In vitro functional models for human liver diseases and drug screening: beyond animal testing

Matrix elasticity directs stem cell lineage specification

Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915

Re-epithelialization and immune cell behaviour in an ex vivo human skin model

Rakita, A., Nikolić, N., Mildner, M., Matiasek, J., & Elbe‐Bürger, A. (2020) · Scientific Reports · doi:10.1038/s41598-019-56847-4 · Cited by 8,234

3D bioprinting of tissues and organs

Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034

Cerebral organoids model human brain development and microcephaly

Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372

Reactive Oxygen Species in Inflammation and Tissue Injury

Mittal, M., Siddiqui, M. R., Tran, K., Reddy, S. P., & Malik, A. B. (2014) · Antioxidants & Redox Signaling · doi:10.1089/ars.2012.5149 · Cited by 4,897

Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology

Peppas, N. A., Hilt, J. Z., Khademhosseini, A., & Langer, R. (2006) · Advanced Materials · doi:10.1002/adma.200501612 · Cited by 4,109

Porous scaffold design for tissue engineering

Hollister, S. J. (2005) · Nature Materials · doi:10.1038/nmat1421 · Cited by 3,979

Biomaterials & scaffolds for tissue engineering

O'Brien, F. J. (2011) · Materials Today · doi:10.1016/s1369-7021(11)70058-x · Cited by 3,437

Applications of machine learning in drug discovery and development

Vamathevan, J., Clark, D., Czodrowski, P., Dunham, I., Ferran, E., Lee, G., Li, B., Madabhushi, A., Shah, P., Spitzer, M., et al. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0024-5 · Cited by 3,228

Organogenesis in a dish: Modeling development and disease using organoid technologies

Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931

Vascularized and functional human liver from an iPSC-derived organ bud transplant

Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170

A living biobank of breast cancer organoids captures disease heterogeneity

Sachs, N., de Ligt, J., Kopper, O., Gogola, E., Bounova, G., Weeber, F., Balgobind, A. V., Wind, K., Gracanin, A., Begthel, H., et al. (2018) · Cell · doi:10.1016/j.cell.2017.11.010 · Cited by 1,973

Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues

Miller, J. S., Stevens, K. R., Yang, M. T., Baker, B. M., Nguyen, D.-H. T., Cohen, D. M., Toro, E., Chen, A. A., Galie, P. A., Yu, X., et al. (2012) · Nature Materials · doi:10.1038/nmat3357 · Cited by 1,917

25th Anniversary Article: Engineering Hydrogels for Biofabrication

Malda, J., Visser, J., Melchels, F. P., Jüngst, T., Hennink, W. E., Dhert, W. J. A., Groll, J., & Hutmacher, D. W. (2013) · Advanced Materials · doi:10.1002/adma.201302042 · Cited by 1,901

Is it time to start transitioning from 2D to 3D cell culture?

Jensen, C., & Teng, Y. (2020) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2020.00033 · Cited by 1,640

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

Broutier, L., Mastrogiovanni, G., Verstegen, M. M., Francies, H. E., Gavarró, L. M., Bradshaw, C. R., Allen, G. E., Arnes-Benito, R., Sidorova, O., Gaspersz, M. P., et al. (2017) · Nature Medicine · doi:10.1038/nm.4438 · Cited by 1,471

Printability and shape fidelity of bioinks in 3D bioprinting

Schwab, A., Levato, R., D’Este, M., Piluso, S., Eglin, D., & Malda, J. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00084 · Cited by 1,359

Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering

Annabi, N., Nichol, J. W., Zhong, X., Ji, C., Koshy, S. T., Khademhosseini, A., & Dehghani, F. (2010) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2009.0639 · Cited by 1,352

Engineering organoids

Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity

Jacob, F., Salinas, R. D., Zhang, D. Y., Nguyen, P. T. T., Schnoll, J. G., Wong, S. Z. H., Thokala, R., Sheikh, S., Saxena, D., Prokop, S., et al. (2020) · Cell · doi:10.1016/j.cell.2019.11.036 · Cited by 1,149

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

Matano, M., Date, S., Shimokawa, M., Takano, A., Fujii, M., Ohta, Y., Watanabe, T., Kanai, T., & Sato, T. (2015) · Nature Medicine · doi:10.1038/nm.3802 · Cited by 1,135

Idiosyncratic drug hepatotoxicity

Kaplowitz, N. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1750 · Cited by 1,054

Synthetic alternatives to Matrigel

Aisenbrey, E. A., & Murphy, W. L. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-0199-8 · Cited by 1,016

Liver regeneration: biological and pathological mechanisms and implications

Michalopoulos, G. K., & Bhushan, B. (2021) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-020-0342-4 · Cited by 998

Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting

Ma, X., Qu, X., Zhu, W. Z., Li, Y.-S. J., Yuan, S., Zhang, H., Liu, J., Wang, P., Lai, C. S. E., Zanella, F., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524510113 · Cited by 881

Synthetic polymer scaffolds for tissue engineering

Place, E., George, J. H., Williams, C. K., & Stevens, M. M. (2009) · Chemical Society Reviews · doi:10.1039/b811392k · Cited by 844

Electrospinning for tissue engineering applications

Rahmati, M., Mills, D. K., Urbanska, A. M., Saeb, M. R., Venugopal, J. R., Ramakrishna, S., & Mozafari, M. (2020) · Progress in Materials Science · doi:10.1016/j.pmatsci.2020.100721 · Cited by 842

Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins

Gerets, H. H. J., Tilmant, K., Gerin, B., Chanteux, H., Depelchin, B. O., Dhalluin, S., & Atienzar, F. A. (2012) · Cell Biology and Toxicology · doi:10.1007/s10565-011-9208-4 · Cited by 698

Organoids: A historical perspective of thinking in three dimensions

Simian, M., & Bissell, M. J. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201610056 · Cited by 620

A liver-on-a-chip platform with bioprinted hepatic spheroids

Bhise, N. S., Manoharan, V., Massa, S., Tamayol, A., Ghaderi, M., Miscuglio, M., Lang, Q., Shrike Zhang, Y., Shin, S. R., Calzone, G., et al. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/014101 · Cited by 615

Global Epidemiology of Chronic Liver Disease

Cheemerla, S., & Balakrishnan, M. (2021) · Clinical Liver Disease · doi:10.1002/cld.1061 · Cited by 571

Three-dimensional plotted scaffolds with controlled pore size gradients: Effect of scaffold geometry on mechanical performance and cell seeding efficiency

Sobral, J. M., Caridade, S. G., Sousa, R. A., Mano, J. F., & Reis, R. L. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.11.003 · Cited by 561

A review of key challenges of electrospun scaffolds for tissue-engineering applications

Khorshidi, S., Solouk, A., Mirzadeh, H., Mazinani, S., Lagaron, J. M., Sharifi, S., & Ramakrishna, S. (2015) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1978 · Cited by 545

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.05.007 · Cited by 537

Polycaprolactone-based biomaterials for tissue engineering and drug delivery: Current scenario and challenges

Mondal, D., Griffith, M., & Venkatraman, S. S. (2016) · International Journal of Polymeric Materials · doi:10.1080/00914037.2015.1103241 · Cited by 525

Reproducing human and cross-species drug toxicities using a liver-chip

Jang, K.-J., Otieno, M. A., Ronxhi, J., Lim, H.-K., Ewart, L., Kodella, K. R., Petropolis, D. B., Kulkarni, G., Rubins, J. E., Conegliano, D., et al. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aax5516 · Cited by 511

An artificial liver sinusoid with a microfluidic endothelial‐like barrier for primary hepatocyte culture

Lee, P. J., Hung, P. J., & Lee, L. P. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21360 · Cited by 507

Drug development in the era of precision medicine

Dugger, S. A., Platt, A., & Goldstein, D. B. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.226 · Cited by 493

Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D

Faulkner‐Jones, A., Fyfe, C., Cornelissen, D.-J., Gardner, J., King, J., Courtney, A., & Shu, W. (2015) · Biofabrication · doi:10.1088/1758-5090/7/4/044102 · Cited by 482

Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering

Lu, T., Li, Y., & Chen, T. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s38635 · Cited by 478

Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies

Nuciforo, S., Fofana, I., Matter, M. S., Blumer, T., Calabrese, D., Boldanova, T., Piscuoglio, S., Wieland, S., Ringnalda, F., Schwank, G., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.07.001 · Cited by 473

Open TG-GATEs: a large-scale toxicogenomics database

Igarashi, Y., Nakatsu, N., Yamashita, T., Ono, A., Ohno, Y., Urushidani, T., & Yamada, H. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku955 · Cited by 465

3D Bioprinting: from Benches to Translational Applications

Heinrich, M. A., Liu, W. H., Jimenez, A., Yang, J., Akpek, A., Liu, X., Pi, Q., Mu, X., Hu, N., Schiffelers, R. M., et al. (2019) · Small · doi:10.1002/smll.201805510 · Cited by 455

Commercialization of microfluidic devices

Volpatti, L. R., & Yetisen, A. K. (2014) · Trends in biotechnology · doi:10.1016/j.tibtech.2014.04.010 · Cited by 437

A microfluidic 3D hepatocyte chip for drug toxicity testing

Toh, Y., Lim, T. C., Tai, D., Xiao, G., van Noort, D., & Yu, H. (2009) · Lab on a Chip · doi:10.1039/b900912d · Cited by 430

Is it possible to overcome issues of external validity in preclinical animal research? Why most animal models are bound to fail

Pound, P., & Ritskes-Hoitinga, M. (2018) · Journal of Translational Medicine · doi:10.1186/s12967-018-1678-1 · Cited by 419

3D bioprinting of functional tissue models for personalized drug screening and in vitro disease modeling

Ma, X., Liu, J., Zhu, W. Z., Tang, M., Lawrence, N., Yu, C., Gou, M., & Chen, S. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.06.011 · Cited by 417

Organoids by design

Takebe, T., & Wells, J. M. (2019) · Science · doi:10.1126/science.aaw7567 · Cited by 417

Towards organoid culture without Matrigel

Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409

Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells

Takebe, T., Sekine, K., Kimura, M., Yoshizawa, E., Ayano, S., Koido, M., Funayama, S., Nakanishi, N., Hisai, T., Kobayashi, T., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.005 · Cited by 405

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation

Sampaziotis, F., de Brito, M. C., Madrigal, P., Bertero, A., Saeb‐Parsy, K., Soares, F. A. C., Schrumpf, E., Melum, E., Karlsen, T. H., Bradley, J. A., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3275 · Cited by 398

Principals of neovascularization for tissue engineering

Nomi, M., Atala, A., Coppi, P. D., & Söker, S. (2002) · Molecular Aspects of Medicine · doi:10.1016/s0098-2997(02)00008-0 · Cited by 392

Emerging Biofabrication Strategies for Engineering Complex Tissue Constructs

Pedde, R. D., Mirani, B., Navaei, A., Styan, T., Wong, S., Mehrali, M., Thakur, A., Mohtaram, N. K., Bayati, A., Dolatshahi‐Pirouz, A., et al. (2017) · Advanced Materials · doi:10.1002/adma.201606061 · Cited by 392

Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro

Nguyen, D. G., Funk, J., Robbins, J. B., Crogan‐Grundy, C., Presnell, S. C., Singer, T., & Roth, A. B. (2016) · PLoS ONE · doi:10.1371/journal.pone.0158674 · Cited by 389

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

Mun, S. J., Ryu, J., Lee, M., Son, Y. S., Oh, S. J., Cho, H., Son, M., Kim, S. J., Kim, S. J., Yoo, H. J., et al. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2019.06.030 · Cited by 380

High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell–Derived Organoids

Shinozawa, T., Kimura, M., Cai, Y., Saiki, N., Yoneyama, Y., Ouchi, R., Koike, H., Maezawa, M., Zhang, R.-R., Dunn, A., et al. (2021) · Gastroenterology · doi:10.1053/j.gastro.2020.10.002 · Cited by 378

Hydrogel microenvironments for cancer spheroid growth and drug screening

Li, Y., & Kumacheva, E. (2018) · Science Advances · doi:10.1126/sciadv.aas8998 · Cited by 356

Liver organoids: from basic research to therapeutic applications

Prior, N., Inacio, P., & Huch, M. (2019) · Gut · doi:10.1136/gutjnl-2019-319256 · Cited by 356

Generation of Three-Dimensional Hepatocyte/Gelatin Structures with Rapid Prototyping System

Wang, X., Yan, Y., Pan, Y., Xiong, Z., Liu, H., Cheng, J., Liu, F., Lin, F., Wu, R., Zhang, R., et al. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.83 · Cited by 353

Development of Liver Decellularized Extracellular Matrix Bioink for Three-Dimensional Cell Printing-Based Liver Tissue Engineering

Lee, H., Han, W., Kim, H., Ha, D., Jang, J., Kim, B. S., & Cho, D. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.6b01908 · Cited by 334

Microfluidic-enhanced 3D bioprinting of aligned myoblast-laden hydrogels leads to functionally organized myofibers in vitro and in vivo

Costantini, M., Testa, S., Mozetic, P., Barbetta, A., Fuoco, C., Fornetti, E., Tamiro, F., Bernardini, S., Jaroszewicz, J., Święszkowski, W., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.03.026 · Cited by 334

Structural and functional hepatocyte polarity and liver disease

Gissen, P., & Arias, I. M. (2015) · Journal of Hepatology · doi:10.1016/j.jhep.2015.06.015 · Cited by 333

Bio-inspired detoxification using 3D-printed hydrogel nanocomposites

Gou, M., Qu, X., Zhu, W. Z., Xiang, M., Yang, J., Zhang, K., Wei, Y., & Chen, S. (2014) · Nature Communications · doi:10.1038/ncomms4774 · Cited by 331

Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury

Proctor, W. R., Foster, A. J., Vogt, J., Summers, C., Middleton, B., Pilling, M. A., Shienson, D., Kijanska, M., Ströbel, S., Kelm, J. M., et al. (2017) · Archives of Toxicology · doi:10.1007/s00204-017-2002-1 · Cited by 328

Directed differentiation of cholangiocytes from human pluripotent stem cells

Ogawa, M., Ogawa, S., Bear, C. E., Ahmadi, S., Chin, S., Li, B., Grompe, M., Keller, G. M., Kamath, B. M., & Ghanekar, A. (2015) · Nature Biotechnology · doi:10.1038/nbt.3294 · Cited by 318

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories

Bernal, P. N., Bouwmeester, M., Madrid-Wolff, J. A., Falandt, M., Florczak, S., Rodriguez, N. G., Li, Y., Größbacher, G., Samsom, R., van Wolferen, M. E., et al. (2022) · Advanced Materials · doi:10.1002/adma.202110054 · Cited by 297

Growth of Epithelial Organoids in a Defined Hydrogel

Broguiere, N., Isenmann, L., Hirt, C., Ringel, T., Placzek, S., Cavalli, E., Ringnalda, F., Villiger, L., Züllig, R., Lehmann, R., et al. (2018) · Advanced Materials · doi:10.1002/adma.201801621 · Cited by 282

Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture

Ma, X., Yu, C., Wang, P., Xu, W., Wan, X., Lai, C. S. E., Liu, J., Koroleva-Maharajh, A., & Chen, S. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.09.026 · Cited by 281

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease

Ramli, M. N. B., Lim, Y. S., Koe, C. T., Demircioglu, D., Tng, W., Gonzales, K. A. U., Tan, C. P., Szczerbinska, I., Liang, H., Soe, E. L., et al. (2020) · Gastroenterology · doi:10.1053/j.gastro.2020.06.010 · Cited by 278

A microfluidically perfused three dimensional human liver model

Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., et al. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.043 · Cited by 265

Development of a 3D cell printed construct considering angiogenesis for liver tissue engineering

Lee, J. W., Choi, Y., Yong, W.-J., Pati, F., Shim, J., Kang, K. S., Kang, I.-H., Park, J., & Cho, D. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/015007 · Cited by 257

The Current Status of Drug Discovery and Development as Originated in United States Academia: The Influence of Industrial and Academic Collaboration on Drug Discovery and Development

Takebe, T., Imai, R., & Ono, S. (2018) · Clinical and Translational Science · doi:10.1111/cts.12577 · Cited by 257

Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture by cell--cell contact and cell membranes.

Nakamura, T., Yoshimoto, K., Nakayama, Y., Tomita, Y., & Ichihara, A. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.23.7229 · Cited by 256

Animal models for liver disease – A practical approach for translational research

Nevzorova, Y. A., Boyer‐Díaz, Z., Cubero, F. J., & Gracia‐Sancho, J. (2020) · Journal of Hepatology · doi:10.1016/j.jhep.2020.04.011 · Cited by 252

Hepatocyte polarity

Treyer, A., & Müsch, A. (2013) · Comprehensive Physiology · doi:10.1002/cphy.c120009 · Cited by 252

Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions

Yamada, M., Utoh, R., Ohashi, K., Tatsumi, K., Yamato, M., Okano, T., & Seki, M. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.07.068 · Cited by 247

Human hepatic organoids for the analysis of human genetic diseases

Guan, Y., Xu, D., Garfin, P. M., Ehmer, U., Hurwitz, M., Enns, G., Michie, S., Wu, M., Zheng, M., Nishimura, T., et al. (2017) · JCI Insight · doi:10.1172/jci.insight.94954 · Cited by 243

Engineered Liver-On-A-Chip Platform to Mimic Liver Functions and Its Biomedical Applications: A Review

Deng, J., Wei, W., Chen, Z., Lin, B., Zhao, W., Luo, Y., & Zhang, X. (2019) · Micromachines · doi:10.3390/mi10100676 · Cited by 238

Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro

Leite, S. B., Roosens, T., El Taghdouini, A., Mannaerts, I., Smout, A. J., Najimi, M., Sokal, E., Noor, F., Chesne, C., & van Grunsven, L. A. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2015.11.026 · Cited by 234

A human liver microphysiology platform for investigating physiology, drug safety, and disease models

Vernetti, L. A., Senutovitch, N., Boltz, R. C., DeBiasio, R., Shun, T. Y., Gough, A., & Taylor, D. L. (2015) · Experimental Biology and Medicine · doi:10.1177/1535370215592121 · Cited by 233

Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis

Coll, M., Perea, L., Boon, R., Leite, S. B., Vallverdú, J., Mannaerts, I., Smout, A., El Taghdouini, A., Blaya, D., Rodrigo-Torres, D., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.05.027 · Cited by 233

Liver-cell patterning lab chip: mimicking the morphology of liver lobule tissue

Ho, C.-T., Lin, R.-Z., Chen, R.-J., Chin, C.-K., Gong, S.-E., Chang, H.-Y., Peng, H.-L., Hsu, L., Yew, T.-R., Chang, S.-F., et al. (2013) · Lab on a Chip · doi:10.1039/c3lc50402f · Cited by 230

Cell sources for in vitro human liver cell culture models

Zeilinger, K., Freyer, N., Damm, G., Seehofer, D., & Knöspel, F. (2016) · Experimental Biology and Medicine · doi:10.1177/1535370216657448 · Cited by 230

Fabrication of liver microtissue with liver decellularized extracellular matrix (dECM) bioink by digital light processing (DLP) bioprinting

Mao, Q., Wang, Y., Li, Y., Juengpanich, S., Li, W., Chen, M., Yin, J., Fu, J., & Cai, X. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.110625 · Cited by 222

Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies

Castell, J. V., Jover, R., Martinez‐Jimenez, C. P., & G�mez-Lech�n, M. J. (2006) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2.2.183 · Cited by 219

Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids

Artegiani, B., van Voorthuijsen, L., Lindeboom, R. G. H., Seinstra, D., Heo, I., Tapia, P., López-Iglesias, C., Postrach, D., Dayton, T., Oka, R., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.04.017 · Cited by 218

Liver ‘organ on a chip’

Beckwitt, C. H., Clark, A. M., Wheeler, S., Taylor, D. L., Stolz, D. B., Griffith, L. G., & Wells, A. H. (2017) · Experimental Cell Research · doi:10.1016/j.yexcr.2017.12.023 · Cited by 216

Human primary liver cancer organoids reveal intratumor and interpatient drug response heterogeneity

Li, L., Knútsdóttir, H., Hui, K., Weiss, M. J., He, J. P., Philosophe, B., Cameron, A. M., Wolfgang, C. L., Pawlik, T. M., Ghiaur, G., et al. (2019) · JCI Insight · doi:10.1172/jci.insight.121490 · Cited by 216

Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury

Wang, S., Wang, X., Tan, Z., Su, Y., Liu, J., Chang, M., Yan, F., Chen, J., Chen, T., Li, C., et al. (2019) · Cell Research · doi:10.1038/s41422-019-0242-8 · Cited by 214

Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells

Dianat, N., Dubois‐Pot‐Schneider, H., Steichen, C., Desterke, C., Leclerc, P., Raveux, A., Combettes, L., Weber, A., Corlu, A., & Dubart‐Kupperschmitt, A. (2014) · Hepatology · doi:10.1002/hep.27165 · Cited by 213

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Wu, Y., Lin, Z. Y., Wenger, A., Tam, K. C., & Tang, X. S. (2017) · Bioprinting · doi:10.1016/j.bprint.2017.12.001 · Cited by 212

Seminars in Liver Disease

Huebert, A. A. A. N. J.-S. R. C. (2026) · Seminars in Liver Disease · doi:10.1055/s-00000069 · Cited by 210

Fabrication of scalable and structured tissue engineering scaffolds using water dissolvable sacrificial 3D printed moulds

Mohanty, S., Larsen, L. B., Trifol, J., Szabó, P., Burri, H., Canali, C., Dufva, M., Emnéus, J., & Wolff, A. (2015) · Materials Science and Engineering C · doi:10.1016/j.msec.2015.06.002 · Cited by 202

Mediators of Inflammation

Galvão, I., Sugimoto, M. A., Vago, J. P., Machado, M. G., & Sousa, L. P. (2018) · Springer eBooks · doi:10.1007/978-3-319-77658-3_1 · Cited by 202

Animal models of drug-induced liver injury

McGill, M. R., & Jaeschke, H. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2018.08.037 · Cited by 202

Cell-printed 3D liver-on-a-chip possessing a liver microenvironment and biliary system

Lee, H., Chae, S., Kim, J. Y., Han, W., Kim, J., Choi, Y., & Cho, D. (2018) · Biofabrication · doi:10.1088/1758-5090/aaf9fa · Cited by 199

A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate®

Bircsak, K. M., DeBiasio, R., Miedel, M. T., Alsebahi, A., Reddinger, R. M., Saleh, A. D., Shun, T., Vernetti, L. A., & Gough, A. (2021) · Toxicology · doi:10.1016/j.tox.2020.152667 · Cited by 198

Bioprinting towards physiologically relevant tissue models for pharmaceutics

Peng, W., Unutmaz, D., & Ozbolat, I. T. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2016.05.013 · Cited by 196

Recapitulation of hepatitis B virus–host interactions in liver organoids from human induced pluripotent stem cells

Nie, Y., Zheng, Y., Miyakawa, K., Murata, S., Zhang, R., Sekine, K., Ueno, Y., Takebe, T., Wakita, T., Ryo, A., et al. (2018) · EBioMedicine · doi:10.1016/j.ebiom.2018.08.014 · Cited by 188

Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology

Li, S., Xiong, Z., Wang, X., Yan, Y., Liu, H., & Zhang, R. (2009) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911509104094 · Cited by 181

Liver Zonation – Revisiting Old Questions With New Technologies

Cunningham, R. P., & Porat‐Shliom, N. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.732929 · Cited by 181

Microengineered Multi‐Organoid System from hiPSCs to Recapitulate Human Liver‐Islet Axis in Normal and Type 2 Diabetes

Tao, T., Deng, P., Wang, Y., Zhang, X., Guo, Y., Chen, W., & Qin, J. (2022) · Advanced Science · doi:10.1002/advs.202103495 · Cited by 178

3D-printed gelatin scaffolds of differing pore geometry modulate hepatocyte function and gene expression

Lewis, P. L., Green, R. M., & Shah, R. N. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.12.042 · Cited by 172

A Chemically Defined Hydrogel for Human Liver Organoid Culture

Ye, S., Boeter, J. W. B., Mihajlovic, M., van Steenbeek, F. G., van Wolferen, M. E., Oosterhoff, L. A., Marsee, A., Caiazzo, M., van der Laan, L. J. W., Penning, L. C., et al. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202000893 · Cited by 170

Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies

Underhill, G. H., & Khetani, S. R. (2017) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2017.11.012 · Cited by 169

In vitro analysis of a hepatic device with intrinsic microvascular-based channels

Carraro, A., Hsu, W.-M., Kulig, K. M., Cheung, W. S., Miller, M. L., Weinberg, E. J., Swart, E. F., Kaazempur-Mofrad, M., Borenstein, J. T., Vacanti, J. P., et al. (2008) · Biomedical Microdevices · doi:10.1007/s10544-008-9194-3 · Cited by 168

Cell Plasticity in Liver Regeneration

Li, W., Li, L., & Hui, L. (2020) · Trends in Cell Biology · doi:10.1016/j.tcb.2020.01.007 · Cited by 167

Vascularized Liver Organoids Generated Using Induced Hepatic Tissue and Dynamic Liver‐Specific Microenvironment as a Drug Testing Platform

Jin, Y., Kim, J., Lee, J. S., Min, S., Kim, S., Ahn, D., Kim, Y., & Cho, S. (2018) · Advanced Functional Materials · doi:10.1002/adfm.201801954 · Cited by 166

Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric

Yokoyama, Y., Hattori, S., Yoshikawa, C., Yasuda, Y., Koyama, H., Takato, T., & Kobayashi, H. (2009) · Materials Letters · doi:10.1016/j.matlet.2008.12.042 · Cited by 165

3D biofabrication of vascular networks for tissue regeneration: A report on recent advances

Sarker, M. D., Naghieh, S., Sharma, N. K., & Chen, D. (2018) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2018.08.005 · Cited by 164

Organoids to model liver disease

Nuciforo, S., & Heim, M. H. (2021) · JHEP Reports · doi:10.1016/j.jhepr.2020.100198 · Cited by 164

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Volpi, M., Paradiso, A., Costantini, M., & Święszkowski, W. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c01145 · Cited by 164

Hepatic organoids for microfluidic drug screening

Au, S. H., Chamberlain, M. D., Mahesh, S., Sefton, M. V., & Wheeler, A. R. (2014) · Lab on a Chip · doi:10.1039/c4lc00531g · Cited by 162

3D in vitro models of liver fibrosis

van Grunsven, L. A. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.07.004 · Cited by 162

Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling

Akbari, S., Sevinç, G. G., Ersoy, N., Başak, O., Kaplan, K. N., Sevinç, K., Ozel, E., Şengün, B., Enüstün, E., Özçimen, B., et al. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.08.007 · Cited by 158

Direct Construction of a Three-dimensional Structure with Cells and Hydrogel

Yan, Y., Wang, X., Xiong, Z., Liu, H., Liu, F., Lin, F., Wu, R., Zhang, R., & Lu, Q. (2005) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911505053658 · Cited by 155

Bioprinted 3D Primary Human Intestinal Tissues Model Aspects of Native Physiology and ADME/Tox Functions

Madden, L. R., Nguyen, T. V., Garcia-Mojica, S., Shah, V., Le, A. V., Peier, A. M., Visconti, R. J., Parker, E. M., Presnell, S. C., Nguyen, D. G., et al. (2018) · iScience · doi:10.1016/j.isci.2018.03.015 · Cited by 154

3D Primary Hepatocyte Culture Systems for Analyses of Liver Diseases, Drug Metabolism, and Toxicity: Emerging Culture Paradigms and Applications

Lauschke, V. M., Shafagh, R. Z., Hendriks, D. F. G., & Ingelman‐Sundberg, M. (2019) · Biotechnology Journal · doi:10.1002/biot.201800347 · Cited by 153

Online oxygen monitoring using integrated inkjet-printed sensors in a liver-on-a-chip system

Moya, A., Ortega‐Ribera, M., Villalba, X. G., Sowade, E., Zea, M., Illa, X., Ramón, E., Villa, R., Gracia‐Sancho, J., & Gabriel, G. (2018) · Lab on a Chip · doi:10.1039/c8lc00456k · Cited by 150

A novel system for evaluation of drug mixtures for potential efficacy in treating multidrug resistant cancers

Tatosian, D. A., & Shuler, M. L. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22219 · Cited by 149

Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function

Saheli, M., Sepantafar, M., Pournasr, B., Farzaneh, Z., Vosough, M., Piryaei, A., & Baharvand, H. (2018) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26622 · Cited by 148

Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine

Xie, F., Sun, L., Pang, Y., Xu, G., Jin, B., Xu, H., Lu, X., Xu, Y., Du, S., Wang, Y., et al. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120416 · Cited by 147

Development of a biomimetic liver tumor-on-a-chip model based on decellularized liver matrix for toxicity testing

Lu, S., Cuzzucoli, F., Jiang, J., Liang, L.-G., Wang, Y., Kong, M., Zhao, X., Cui, W., Li, J., & Wang, S. (2018) · Lab on a Chip · doi:10.1039/c8lc00852c · Cited by 144

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

Lee-Montiel, F. T., George, S. M., Gough, A., Sharma, A. D., Wu, J., DeBiasio, R., Vernetti, L. A., & Taylor, D. L. (2017) · Experimental Biology and Medicine · doi:10.1177/1535370217703978 · Cited by 141

Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies

Hiller, T., Berg, J., Elomaa, L., Röhrs, V., Ullah, I., Schaar, K., Dietrich, A.-C., Al‐Zeer, M. A., Kurtz, A., Hocke, A. C., et al. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19103129 · Cited by 141

Human stem cell-derived hepatocytes as a model for hepatitis B virus infection, spreading and virus-host interactions

Xia, Y., Carpentier, A., Cheng, X., Block, P. D., Zhao, Y., Zhang, Z., Protzer, U., & Liang, T. J. (2016) · Journal of Hepatology · doi:10.1016/j.jhep.2016.10.009 · Cited by 139

Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro

Skardal, A., Devarasetty, M., Rodman, C., Atala, A., & Söker, S. (2015) · Annals of Biomedical Engineering · doi:10.1007/s10439-015-1298-3 · Cited by 138

A critical perspective on 3D liver models for drug metabolism and toxicology studies

Serras, A. S., Rodrigues, J. S., Cipriano, M., Rodrigues, A. V., Oliveira, N. G., & Miranda, J. P. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.626805 · Cited by 138

On being the right size: scaling effects in designing a human-on-a-chip

Moraes, C., Labuz, J. M., Leung, B. M., Inoue, M., Chun, T.-H., & Takayama, S. (2013) · Integrative Biology · doi:10.1039/c3ib40040a · Cited by 134

Microfluidic platform for real-time signaling analysis of multiple single T cells in parallel

Faley, S. L., Seale, K. T., Hughey, J., Schaffer, D. K., VanCompernolle, S. E., McKinney, B. A., Baudenbacher, F. J., Unutmaz, D., & Wikswo, J. P. (2008) · Lab on a Chip · doi:10.1039/b719799c · Cited by 134

3D bioprinting of liver spheroids derived from human induced pluripotent stem cells sustain liver function and viability in vitro

Goulart, E., de Caires-Junior, L. C., Telles-Silva, K. A., Araújo, B. H. S., Rocco, S. A., Sforça, M. L., de Sousa, I. L., Kobayashi, G. S., Musso, C. M., Assoni, A. F., et al. (2019) · Biofabrication · doi:10.1088/1758-5090/ab4a30 · Cited by 134

Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold

Ng, S. S., Saeb-Parsy, K., Blackford, S. J. I., Segal, J. M., Serra, M. P., Horcas-Lopez, M., No, D. Y., Mastoridis, S., Jassem, W., Frank, C. W., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.07.043 · Cited by 133

A perfusion incubator liver chip for 3D cell culture with application on chronic hepatotoxicity testing

Yu, F., Deng, R., Tong, W. H., Li, H., Ng, C. W., IslamBadhan, A., Iliescu, C., & Yu, H. (2017) · Scientific Reports · doi:10.1038/s41598-017-13848-5 · Cited by 129

3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model

Cuvellier, M., Ezan, F., Oliveira, H., Rose, S., Fricain, J.-C., Langouët, S., Legagneux, V., & Baffet, G. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120611 · Cited by 126

Nanofibrous PLGA electrospun scaffolds modified with type I collagen influence hepatocyte function and support viability in vitro

Brown, J. H., Das, P., DiVito, M. D., Ivancic, D., Tan, L. P., & Wertheim, J. A. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.02.009 · Cited by 114

Preparation, characterization, and evaluation of genipin crosslinked chitosan/gelatin three-dimensional scaffolds for liver tissue engineering applications

Zhang, Y., Wang, Q., Yan, K., Qi, Y., Wang, G., & Cui, Y. (2016) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35717 · Cited by 113

Drug-Induced Liver Injury: Biomarkers, Requirements, Candidates, and Validation

Meunier, L., & Larrey, D. G. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.01482 · Cited by 112

Prediction of human drug-induced liver injury (DILI) in relation to oral doses and blood concentrations

Albrecht, W., Kappenberg, F., Brecklinghaus, T., Stöber, R. M., Marchan, R., Zhang, M., Ebbert, K. E., Kirschner, H., Grinberg, M., Leist, M., et al. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02492-9 · Cited by 110

Polymer Hydrogels to Guide Organotypic and Organoid Cultures

Magno, V., Meinhardt, A., & Werner, C. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202000097 · Cited by 110

A transcriptomics data-driven gene space accurately predicts liver cytopathology and drug-induced liver injury

Kohonen, P. J., Parkkinen, J., Willighagen, E., Ceder, R., Wennerberg, K., Samuel Kaski, A., & Grafström, R. (2017) · Nature Communications · doi:10.1038/ncomms15932 · Cited by 109

Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research

Sun, L., Yang, H., Wang, Y., Zhang, X., Jin, B., Xie, F., Jin, Y., Pang, Y., Zhao, H., Lu, X., et al. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.00878 · Cited by 109

Organoid biobanking: identifying the ethics

Boers, S. N., van Delden, J. J. M., Clevers, H. C., & Bredenoord, A. L. (2016) · EMBO Reports · doi:10.15252/embr.201642613 · Cited by 106

Extending Hepatocyte Functionality for Drug-Testing Applications Using High-Viscosity Alginate–Encapsulated Three-Dimensional Cultures in Bioreactors

Miranda, J. P., Rodrigues, A., Tostões, R. M., Leite, S. B., Zimmerman, H., Carrondo, M. J. T., & Alves, P. M. (2010) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2009.0784 · Cited by 102

Human Nonalcoholic Steatohepatitis on a Chip

Freag, M. S., Namgung, B., Reyna Fernandez, M. E., Gherardi, E., Sengupta, S., & Jang, H. L. (2021) · Hepatology Communications · doi:10.1002/hep4.1647 · Cited by 102

A comprehensive evaluation of metabolic activity and intrinsic clearance in suspensions and monolayer cultures of cryopreserved primary human hepatocytes

Smith, C. M., Nolan, C. K., Edwards, M., Hatfield, J. B., Stewart, T. W., Ferguson, S. S., LeCluyse, E. L., & Sahi, J. (2012) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.23262 · Cited by 101

On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro

Jang, M., Neužil, P., Volk, T. J., Manz, A., & Kleber, A. (2015) · Biomicrofluidics · doi:10.1063/1.4922863 · Cited by 100

Grafting of 3D Bioprinting to In Vitro Drug Screening: A Review

Nie, J., Gao, Q., Fu, J., & He, Y. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901773 · Cited by 100

DeepDILI: Deep Learning-Powered Drug-Induced Liver Injury Prediction Using Model-Level Representation

Li, T., Tong, W., Roberts, R. A., Liu, Z., & Thakkar, S. (2020) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.0c00374 · Cited by 100

Three-dimensional printing of chemically crosslinked gelatin hydrogels for adipose tissue engineering

Negrini, N. C., Celikkin, N., Tarsini, P., Farè, S., & Święszkowski, W. (2019) · Biofabrication · doi:10.1088/1758-5090/ab56f9 · Cited by 98

Mimicking Native Liver Lobule Microarchitecture In Vitro with Parenchymal and Non-parenchymal Cells Using 3D Bioprinting for Drug Toxicity and Drug Screening Applications

Janani, G., Priya, S., Dey, S., & Mandal, B. B. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c00312 · Cited by 98

3D bioprinting of bicellular liver lobule-mimetic structures via microextrusion of cellulose nanocrystal-incorporated shear-thinning bioink

Wu, Y., Wenger, A., Golzar, H., & Tang, X. S. (2020) · Scientific Reports · doi:10.1038/s41598-020-77146-3 · Cited by 95

A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

Klotz, B. J., Oosterhoff, L. A., Utomo, L., Lim, K. S., Vallmajó-Martín, Q., Clevers, H. C., Woodfield, T. B. F., Rosenberg, A. J. W. P., Malda, J., Ehrbar, M., et al. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201900979 · Cited by 93

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

Krüger, M., Oosterhoff, L. A., van Wolferen, M. E., Schiele, S., Walther, A., Geijsen, N., Laporte, L. D., van der Laan, L. J. W., Kock, L. M., & Spee, B. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901658 · Cited by 93

Blended electrospinning with human liver extracellular matrix for engineering new hepatic microenvironments

Grant, R., Hallett, J. M., Forbes, S. J., Hay, D. C., & Callanan, A. (2019) · Scientific Reports · doi:10.1038/s41598-019-42627-7 · Cited by 91

Modeling alcohol-associated liver disease in a human Liver-Chip

Nawroth, J. C., Petropolis, D. B., Manatakis, D. V., Maulana, T. I., Burchett, G., Schlünder, K., Witt, A., Shukla, A., Kodella, K., Ronxhi, J., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109393 · Cited by 88

In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device

Skardal, A., Devarasetty, M., Söker, S., & Hall, A. R. (2015) · Biofabrication · doi:10.1088/1758-5090/7/3/031001 · Cited by 87

Degradable hydrogels derived from PEG‐diacrylamide for hepatic tissue engineering

Stevens, K. R., Miller, J. S., Blakely, B. L., Chen, C. S., & Bhatia, S. N. (2015) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35478 · Cited by 83

Liver Tissue Engineering: Challenges and Opportunities

Agarwal, T., Subramanian, B., & Maiti, T. K. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00745 · Cited by 83

Bioprinted liver provides early insight into the role of Kupffer cells in TGF-β1 and methotrexate-induced fibrogenesis

Norona, L. M., Nguyen, D. G., Gerber, D. A., Presnell, S. C., Mosedale, M., & Watkins, P. B. (2019) · PLoS ONE · doi:10.1371/journal.pone.0208958 · Cited by 81

Organoid culture media formulated with growth factors of defined cellular activity

Urbischek, M., Rannikmäe, H., Foets, T., Ravn, K., Hyvönen, M., & de la Roche, M. (2019) · Scientific Reports · doi:10.1038/s41598-019-42604-0 · Cited by 81

Consent for governance in the ethical use of organoids

Boers, S. N., & Bredenoord, A. L. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0112-5 · Cited by 80

One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs

Wang, Y., Liu, H., Zhang, M., Wang, H., Chen, W., & Qin, J. (2020) · Biomaterials Science · doi:10.1039/d0bm01085e · Cited by 78

Injectable Liver: A Novel Approach Using Fibrin Gel as a Matrix for Culture and Intrahepatic Transplantation of Hepatocytes

Bruns, H., Kneser, U., Holzhüter, S., Roth, B., Kluth, J., Kaufmann, P. M., Kluth, D., & Fiegel, H. C. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1718 · Cited by 77

Small RNAs in eucaryotes: new clues for amplifying microRNA benefits

Ledda, B., Ottaggio, L., Izzotti, A., Sukkar, S. G., & Miele, M. (2020) · Cell & Bioscience · doi:10.1186/s13578-019-0370-3 · Cited by 76

Recent Applications of Three Dimensional Printing in Cardiovascular Medicine

Gardin, C., Ferroni, L., Latrémouille, C., Chachques, J. C., Mitrečić, D., & Zavan, B. (2020) · Cells · doi:10.3390/cells9030742 · Cited by 76

Biofabrication of Hepatic Constructs by 3D Bioprinting of a Cell‐Laden Thermogel: An Effective Tool to Assess Drug‐Induced Hepatotoxic Response

Gori, M., Giannitelli, S. M., Torre, M., Mozetic, P., Abbruzzese, F., Trombetta, M., Traversa, E., Moroni, L., & Rainer, A. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.202001163 · Cited by 76

Liver organoids reproduce alpha-1 antitrypsin deficiency-related liver disease

Gómez‐Mariano, G., Matamala, N., Martínez, S., Justo, I., Marcacuzco, A., Jiménez, C., Monzón, S., Cuesta, I., Garfia, C., Martínez, M. T., et al. (2019) · Hepatology International · doi:10.1007/s12072-019-10007-y · Cited by 75

Production of Multiple Cell‐Laden Microtissue Spheroids with a Biomimetic Hepatic‐Lobule‐Like Structure

Hong, G., Kim, J., Oh, H., Yun, S., Kim, C. M., Jeong, Y., Yun, W., Shim, J., Jang, I., Kim, C., et al. (2021) · Advanced Materials · doi:10.1002/adma.202102624 · Cited by 71

In Vitro Evaluation of Major In Vivo Drug Metabolic Pathways Using Primary Human Hepatocytes and HepaRG Cells in Suspension and a Dynamic Three-Dimensional Bioreactor System

Darnell, M., Ulvestad, M., Ellis, E. C. S., Weidolf, L. O. G., & Andersson, T. B. (2012) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.112.195834 · Cited by 67

Organoids from adult liver and pancreas: Stem cell biology and biomedical utility

Hindley, C. J., Cordero-Espinoza, L., & Huch, M. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.06.039 · Cited by 67

Long‐term liver‐specific functions of hepatocytes in electrospun chitosan nanofiber scaffolds coated with fibronectin

Rajendran, D., Hussain, A., Yip, D., Parekh, A., Shrirao, A. B., & Cho, C. H. (2017) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36072 · Cited by 66

A Polyurethane-Gelatin Hybrid Construct for Manufacturing Implantable Bioartificial Livers

Xu, W., Wang, X., Yan, Y., & Zhang, R. (2008) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911508095517 · Cited by 65

A Microfabricated Platform for Generating Physiologically-Relevant Hepatocyte Zonation

McCarty, W. J., Usta, O. B., & Yarmush, M. L. (2016) · Scientific Reports · doi:10.1038/srep26868 · Cited by 65

Engineering Liver Microtissues for Disease Modeling and Regenerative Medicine

Huang, D. (., Gibeley, S. B., Xu, C., Xiao, Y., Celik, O., Ginsberg, H. N., & Leong, K. W. (2020) · Advanced Functional Materials · doi:10.1002/adfm.201909553 · Cited by 65

The challenge of developing human 3D organoids into medicines

Vives, J., & Batlle-Morera, L. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-1586-1 · Cited by 65

Tunable Cross-Linking and Adhesion of Gelatin Hydrogels via Bioorthogonal Click Chemistry

Negrini, N. C., Volponi, A. A., Sharpe, P. T., & Celiz, A. D. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00136 · Cited by 64

Nanobead-on-string composites for tendon tissue engineering

Rinoldi, C., Kijeńska‐Gawrońska, E., Chlanda, A., Choińska, E., Khenoussi, N., Tamayol, A., Khademhosseini, A., & Święszkowski, W. (2018) · Journal of Materials Chemistry B · doi:10.1039/c8tb00246k · Cited by 61

In Vitro Human Liver Model of Nonalcoholic Steatohepatitis by Coculturing Hepatocytes, Endothelial Cells, and Kupffer Cells

Suurmond, C. E., Lasli, S., van den Dolder, F. W., Ung, A., Kim, H., Bandaru, P., Lee, K., Cho, H., Ahadian, S., Ashammakhi, N., et al. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201901379 · Cited by 61

Mimicking Tumor Hypoxia in Non-Small Cell Lung Cancer Employing Three-Dimensional In Vitro Models

Ziółkowska‐Suchanek, I. (2021) · Cells · doi:10.3390/cells10010141 · Cited by 61

A Drug-Induced Hybrid Electrospun Poly-Capro-Lactone: Cell-Derived Extracellular Matrix Scaffold for Liver Tissue Engineering

Grant, R., Hay, D. C., & Callanan, A. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0419 · Cited by 60

Characterization of hepatocyte-based in vitro systems for reliable toxicity testing

Vinken, M., & Hengstler, J. G. (2018) · Archives of Toxicology · doi:10.1007/s00204-018-2297-6 · Cited by 57

Multicellular Co-Culture in Three-Dimensional Gelatin Methacryloyl Hydrogels for Liver Tissue Engineering

Cui, J., Wang, H., Shi, Q., Sun, T., Huang, Q., & Fukuda, T. (2019) · Molecules · doi:10.3390/molecules24091762 · Cited by 57

A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features

Sgodda, M., Dai, Z., Zweigerdt, R., Sharma, A. D., Ott, M., & Cantz, T. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0023 · Cited by 54

Alginate-based tissue-specific bioinks for multi-material 3D-bioprinting of pancreatic islets and blood vessels: A step towards vascularized pancreas grafts

Idaszek, J., Volpi, M., Paradiso, A., Quoc, M. N., Górecka, Ż., Klak, M., Tymicki, G., Berman, A., Wierzbicki, M., Jaworski, S., et al. (2021) · Bioprinting · doi:10.1016/j.bprint.2021.e00163 · Cited by 54

Human hepatocytes loaded in 3D bioprinting generate mini-liver

Zhong, C., Xie, H., Zhou, L., Xu, X., & Zheng, S. (2016) · Hepatobiliary & pancreatic diseases international · doi:10.1016/s1499-3872(16)60119-4 · Cited by 53

Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues

Lee, B. H., Shirahama, H., Kim, M. H., Lee, J. H., Cho, N.-J., & Tan, L. P. (2017) · NPG Asia Materials · doi:10.1038/am.2017.126 · Cited by 52

A microfluidic patterned model of non-alcoholic fatty liver disease: applications to disease progression and zonation

Bulutoglu, B., Rey-Bedón, C., Kang, Y. B., Mert, S., Yarmush, M. L., & Usta, O. B. (2019) · Lab on a Chip · doi:10.1039/c9lc00354a · Cited by 52

Biofabricating murine and human myo‐substitutes for rapid volumetric muscle loss restoration

Costantini, M., Testa, S., Fornetti, E., Fuoco, C., Riera, C. S., Nie, M., Bernardini, S., Rainer, A., Baldi, J., Zoccali, C., et al. (2021) · EMBO Molecular Medicine · doi:10.15252/emmm.202012778 · Cited by 52

Investigation and comparison of new galactosylation methods on PCL/chitosan scaffolds for enhanced liver tissue engineering

Ghahremanzadeh, F., Alihosseini, F., & Semnani, D. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.01.158 · Cited by 51

Synthesis and characterization of site selective photo-crosslinkable glycidyl methacrylate functionalized gelatin-based 3D hydrogel scaffold for liver tissue engineering

Sk, M. M., Das, P., Panwar, A., & Tan, L. P. (2021) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.111694 · Cited by 51

Bioprinting of Human Liver‐Derived Epithelial Organoids for Toxicity Studies

Bouwmeester, M., Bernal, P. N., Oosterhoff, L. A., van Wolferen, M. E., Lehmann, V., Vermaas, M., Buchholz, M., Peiffer, Q. C., Malda, J., van der Laan, L. J. W., et al. (2021) · Macromolecular Bioscience · doi:10.1002/mabi.202100327 · Cited by 51

Gene Expression Data Based Deep Learning Model for Accurate Prediction of Drug-Induced Liver Injury in Advance

Feng, C., Chen, H., Yuan, X., Sun, M., Chu, K., Liu, H., & Rui, M. (2019) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.9b00143 · Cited by 46

Techniques for fabrication and construction of three-dimensional bioceramic scaffolds: Effect on pores size, porosity and compressive strength

Darus, F., Isa, R. M., Mamat, N., & Mariatti, M. (2018) · Ceramics International · doi:10.1016/j.ceramint.2018.07.056 · Cited by 45

A novel 3D liver organoid system for elucidation of hepatic glucose metabolism

Lü, Y., Zhang, G., Shen, C., Uygun, K., Yarmush, M. L., & Meng, Q. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23349 · Cited by 44

Recent advances in chemically defined and tunable hydrogel platforms for organoid culture

Agarwal, T., Celikkin, N., Costantini, M., Maiti, T. K., & Makvandi, P. (2021) · Bio-Design and Manufacturing · doi:10.1007/s42242-021-00126-7 · Cited by 44

Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures

Kim, Y., Kang, K., Yoon, S., Kim, J. S., Park, S. A., Kim, W. D., Lee, S. B., Ryu, K., Jeong, J., & Choi, D. (2018) · Organogenesis · doi:10.1080/15476278.2018.1423931 · Cited by 42

In vitro liver model using microfabricated scaffolds in a modular bioreactor

Vinci, B., Cavallone, D., Vozzi, G., Mazzei, D., Domenici, C., Brunetto, M. R., & Ahluwalia, A. D. (2009) · Biotechnology Journal · doi:10.1002/biot.200900074 · Cited by 41

Fibrin Hydrogels for Endothelialized Liver Tissue Engineering with a Predesigned Vascular Network

Wang, X., & Liu, C. (2018) · Polymers · doi:10.3390/polym10101048 · Cited by 41

BMP antagonists in tissue development and disease

Correns, A., Zimmermann, L. A., Baldock, C., & Sengle, G. (2021) · Matrix Biology Plus · doi:10.1016/j.mbplus.2021.100071 · Cited by 41

An in vitro hepatic zonation model with a continuous oxygen gradient in a microdevice

Sato, A., Kadokura, K., Uchida, H., & Tsukada, K. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.10.017 · Cited by 40

From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications

Huang, Y., Liu, T., Huang, Q., & Wang, Y. (2024) · ACS Sensors · doi:10.1021/acssensors.4c00004 · Cited by 97

3D bioprinting of liver models: A systematic scoping review of methods, bioinks, and reporting quality

Ali, A. S. M., Wu, D., Bannach‐Brown, A., Dhamrait, D., Berg, J., Tolksdorf, B., Lichtenstein, D., Dressler, C., Braeuning, A., Kurreck, J., et al. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.100991 · Cited by 45

Designer cellular spheroids with DNA origami for drug screening

Wei, J., Sun, Y., Wang, H., Zhu, T., Li, L., Zhou, Y., Liu, Q., Dai, Z., Li, W., Yang, T., et al. (2024) · Science Advances · doi:10.1126/sciadv.ado9880 · Cited by 40

3D Bioprinting of an Endothelialized Liver Lobule-like Construct as a Tumor-Scale Drug Screening Platform

Fan, Z., Wei, X., Chen, K., Wang, L., & Xu, M. (2023) · Micromachines · doi:10.3390/mi14040878 · Cited by 41

Genome-wide pan-GPCR cell libraries accelerate drug discovery

Yang, H., Wang, Y., Liu, W., He, T., Liao, J., Qian, Z., Zhao, J., Cong, Z., Sun, D., Liu, Z., et al. (2024) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2024.06.023 · Cited by 37

In Vitro Models of the Liver: Disease Modeling, Drug Discovery and Clinical Applications

Collins, S. D., Yuen, G., Tu, T., Budzinska, M. A., Spring, K. J., Bryant, K., & Shackel, N. A. (2019) · Codon Publications eBooks · doi:10.15586/hepatocellularcarcinoma.2019.ch3 · Cited by 42

Advances in liver organoids: model systems for liver disease

Lee, J., Mun, S. J., Shin, Y., Lee, S., & Son, M. J. (2022) · Archives of Pharmacal Research · doi:10.1007/s12272-022-01390-6 · Cited by 21

Advances in Liver Organoid Technology: A Paradigm Shift in Hepatic Research and Therapeutics

Pentela, B., Mazumder, R., & Mazumder, A. (2026) · Current Pharmaceutical Design · doi:10.2174/0113816128440756260210044332

Emerging trends in modeling human liver disease in vitro

Underhill, G. H., & Khetani, S. R. (2019) · APL Bioengineering · doi:10.1063/1.5119090 · Cited by 26

Liver organoid platforms for disease modeling and drug testing

Cho, S. (2022) · Annals of Hepato-Biliary-Pancreatic Surgery · doi:10.14701/ahbps.2022s1.br-1-4