The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Abstract
RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Here we show that 21-nucleotide siRNA duplexes specifically suppress expression of endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
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“The RNA-cleaving properties of Cas13a can be harnessed for post-transcriptional repression, with comparable efficiency to RNA interference (RNAi) methods of RNA silencing [123,124]”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [123]
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“This has been demonstrated to be the most powerful way to selectively reduce the intracellular concentration of proteins (18,19).”
In the citing paper
“RNA interference (RNAi) is an endogenous cellular process that triggers the degradation of particular RNA targets by double-stranded RNAs, result in the subsequent down-regulation of corresponding proteins.565–567”
From Recent advances in targeting the “undruggable” proteins: from drug discovery to clinical trials (2023) · cites this paper as [565]
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Metadata
- Authors
- Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- Max Planck Society (DE)
- Corresponding author
- Tuschl, Thomas
- Venue
- Nature
- Published
- 2001-05
- Volume
- 411
- Issue
- 6836
- Pages
- 494-498
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1611419331
- Cited by
- 9,456
- References
- 31
- Citations by year
- 2026: 130 · 2025: 145 · 2024: 151 · 2023: 141 · 2022: 218 · 2021: 201 · 2020: 196 · 2019: 232 · 2018: 195 · 2017: 224 · 2016: 242 · 2015: 287 · 2014: 359 · 2013: 401 · 2012: 486
- FWCI
- 210.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- RNA interference, siRNA, gene silencing, post-transcriptional regulation, mammalian cells
- MeSH terms
- Animals, Coleoptera, Cells, Cultured, Cnidaria, Drosophila, Genes, Genetic Techniques, HeLa Cells, Humans, Nuclear Proteins, Nucleic Acid Hybridization, RNA, Double-Stranded, RNA, Messenger, Vimentin, Genes, Reporter, Cell Cycle Proteins, COS Cells, Gene Silencing, Nuclear Matrix-Associated Proteins, Lamins, Antigens, Nuclear
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