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A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq
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Summary: Spermatogenesis requires intricate interactions between the germline and somatic cells. Within a given cross-section of a seminiferous tubule, multiple germ and somatic cell types co-occur. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. To address this challenge, we collected single-cell RNA sequencing data from ~35K cells from the adult mouse testis, and identified all known germ and somatic cells, as well as two unexpected somatic cell types. Our analysis revealed a continuous developmental trajectory of germ cells from spermatogonia to spermatids, and identified novel candidate transcriptional regulators at several transition points during differentiation. Focused analyses delineated four subtypes of spermatogonia and nine subtypes of Sertoli cells, the latter linked to histologically defined developmental stages over the seminiferous epithelial cycle. Overall, this high-resolution cellular atlas represents a community resource and foundation of knowledge to study germ cell development and in vivo gametogenesis.
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Metadata
- Authors
- Green, C. D., Ma, Q., Manske, G. L., Shami, A. N., Zheng, X., Marini, S., Moritz, L., Sultan, C., Gurczynski, S. J., Moore, B. B., Tallquist, M. D., Li, J. Z., & Hammoud, S. S.
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 46
- Issue
- 5
- Pages
- 651-667.e10
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2888403713
- Cited by
- 521
- References
- 125
- FWCI
- 36.15
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.