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The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems
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tracrRNA is complementary to the repeat sequence and contains 3 stem-loop hairpin structures.
“tracrRNA is complementary to the repeat sequence and contains 3 stem-loop hairpin structures [35,36].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [35]
The Discussion (page not supplied) says tracrRNA contains an anti-repeat sequence that base-pairs with related pre-crRNA repeats, supporting the complementarity claim. Our copy does not establish that tracrRNA contains three stem-loop hairpin structures; searches for that structure and number found no supporting passage. (LLM, conf=0.88)
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Metadata
- Authors
- Chylinski, K., Le Rhun, A., & Charpentier, E.
- Venue
- RNA Biology
- Publisher
- Informa UK Limited
- Published
- 2013-04-05
- Volume
- 10
- Issue
- 5
- Pages
- 726-737
- ISSN
- 1547-6286
- 1555-8584
- PMC
- PMC3737331
- Cited by
- 353
- References
- 6
- Language
- en
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