Cited by 203Neuroinflammation and Neurodegeneration MechanismsParkinson's Disease Mechanisms and TreatmentsNuclear Receptors and Signaling

Minocycline Reduces the Lipopolysaccharide-Induced Inflammatory Reaction, Peroxynitrite-Mediated Nitration of Proteins, Disruption of the Blood–Brain Barrier, and Damage in the Nigral Dopaminergic System

Open access
Tomás-Camardiel, Mayka, Rite, Inmaculada, Herrera, Antonio J, de Pablos, Rocio M, Cano, Josefina, Machado, Alberto, Venero, José L·2004·Neurobiology of Disease

Abstract

We have evaluated the potential neuroprotectant activity of minocycline in an animal model of Parkinson's disease induced by intranigral injection of lipopolysaccharide. Minocycline treatment was very effective in protecting number of nigral dopaminergic neurons and loss of reactive astrocytes at 7 days postlesion. Evaluation of microglia revealed that minocycline treatment highly prevented the lipopolysaccharide-induced activation of reactive microglia as visualized by OX-42 and OX-6 immunohistochemistry. Short-term RT-PCR analysis demonstrated that minocycline partially prevented the lipopolysaccharide-induced increases of mRNA levels for interleukin-1alpha and tumor necrosis factor-alpha. In addition, lipopolysaccharide highly induced protein nitration as seen by 3-nitrotyrosine immunoreactivity in the ventral mesencephalon. Minocycline treatment strongly diminished the extent of 3-nitrotyrosine immunoreactivity. We also found a direct correlation between location of IgG immunoreactivity-a marker of blood-brain barrier disruption-and neurodegenerative processes including death of nigral dopaminergic cells and reactive astrocytes. There was also a precise spatial correlation between disruption of blood-brain barrier and 3-nitrotyrosine immunoreactivity. We discuss potential involvement of lipopolysaccharide-induced formation of peroxynitrites and cytokines in the pathological events in substantia nigra in response to inflammation. If inflammation is proved to be involved in the ethiopathology of Parkinson's disease, our data support the use of minocycline in parkinsonian patients.

Integrity signals

Registry

Crossref · 10.1016/j.nbd.2004.01.010

Cites

79 of 79 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

1 of the 80 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Tomás-Camardiel, M., Rite, I., Herrera, A. J., de Pablos, R. M., Cano, J., Machado, A., & Venero, J. L.
Affiliations
Universidad de Sevilla (ES)
Venue
Neurobiology of Disease
Publisher
Elsevier BV
Published
2004-06
Volume
16
Issue
1
Pages
190-201
ISSN
0969-9961 (ISSN-L)
OpenAlex
W1966437242
Cited by
203
References
80
Citations by year
2026: 4 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 5 · 2020: 10 · 2019: 7 · 2018: 8 · 2017: 7 · 2016: 8 · 2015: 10 · 2014: 13 · 2013: 9 · 2012: 11
FWCI
10.17
Type
journal article
Language
en
Open access
Locations
  • Neurobiology of Disease (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministerio de Ciencia Tecnología y Telecomunicaciones (BFI 2001-3600)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
Keywords
minocycline, Parkinson's disease, lipopolysaccharide, neuroprotection, substantia nigra, dopaminergic neurons, peroxynitrite, protein nitration, nitrotyrosine, blood-brain barrier disruption, microglial activation, reactive astrocytes, interleukin-1 alpha, TNF-α, neuroinflammation
MeSH terms
Animals, Blood-Brain Barrier, Dopamine, Female, Inflammation, Lipopolysaccharides, Minocycline, Substantia Nigra, Rats, Wistar, Nitric Oxide Synthase, Peroxynitrous Acid, Rats, Nitric Oxide Synthase Type II

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review