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Cited by 21,607

The DOI matches its Crossref record.

A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Open access
Grynkiewicz, G, Poenie, M, Tsien, R Y·1985·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(19)83641-4
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Abstract

A new family of highly fluorescent indicators has been synthesized for biochemical studies of the physiological role of cytosolic free Ca2+. The compounds combine an 8-coordinate tetracarboxylate chelating site with stilbene chromophores. Incorporation of the ethylenic linkage of the stilbene into a heterocyclic ring enhances the quantum efficiency and photochemical stability of the fluorophore. Compared to their widely used predecessor, "quin2", the new dyes offer up to 30-fold brighter fluorescence, major changes in wavelength not just intensity upon Ca2+ binding, slightly lower affinities for Ca2+, slightly longer wavelengths of excitation, and considerably improved selectivity for Ca2+ over other divalent cations. These properties, particularly the wavelength sensitivity to Ca2+, should make these dyes the preferred fluorescent indicators for many intracellular applications, especially in single cells, adherent cell layers, or bulk tissues.

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Metadata

Authors
Grynkiewicz, G., Poenie, M., & Tsien, R. Y.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1985-03
Volume
260
Issue
6
Pages
3440-3450
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(19)83641-4
OpenAlex
W2141260882
Cited by
21,607
References
0
FWCI
228.30
Type
journal article
Language
en
Open access
hybrid

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.