Cited by 9,456RNA Interference and Gene DeliveryRNA Research and SplicingPlant Molecular Biology Research

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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

Elbashir, Sayda M., Harborth, Jens, Lendeckel, Winfried, Yalcin, Abdullah, Weber, Klaus, Tuschl, Thomas·2001·Nature

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Abstract

RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Here we show that 21-nucleotide siRNA duplexes specifically suppress expression of endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.

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Authors: Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T.
Affiliations: Max Planck Institute for Biophysical Chemistry (DE)
Max Planck Society (DE)
Corresponding author: Tuschl, Thomas
ORCID: Elbashir, Sayda M.
Harborth, Jens
Tuschl, Thomas
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2001-05
Volume: 411
Issue: 6836
Pages: 494-498
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/35078107
OpenAlex: W1611419331
Cited by: 9,456
References: 31
Citations by year: 2026: 130 · 2025: 145 · 2024: 151 · 2023: 141 · 2022: 218 · 2021: 201 · 2020: 196 · 2019: 232 · 2018: 195 · 2017: 224 · 2016: 242 · 2015: 287 · 2014: 359 · 2013: 401 · 2012: 486
FWCI: 210.46
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords: RNA interference, siRNA, gene silencing, post-transcriptional regulation, mammalian cells
MeSH terms: Animals, Coleoptera, Cells, Cultured, Cnidaria, Drosophila, Genes, Genetic Techniques, HeLa Cells, Humans, Nuclear Proteins, Nucleic Acid Hybridization, RNA, Double-Stranded, RNA, Messenger, Vimentin, Genes, Reporter, Cell Cycle Proteins, COS Cells, Gene Silencing, Nuclear Matrix-Associated Proteins, Lamins, Antigens, Nuclear

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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

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