Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

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Osawa, Masatake, Hanada, Ken-ichi, Hamada, Hirofumi, Nakauchi, Hiromitsu·1996·Science

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Abstract

Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34 lo/− , c-Kit + , Sca-1 + , lineage markers negative (Lin − ) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.

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Authors: Osawa, M., Hanada, K.-I., Hamada, H., & Nakauchi, H.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1996-07-12
Volume: 273
Issue: 5272
Pages: 242-245
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.273.5272.242
OpenAlex: W2083632015
Cited by: 2,140
References: 0
FWCI: 24.94
Type: journal article
Language: en
Open access: green

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Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

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