicelli, F., & Hippenmeyer, S. (2026) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2026.102487 · Cited by 1 · see citing works

Malformations of Cortical Development

Geraldo, A. F., Rossi, A., & Severino, M. (2021) · Springer eBooks · doi:10.1007/978-3-662-46258-4_6-1 · Cited by 2 · see citing works

Decoding the porcine developing spatial processing system and production of human entorhinal stellate cell-like cells by a direct programming approachPreprint

Bergmann, T., Liu, Y., Mogus, L., Lee, J., Pfisterer, U., Handfield, L.-F., Asenjo-Martinez, A., Vargas, I. L., Seemann, S. E., Lee, J. T. H., et al. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/738443

Sequenciamento completo do exoma no diagnóstico de defeitos do desenvolvimento cerebelar e da migração neuronal cerebral

Cabral, K. S. S. (2022) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/t.5.2022.tde-20072022-104745

Detailed protocol for the generation of polarized cortical assembloids (polCA)Preprint

Bosone, C., Barilà, S. E., Krenn, V., & Knoblich, J. A. (2024) · Research Square · doi:10.21203/rs.3.pex-2641/v1

Author response: Human adherent cortical organoids in a multi-well format

van der Kroeg, M., Bansal, S., Unkel, M., Smeenk, H., Kushner, S. A., & de Vrij, F. M. S. (2024) · eLife · doi:10.7554/elife.98340.1.sa4

eLife Assessment: Human adherent cortical organoids in a multi-well format

Konopka, G. (2024) · eLife · doi:10.7554/elife.98340.1.sa3

Reviewer #1 (Public Review): Human adherent cortical organoids in a multiwell format

(2024) · eLife · doi:10.7554/elife.98340.1.sa2

Reviewer #3 (Public Review): Human adherent cortical organoids in a multiwell format

(2024) · eLife · doi:10.7554/elife.98340.1.sa0

Reviewer #2 (Public Review): Human adherent cortical organoids in a multiwell format

(2024) · eLife · doi:10.7554/elife.98340.1.sa1

Human adherent cortical organoids in a multi-well formatPreprint

van der Kroeg, M., Bansal, S., Unkel, M., Smeenk, H., Kushner, S. A., & de Vrij, F. M. S. (2024) · eLife · doi:10.7554/elife.98340 · Cited by 1 · see citing works

Brain cell types and distribution

Kooijmans, R. N. (2024) · Elsevier eBooks · doi:10.1016/b978-0-12-820480-1.00198-4

Malformations of Cortical Development (MCDs)

Pooh, R. K. (2024) · Springer eBooks · doi:10.1007/978-3-031-70192-4_8

Methods

Kempermann, G. (2025) · doi:10.1093/med/9780197519233.003.0007

Human adherent cortical organoids in a multiwell formatPreprint

van der Kroeg, M., Bansal, S., Unkel, M., Smeenk, H., Kushner, S. A., & de Vrij, F. M. S. (2026) · eLife · doi:10.7554/elife.98340.2

Analyse histologique et fonctionnelle du développement de précurseurs neuraux dérivés de cellules souches pluripotentes induites humaines greffés dans le cortex de la souris

Raïs, C. (2019) · theses.fr (ABES) · doi:10.70675/e853aef3za211z4be9z822fzfb9846d925a7

Microcephaly-causing WDR81 mutation alters endosomal trafficking of EGFR and leads to reduced progenitor cell proliferation

Carpentieri, J. A. (2020) · theses.fr (ABES) · doi:10.70675/1e00c88fzf627z42c5za72ez7da656ac6044

Pluripotent Stem Cell Based Cultures to Study Key Aspects of Human Cerebral Cortex Development

Ehsaei, Z., Collo, G., & Taylor, V. (2018) · Institutional Research Information System (Università degli Studi di Brescia) · doi:10.4172/neuropsychiatry.1000511 · Cited by 1 · see citing works

Thinking out of the dish: what to learn about cortical development using pluripotent stem cells

van den Ameele, J., Tiberi, L., Vanderhaeghen, P., & Espuny-Camacho, I. (2014) · Trends in Neurosciences · doi:10.1016/j.tins.2014.03.005 · Cited by 110 · see citing works

Molecular Mechanisms Controlling the Development and Evolution of the Human Cerebral Cortex

Strano, A. (2019) · Apollo (University of Cambridge) · doi:10.17863/cam.48318

ISDN2012_0276: From pluripotent stem cells to cortical circuits

Vanderhaeghen, P. (2012) · International Journal of Developmental Neuroscience · doi:10.1016/j.ijdevneu.2012.10.098

Cortical neurogenesis from pluripotent stem cells: complexity emerging from simplicity

Anderson, S., & Vanderhaeghen, P. (2014) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2014.03.012 · Cited by 40 · see citing works