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Cited by 164Sleep and Wakefulness ResearchSleep and Work-Related FatigueCircadian rhythm and melatonin

Number and Function of Circulating Human Antigen Presenting Cells Regulated by Sleep

Dimitrov, Stoyan, Lange, Tanja, Nohroudi, Klaus, Born, Jan·2007·Sleep
DOI 10.1093/sleep/30.4.401
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Abstract

STUDY OBJECTIVES: There is evidence that sleep facilitates the adaptive immune response to infectious agents and, thereby, supports immunologic memory. The effect might be attained by sleep-induced changes in the number and function of dendritic cells (DCs), which play a key role in the initiation of the immune response. This study aimed to dissociate effects of sleep and circadian rhythm on circulating numbers of DC precursors, ie, CD14+CD16- and CD14(dim)CD16+ monocytes, myeloid dendritic cell precursors (pre-mDC), and plasmacytoid dendritic cells (PDC) and on 2 key cytokines produced by these cells, ie, interleukin (IL)-12 and interferon (IFN)-alpha. DESIGN: In a within-subject cross-over design, human subjects were examined on 2 occasions, ie, during a normal sleep-wake cycle and during 24 hours of wakefulness. Blood was sampled every 1.5 hours during nighttime and every 3 hours during daytime. SETTING: Experiments took place under controlled laboratory conditions. PARTICIPANTS: Twenty-seven healthy men aged between 18 and 30 years. MEASUREMENTS AND RESULTS: Compared with wakefulness, sleep was associated with a striking increase in the number of pre-mDC producing IL-12, which is a main inducer of Th1 responses. In addition, sleep slightly decreased PDC and also T cell counts but did not affect IFN-alpha production by PDC. Sleep, however, substantially decreased numbers of CD14(dim)CD16+ monocytes, probably reflecting increased margination of the cells upon a sleep-related drop in catecholamine release. CONCLUSIONS: Our data identify pre-mDC producing IL-12 as a basic target of sleep that is most closely related to mature APC function and whereby sleep can effectively enhance adaptive immune responses.

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Crossref · 10.1093/sleep/30.4.401

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  • There is converging evidence that sleep promotes Th1 immune responses as an immediate sequel of APC-T cell interactions.

    “There is converging evidence that sleep promotes Th1 immune responses as an immediate sequel of APC-T cell interactions.”

    From About Sleep's Role in Memory (2013) · cites this paper as [307]

    The discussion reports that sleep increased IL-12-producing dendritic-cell precursors and says this indicates a supportive influence on Th1-mediated adaptive immune responses. The introduction also explains that APCs can drive T cells toward type 1 responses. The paper does not establish that a Th1 response occurs as an immediate sequel of APC–T cell interactions; it presents that link as an inference from APC function. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • Sleep in humans enhances the production of IL-12 by monocytes and premyeloid dendritic cells (pre-mDC) which represent the most important precursors of mature professional APC circulating in blood.

    “Thus sleep in humans enhances the production of IL-12 by monocytes and premyolid dendritic cells (pre-mDC) which represent the most important precursors of mature professional APC circulating in blood (307, 696).”

    From About Sleep's Role in Memory (2013) · cites this paper as [307]

    The Results section reports that sleep increased the percentage of pre-mDC producing IL-12 after laboratory stimulation. The Discussion reports a comparable increase for monocytes in an earlier study, but says that result was variable and cautions that sleep does not affect monocytes globally. It also clarifies that the pre-mDC finding concerns capacity to produce IL-12 after stimulation, not spontaneous production in circulation. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • In vivo as well as in vitro studies identified high prolactin and low cortisol levels as factors most strongly contributing to the nocturnal upsurge of IL-12 production.

    “Moreover, in vivo as well as in vitro studies identified high prolactin and low cortisol levels as factors most strongly contributing to the nocturnal upsurge of IL-12 production (307, 696).”

    From About Sleep's Role in Memory (2013) · cites this paper as [307]

    The ‘Temporal Relationships Between Immune, Sleep and Hormonal Parameters’ section links higher IL-12 production during sleep to high prolactin and low cortisol, but also to low norepinephrine. The discussion describes these hormonal effects as possible mediators and cites earlier in vitro prolactin findings; it does not establish that prolactin and cortisol were the two factors most strongly contributing to the increase. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • In combination, these data support the view that sleep favors an accumulation of T cells in lymph nodes, and there might be a parallel effect on APC migration.

    “In combination, these data support the view that sleep favors an accumulation of T cells in lymph nodes, and there might be a parallel effect on APC migration (307, 627).”

    From About Sleep's Role in Memory (2013) · cites this paper as [307]

  • Stimulated TNF production in humans is acutely reduced during sleep compared with nocturnal wakefulness when measured in supernatant or intracellularly in CD8 T cells, but increased when measured in monocytes.

    “Stimulated production of TNF in humans has been found to be acutely reduced during sleep compared with nocturnal wakefulness when measured in the supernatant (552) or intracellularly in CD8 T cells (149), but increased when measured in monocytes (146).”

    From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [149]

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Research works printed but not linked: 1

  1. Fagnoni FF, Oliviero B, Zibera C et al. Circulating CD33+ large mononuclear cells contain three distinct populations with pheno- type of putative antigen-presenting cells including myeloid dendrit- ic cells and CD14+ monocytes with their CD16+ subset. Cytometry 2001;45:124-32.

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Authors
Dimitrov, S., Lange, T., Nohroudi, K., & Born, J.
Affiliations
  • University of Lübeck (DE)
  • University of Cologne (DE)
ORCID
Venue
Sleep
Publisher
Oxford University Press (OUP)
Published
2007-04
Volume
30
Issue
4
Pages
401-411
ISSN
  • 0161-8105 (ISSN-L)
  • 1550-9109
DOI
10.1093/sleep/30.4.401
OpenAlex
W2119664943
Cited by
164
References
45
Citations by year
2026: 4 · 2025: 13 · 2024: 19 · 2023: 10 · 2022: 12 · 2021: 14 · 2020: 9 · 2019: 10 · 2018: 1 · 2017: 8 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 7 · 2012: 9
FWCI
3.50
Type
journal article
Language
en
Open access
closed
Locations
  • SLEEP (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (SFB654)
  • Universität zu Lübeck
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
Keywords
circadian rhythm, dendritic cells, plasmacytoid dendritic cells, CD14+CD16+ monocytes, IL-12, interferon-alpha, antigen-presenting cells, adaptive immune response, Th1 response, catecholamine release, T-cell count, wakefulness
MeSH terms
Adolescent, Adult, Antigen-Presenting Cells, Circadian Rhythm, Humans, Immunity, Active, Immunologic Memory, Killer Cells, Natural, Leukocyte Count, Male, Monocytes, Reference Values, Sleep, Sleep Deprivation, Wakefulness, Interferon-alpha, Interleukin-12

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