Construction of implantable optical fibers for long-term optogenetic manipulation of neural circuits
Open accessAbstract
In vivo optogenetic strategies have redefined our ability to assay how neural circuits govern behavior. Although acutely implanted optical fibers have previously been used in such studies, long-term control over neuronal activity has been largely unachievable. Here we describe a method to construct implantable optical fibers to readily manipulate neural circuit elements with minimal tissue damage or change in light output over time (weeks to months). Implanted optical fibers readily interface with in vivo electrophysiological arrays or electrochemical detection electrodes. The procedure described here, from implant construction to the start of behavioral experimentation, can be completed in approximately 2-6 weeks. Successful use of implantable optical fibers will allow for long-term control of mammalian neural circuits in vivo, which is integral to the study of the neurobiology of behavior.
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Through tissue-specific expression and local stimulation of light-activated proteins, distinct neural circuits can be rapidly activated or inhibited without affecting neighboring cells.
“Through tissue-specific expression and local stimulation of light-activated proteins, distinct neural circuits can therefore be rapidly activated or inhibited without affecting neighboring cells 102,103.”
From DISSECTING OCD CIRCUITS: FROM ANIMAL MODELS TO TARGETED TREATMENTS: Review: Dissecting OCD Circuits: Animals to Treatments (2015) · cites this paper as [103]
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- Authors
- Sparta, D. R., Stamatakis, A. M., Phillips, J. L., Hovelsø, N., van Zessen, R., & Stuber, G. D.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Stuber, Garret D
- Venue
- Nature Protocols
- Published
- 2011-12-08
- Volume
- 7
- Issue
- 1
- Pages
- 12-23
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- PMC
- PMC8647635
- OpenAlex
- W2082523159
- Cited by
- 394
- References
- 22
- Citations by year
- 2026: 10 · 2025: 15 · 2024: 22 · 2023: 15 · 2022: 22 · 2021: 36 · 2020: 30 · 2019: 48 · 2018: 33 · 2017: 25 · 2016: 33 · 2015: 35 · 2014: 31 · 2013: 23 · 2012: 15
- FWCI
- 9.20
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Alcohol Abuse and Alcoholism (F32AA018610)
- National Institute on Drug Abuse (DA029325, R21DA029325)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- optogenetics, neural circuits, tissue damage, neurobiology of behavior
- MeSH terms
- Electrophysiology, Fiber Optic Technology, Nerve Net, Implants, Experimental, Optical Fibers
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