Astrocytic Ca2+ signaling evoked by sensory stimulation in vivo
Abstract
Although astrocytes are the most abundant cell type in the brain, evidence for their activation during physiological sensory activity is lacking. Here we show that whisker stimulation evokes increases in astrocytic cytosolic calcium (Ca(2+)) within the barrel cortex of adult mice. Increases in astrocytic Ca(2+) were a function of the frequency of stimulation, occurred within several seconds and were inhibited by metabotropic glutamate receptor antagonists. To distinguish between synaptic input and output, local synaptic activity in cortical layer 2 was silenced by iontophoresis of AMPA and NMDA receptor antagonists. The antagonists did not reduce astrocytic Ca(2+) responses despite a marked reduction in excitatory postsynaptic currents in response to whisker stimulation. These findings indicate that astrocytes respond to synaptic input, by means of spillover or ectopic release of glutamate, and that increases in astrocytic Ca(2+) occur independently of postsynaptic excitatory activity.
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Astrocytes can detect synaptic activity via glutamatergic receptors.
“Astrocytes are capable of detecting synaptic activity through glutamatergic receptors [80].”
From ApoE, ApoE Receptors, and the Synapse in Alzheimer's Disease (2017) · cites this paper as [80]
Astrocytes are sensitive to changes in neural activity and respond with increases in intracellular calcium levels.
“In vivo imaging studies of astrocytes have shown that astrocytes are sensitive to changes in neural activity and respond with increases in intracellular calcium levels (Wang et al., 2006; Winship et al., 2007).”
From Adult neurogenesis, glia, and the extracellular matrix (2019) · cites this paper as Wang et al., 2006
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- Authors
- Wang, X., Lou, N., Xu, Q., Tian, G.-F., Peng, W. G., Han, X., Kang, J., Takano, T., & Nedergaard, M.
- Affiliations
- University of Rochester Medicine (US)
- New York Medical College (US)
- Corresponding author
- Nedergaard, Maiken
- Venue
- Nature Neuroscience
- Published
- 2006-05-14
- Volume
- 9
- Issue
- 6
- Pages
- 816-823
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W2026154459
- Cited by
- 685
- References
- 50
- Citations by year
- 2026: 13 · 2025: 30 · 2024: 15 · 2023: 25 · 2022: 38 · 2021: 24 · 2020: 27 · 2019: 24 · 2018: 31 · 2017: 30 · 2016: 26 · 2015: 37 · 2014: 47 · 2013: 51 · 2012: 52
- FWCI
- 16.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (NS38073, R01NS038073, NS 30007)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- astrocytes, calcium signaling, sensory stimulation, whisker stimulation, barrel cortex, metabotropic glutamate receptors, glutamate spillover, synaptic input, AMPA receptor antagonists, NMDA receptor antagonists, excitatory postsynaptic currents, astrocyte activation
- MeSH terms
- Afferent Pathways, Animals, Astrocytes, Calcium, Cell Communication, Female, Male, Mechanoreceptors, Synaptic Transmission, Physical Stimulation, Reaction Time, Somatosensory Cortex, Touch, Vibrissae, Up-Regulation, Receptors, N-Methyl-D-Aspartate, Receptors, AMPA, Receptors, Metabotropic Glutamate, Excitatory Amino Acid Antagonists, Excitatory Postsynaptic Potentials, Calcium Signaling, Mice
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