Cited by 685Neuroscience and Neuropharmacology ResearchNeuroinflammation and Neurodegeneration MechanismsNeural dynamics and brain function

Astrocytic Ca2+ signaling evoked by sensory stimulation in vivo

Wang, Xiaohai, Lou, Nanhong, Xu, Qiwu, Tian, Guo-Feng, Peng, Wei Guo, Han, Xiaoning, Kang, Jian, Takano, Takahiro +1 more·2006·Nature Neuroscience

Abstract

Although astrocytes are the most abundant cell type in the brain, evidence for their activation during physiological sensory activity is lacking. Here we show that whisker stimulation evokes increases in astrocytic cytosolic calcium (Ca(2+)) within the barrel cortex of adult mice. Increases in astrocytic Ca(2+) were a function of the frequency of stimulation, occurred within several seconds and were inhibited by metabotropic glutamate receptor antagonists. To distinguish between synaptic input and output, local synaptic activity in cortical layer 2 was silenced by iontophoresis of AMPA and NMDA receptor antagonists. The antagonists did not reduce astrocytic Ca(2+) responses despite a marked reduction in excitatory postsynaptic currents in response to whisker stimulation. These findings indicate that astrocytes respond to synaptic input, by means of spillover or ectopic release of glutamate, and that increases in astrocytic Ca(2+) occur independently of postsynaptic excitatory activity.

Integrity signals

Registry

Crossref · 10.1038/nn1703

Cites

50 of 50 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Astrocytes can detect synaptic activity via glutamatergic receptors.

    “Astrocytes are capable of detecting synaptic activity through glutamatergic receptors [80].”

    From ApoE, ApoE Receptors, and the Synapse in Alzheimer's Disease (2017) · cites this paper as [80]

  • Astrocytes are sensitive to changes in neural activity and respond with increases in intracellular calcium levels.

    “In vivo imaging studies of astrocytes have shown that astrocytes are sensitive to changes in neural activity and respond with increases in intracellular calcium levels (Wang et al., 2006; Winship et al., 2007).”

    From Adult neurogenesis, glia, and the extracellular matrix (2019) · cites this paper as Wang et al., 2006

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wang, X., Lou, N., Xu, Q., Tian, G.-F., Peng, W. G., Han, X., Kang, J., Takano, T., & Nedergaard, M.
Affiliations
  • University of Rochester Medicine (US)
  • New York Medical College (US)
Corresponding author
Nedergaard, Maiken
Venue
Nature Neuroscience
Published
2006-05-14
Volume
9
Issue
6
Pages
816-823
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W2026154459
Cited by
685
References
50
Citations by year
2026: 13 · 2025: 30 · 2024: 15 · 2023: 25 · 2022: 38 · 2021: 24 · 2020: 27 · 2019: 24 · 2018: 31 · 2017: 30 · 2016: 26 · 2015: 37 · 2014: 47 · 2013: 51 · 2012: 52
FWCI
16.47
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Neurological Disorders and Stroke (NS38073, R01NS038073, NS 30007)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
astrocytes, calcium signaling, sensory stimulation, whisker stimulation, barrel cortex, metabotropic glutamate receptors, glutamate spillover, synaptic input, AMPA receptor antagonists, NMDA receptor antagonists, excitatory postsynaptic currents, astrocyte activation
MeSH terms
Afferent Pathways, Animals, Astrocytes, Calcium, Cell Communication, Female, Male, Mechanoreceptors, Synaptic Transmission, Physical Stimulation, Reaction Time, Somatosensory Cortex, Touch, Vibrissae, Up-Regulation, Receptors, N-Methyl-D-Aspartate, Receptors, AMPA, Receptors, Metabotropic Glutamate, Excitatory Amino Acid Antagonists, Excitatory Postsynaptic Potentials, Calcium Signaling, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review