The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
THE [ 14 C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT 1
Abstract
Abstract— A method has been developed for the simultaneous measurement of the rates of glucose consumption in the various structural and functional components of the brain in vivo. The method can be applied to most laboratory animals in the conscious state. It is based on the use of 2‐deoxy‐D‐[ 14 C]glucose ([ 14 C]DG) as a tracer for the exchange of glucose between plasma and brain and its phosphorylation by hexokinase in the tissues. [ 14 C]DG is used because the label in its product, [ 14 C]deoxyglucose‐6‐phosphate, is essentially trapped in the tissue over the time course of the measurement. A model has been designed based on the assumptions of a steady state for glucose consumption, a first order equilibration of the free [ 14 C]DG pool in the tissue with the plasma level, and relative rates of phosphorylation of [ 14 C]DG and glucose determined by their relative concentrations in the precursor pools and their respective kinetic constants for the hexokinase reaction. An operational equation based on this model has been derived in terms of determinable variables. A pulse of [ 14 C]DG is administered intravenously and the arterial plasma [ 14 C]DG and glucose concentrations monitored for a preset time between 30 and 45min. At the prescribed time, the head is removed and frozen in liquid N 2 ‐chilled Freon XII, and the brain sectioned for autoradiography. Local tissue concentrations of [ 14 C]DG are determined by quantitative autoradiography. Local cerebral glucose consumpt…
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“Lesional uptake of [18F]FDG is more or less irreversible in neoplastic cells [68,69] due to intracellular trapping of the phosphorylated molecule, although reversible uptake has been demonstrated in inflammatory tissue [18].”
From Influences on PET Quantification and Interpretation (2022) · cites this paper as [68]
In the citing paper
“18 F-fluoro-2-deoxyglucose (2-DG) (also known as FDG) is a radioactive glucose 2-DG analog that does not enter the glycolytic or Krebs cycle metabolic pathways in the brain (124, 397, 504, 552).”
From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [552]
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“Regions that gain volume correspond roughly to Swanson’s dorsolateral subsystem that mediates voluntary behaviors and cognition, whereas regions that lose volume correspond to the ventromedial subsystem that modulates affective and homeostatic functions45.”
From Magnetic resonance microscopy maps widespread effects of Alzheimer’s disease on brain structures and behavior in mice (2026) · cites this paper as [45]
Citations
Cites
(1975) · Journal of AOAC INTERNATIONAL · doi:10.1093/jaoac/58.5.1085 · Cited by 20,078
A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSE
Nelson, N. (1944) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)71980-7 · Cited by 10,376
Merritt, A. J. (1964) · Microchemical Journal · doi:10.1016/0026-265x(64)90139-0 · Cited by 5,180
Effect of Ischemia on Known Substrates and Cofactors of the Glycolytic Pathway in Brain
Lowry, O. H., Passonneau, J. V., Hasselberger, F. X., & Schulz, D. W. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)51740-3 · Cited by 2,222
Cerebral Blood Flow and Oxygen Consumption in Man
Lassen, N. A. (1959) · Physiological Reviews · doi:10.1152/physrev.1959.39.2.183 · Cited by 2,101
Kety, S. S., & Schmidt, C. F. (1948) · Journal of Clinical Investigation · doi:10.1172/jci101994 · Cited by 2,058
Brain uptake of radiolabeled amino acids, amines, and hexoses after arterial injection
Oldendorf, W. (1971) · American Journal of Physiology-Legacy Content · doi:10.1152/ajplegacy.1971.221.6.1629 · Cited by 1,691
MICRODETERMINATION OF OXYHEMOGLOBIN, METHEMOGLOBIN, AND SULFHEMOGLOBIN IN A SINGLE SAMPLE OF BLOOD
Evelyn, K. A., & Malloy, H. T. (1938) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)73873-8 · Cited by 1,163
Localization of the primary metabolic block produced by 2-deoxyglucose
Wick, A. N., Drury, D. R., Nakada, H. I., Wolfe, J. B., Britton, B., & Grabowski, R. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64988-9 · Cited by 780
SUBSTRATE SPECIFICITY OF BRAIN HEXOKINASE
Sols, A., & Crane, R. K. (1954) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)65384-0 · Cited by 599
Research works printed but not linked: 13
- Rosiers M. H., 1974, Neurology, 24, 389
- Dixon M., 1964, Enzymes, 84
- Hers H. G., 1950, Bull. Soc. chem. Biol., 32, 20
- Kennedy C., 1974, Trans. Am. Soc. Neurochem., 5, 86
- Kety S. S., 1960, Meth. Med. Res., 8, 228
- KnottG. D.&ReeceD. K.(1972) inProc. ONLINE ′72 International Conference Vol. 1 pp.497–526. Brunel University England.
- Landau W. H., 1955, Trans. Am. Neurol. Ass., 80, 125
- Plum F., 1976, Neurosci. Res. Prog. Bull., 14, 457
- Saifer A., 1958, J. Lab. Clin. Med., 51, 448
- Sokoloff L., 1960, Handbook of Physiology‐Neurophysiology, 1843
- Sokoloff L., 1969, Psychochemical Research in Man, 237
- Sokoloff L., 1974, Trans. Am. Soc. Neurochem., 5, 85
- Sokoloff L., 1976, Basic Neurochemistry, 388
Cited by
Sorted by citations
Items 1,001–1,025 of 1,025
Huchzermeyer, C., Berndt, N., Holzhütter, H.-G., & Kann, O. (2013) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2012.165 · Cited by 81
Levin, B. E. (2002) · Physiology & Behavior · doi:10.1016/s0031-9384(02)00763-1 · Cited by 79
Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo
Itoh, Y., Abe, T., Takaoka, R., & Tanahashi, N. (2004) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/01.wcb.0000127661.07591.de · Cited by 72
Functional Neuroimaging in Traumatic Brain Injury: From Nodes to Networks
Medaglia, J. D. (2017) · Frontiers in Neurology · doi:10.3389/fneur.2017.00407 · Cited by 63
GRUNSTEIN, H. S., JAMES, D. E., STORLIEN, L. H., SMYTHE, G. A., & KRAEGEN, E. W. (1985) · Endocrinology · doi:10.1210/endo-116-2-604 · Cited by 62
Interactions of Impaired Glucose Transport and Phosphorylation in Skeletal Muscle Insulin Resistance
Williams, K. V., Price, J. C., & Kelley, D. E. (2001) · Diabetes · doi:10.2337/diabetes.50.9.2069 · Cited by 61
Hippocampal hypometabolism in older adults with memory complaints and increased amyloid burden
Vannini, P., Hanseeuw, B., Munro, C. E., Amariglio, R. E., Marshall, G. A., Rentz, D. M., Pascual-Leone, A., Johnson, K. A., & Sperling, R. A. (2017) · Neurology · doi:10.1212/wnl.0000000000003889 · Cited by 61
Vyazovskiy, V. V., Cirelli, C., Tononi, G., & Tobler, I. (2008) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2007.10.040 · Cited by 60
Hertz, L., Gibbs, M. E., & Dienel, G. A. (2014) · Frontiers in Neuroscience · doi:10.3389/fnins.2014.00261 · Cited by 58
Dratman, M. B., Crutchfield, F. L., Futaesaku, Y., Goldberger, M. E., & Murray, M. (1987) · Journal of Comparative Neurology · doi:10.1002/cne.902600306 · Cited by 55
Intracellular ATP levels in mouse cortical excitatory neurons varies with sleep–wake states
Natsubori, A., Tsunematsu, T., Karashima, A., Imamura, H., Kabe, N., Trevisiol, A., Hirrlinger, J., Kodama, T., Sanagi, T., Masamoto, K., et al. (2020) · Communications Biology · doi:10.1038/s42003-020-01215-6 · Cited by 54
Insulin, Synaptic Function, and Opportunities for Neuroprotection
Mielke, J. G., & Wang, Y.-T. (2011) · Progress in Molecular Biology and Translational Science · doi:10.1016/b978-0-12-385506-0.00004-1 · Cited by 55
Maternal communication of circadian phase to the developing mammal
Reppert, S. (1988) · Psychoneuroendocrinology · doi:10.1016/0306-4530(88)90007-8 · Cited by 53
Phagocytic and metabolic reactions to chronically implanted metal brain electrodes.
Babb, T. L., & Kupfer, W. (1984) · Experimental Neurology · doi:10.1016/0014-4886(84)90179-1 · Cited by 51
Influences on PET Quantification and Interpretation
Rogasch, J. M. M., Hofheinz, F., van Heek, L., Voltin, C.-A., Boellaard, R., & Kobe, C. (2022) · Diagnostics · doi:10.3390/diagnostics12020451 · Cited by 57
“Lesional uptake of [18F]FDG is more or less irreversible in neoplastic cells [68,69] due to intracellular trapping of the phosphorylated molecule, although reversible uptake has been demonstrated in inflammatory tissue [18].”
BackgroundGjedde, A., Wong, D. F., Rosa-Neto, P., & Cumming, P. (2005) · International review of neurobiology · doi:10.1016/s0074-7742(05)63001-2 · Cited by 50
Benzodiazepine Dependence: From Neural Circuits to Gene Expression
Pratt, J. A., Brett, R. R., & Laurie, D. J. (1998) · Pharmacology Biochemistry and Behavior · doi:10.1016/s0091-3057(97)00539-x · Cited by 47
Advancing Our Understanding of the Glucose System via Modeling: A Perspective
Cobelli, C., Dalla Man, C., Pedersen, M. G., Bertoldo, A., & Toffolo, G. (2014) · IEEE Transactions on Biomedical Engineering · doi:10.1109/tbme.2014.2310514 · Cited by 46
Glucose and lactate as metabolic constraints on presynaptic transmission at an excitatory synapse
Lucas, S. J., Michel, C. B., Marra, V., Smalley, J. L., Hennig, M. H., Graham, B. P., & Forsythe, I. D. (2018) · The Journal of Physiology · doi:10.1113/jp275107 · Cited by 39
Spreading depression following experimental head injury in the rat
SUNAMI, K., NAKAMURA, T., KUBOTA, M., OZAWA, Y., NAMBA, H., YAMAURA, A., & MAKINO, H. (1989) · Neurologia medico-chirurgica · doi:10.2176/nmc.29.975 · Cited by 39
Dynamics of Neuronal and Astrocytic Energy Molecules in Epilepsy
Furukawa, K., Ikoma, Y., Niino, Y., Hiraoka, Y., Tanaka, K., Miyawaki, A., Hirrlinger, J., & Matsui, K. (2025) · Journal of Neurochemistry · doi:10.1111/jnc.70044 · Cited by 11
Volkow, N. D., Wang, G.-J., Begleiter, H., Porjesz, B., Fowler, J. S., Telang, F., Wong, C., Ma, Y., Logan, J., Goldstein, R., et al. (2006) · Archives of General Psychiatry · doi:10.1001/archpsyc.63.9.999 · Cited by 2
Tian, Y., Hornburg, K., Austin, W., Cook, J. J., Qi, Y., White, L. E., Killmar, J. T., Kaczorowski, C. C., Ashbrook, D. G., Johnson, G. A., et al. (2026) · Nature Neuroscience · doi:10.1038/s41593-025-02199-4 · Cited by 1
Lidow (1998) · doi:10.1002/(sici)1098-2396(199801)28:1
Metadata
- Authors
- Sokoloff, L., Reivich, M., Kennedy, C., Rosiers, M. H. D., Patlak, C. S., Pettigrew, K. D., Sakurada, O., & Shinohara, M.
- Affiliations
- National Institutes of Health (US)
- University of Pennsylvania (US)
- National Institute of Mental Health (US)
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1977-05
- Volume
- 28
- Issue
- 5
- Pages
- 897-916
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2090306035
- Cited by
- 6,000
- References
- 36
- Citations by year
- 2026: 22 · 2025: 37 · 2024: 45 · 2023: 44 · 2022: 64 · 2021: 60 · 2020: 78 · 2019: 63 · 2018: 70 · 2017: 67 · 2016: 73 · 2015: 68 · 2014: 97 · 2013: 95 · 2012: 101
- FWCI
- 22.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Medical Imaging Techniques and Applications
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- local cerebral glucose utilization, 14C-deoxyglucose, hexokinase, autoradiography, auditory cortex, medial geniculate body, superior olive, inferior colliculus, thiopental anesthesia, conscious rats, brain energy metabolism, regional cerebral blood flow
- MeSH terms
- Anesthesia, Animals, Antipyrine, Autoradiography, Blood Glucose, Brain, Deoxy Sugars, Deoxyglucose, Glucose, Hexokinase, Kinetics, Male, Mathematics, Methylglucosides, Models, Biological, Thiopental, Rats
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