A wearable lab-on-a-patch platform with stretchable nanostructured biosensor for non-invasive immunodetection of biomarker in sweat
Abstract
Conformable, wearable biosensor-integrated systems are a promising approach to non-invasive and quantitative on-body detection of biomarkers in body fluids. However, realizing such a system has been slowed by the difficulty of fabricating a soft affinity-based biosensor patch capable of precise on-body fluid handling with minimal wearer intervention and a simple measurement protocol. Herein, we demonstrate a conformable, wearable lab-on-a-patch (LOP) platform composed of a stretchable, label-free, impedimetric biosensor and a stretchable microfluidic device for on-body detection of the hormone biomarker, cortisol. The all-in-one, stretchable microfluidic device can precisely collect and deliver sweat for cortisol quantitation and offers one-touch operation of reagent delivery for simultaneous electrochemical signal generation and washing. Three-dimensional nanostructuring of the Au working electrode enables the high sensitivity required to detect the pM-levels of cortisol in sweat. Our integrated LOP detected sweat cortisol quantitatively and accurately during exercise. This LOP will open a new horizon for non-invasive, highly sensitive, and quantitative on-body immunodetection for wearable personal diagnostics.
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- Authors
- Lee, H.-B., Meeseepong, M., Trung, T. Q., Kim, B.-Y., & Lee, N.-E.
- Affiliations
- Sungkyunkwan University (KR)
- Corresponding author
- Lee, Nae-Eung
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2020-05
- Volume
- 156
- Pages
- 112133
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W3010245527
- Cited by
- 153
- References
- 63
- Citations by year
- 2026: 12 · 2025: 17 · 2024: 24 · 2023: 33 · 2022: 29 · 2021: 28 · 2020: 10
- FWCI
- 7.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Basic Science Research Program (2019R1A6A1A03033215)
- Nano Material Technology Development Program (NRF-2016M3A7B4910554)
- National Research Foundation
- Ministry of Science, ICT, & Future Planning
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Sensor and Energy Harvesting Materials
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Keywords
- wearable biosensors, impedimetric biosensor, cortisol detection, sweat biomarkers, physical activity, non-invasive diagnosis, personal health monitoring
- MeSH terms
- Wearable Electronic Devices, Elasticity, Electrodes, Equipment Design, Humans, Hydrocortisone, Immunoassay, Sweat, Biosensing Techniques, Biomarkers, Microfluidic Analytical Techniques, Nanostructures, Lab-On-A-Chip Devices
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