Cited by 781Neuroinflammation and Neurodegeneration MechanismsNeurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology Research

Layer V cortical neurons require microglial support for survival during postnatal development

Ueno, Masaki, Fujita, Yuki, Tanaka, Tatsuhide, Nakamura, Yuka, Kikuta, Junichi, Ishii, Masaru, Yamashita, Toshihide·2013·Nature Neuroscience

Abstract

Neurons require trophic support during neural circuit formation; however, how the cellular milieu contributes to neuronal survival remains unclear. We found that layer V cortical neurons require support from microglia for survival during postnatal development. Specifically, we found that microglia accumulated close to the subcerebral and callosal projection axons in the postnatal brain. Inactivation of microglia by minocycline treatment or transient ablation of microglia in CD11b-DTR transgenic mice led to increased apoptosis, specifically in layer V subcerebral and callosal projection neurons. CX3CR1 in microglia was required for the survival of layer V neurons. Microglia consistently promoted the survival of cortical neurons in vitro. In addition, we identified microglia-derived IGF1 as a trophic factor that maintained neuronal survival. Our results highlight a neuron-glia interaction that is indispensable for network formation during a specific period in the developing brain.

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Crossref · 10.1038/nn.3358

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Authors
Ueno, M., Fujita, Y., Tanaka, T., Nakamura, Y., Kikuta, J., Ishii, M., & Yamashita, T.
Affiliations
  • Japan Science and Technology Agency (JP)
  • Asahikawa Medical University (JP)
  • Immunology Frontier Research Center, The University of Osaka (JP)
Corresponding author
  • Ueno, Masaki
  • Yamashita, Toshihide
Venue
Nature Neuroscience
Published
2013-03-24
Volume
16
Issue
5
Pages
543-551
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2015412022
Cited by
781
References
50
Citations by year
2026: 41 · 2025: 54 · 2024: 67 · 2023: 61 · 2022: 65 · 2021: 81 · 2020: 82 · 2019: 69 · 2018: 64 · 2017: 52 · 2016: 50 · 2015: 43 · 2014: 41 · 2013: 11
FWCI
26.72
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
microglia, neuronal survival, postnatal brain development, insulin-like growth factor I, trophic support, neuron-glia interactions, CX3CR1, apoptosis, minocycline, callosal projection neurons, microglial activation
MeSH terms
CX3C Chemokine Receptor 1, Animals, Animals, Newborn, Calcium-Binding Proteins, Cell Survival, Cells, Cultured, Diphtheria Toxin, Embryo, Mammalian, Enzyme Inhibitors, Humans, Insulin-Like Growth Factor I, Mice, Inbred C57BL, Mice, Transgenic, Microfilament Proteins, Nerve Tissue Proteins, Neurons, Visual Cortex, Apoptosis, Microglia, Gene Expression Regulation, Developmental, Coculture Techniques, Receptors, Chemokine, CD11b Antigen, Mice, Calcium-Calmodulin-Dependent Protein Kinase Type 2

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