Cited by 410DNA Repair MechanismsCancer-related Molecular PathwaysBRCA gene mutations in cancer

DNA Damage-Induced Cell Cycle Checkpoint Control Requires CtIP, a Phosphorylation-Dependent Binding Partner of BRCA1 C-Terminal Domains

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Yu, Xiaochun, Chen, Junjie·2004·Molecular and Cellular Biology

Abstract

The BRCA1 C-terminal (BRCT) domain has recently been implicated as a phospho-protein binding domain. We demonstrate here that a CTBP-interacting protein CtIP interacts with BRCA1 BRCT domains in a phosphorylation-dependent manner. The CtIP/BRCA1 complex only exists in G(2) phase and is required for DNA damage-induced Chk1 phosphorylation and the G(2)/M transition checkpoint. However, the CtIP/BRCA1 complex is not required for the damage-induced G(2) accumulation checkpoint, which is controlled by a separate BRCA1/BACH1 complex. Taken together, these data not only implicate CtIP as a critical player in cell cycle checkpoint control but also provide molecular mechanisms by which BRCA1 controls multiple cell cycle transitions after DNA damage.

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Crossref · 10.1128/mcb.24.21.9478-9486.2004

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  • CTIP and ABRA1/Abraxas contain phospho-SXXF motifs that mediate direct interaction with BRCA1-BRCTs.

    “In addition to FANCJ, several proteins, including CTIP and ABRA1/Abraxas, have phospho-SXXF motifs (S is serine, F is phenylalanine and X varies) that mediate the direct interaction with the BRCA1-BRCTs, which are phospho-binding motifs [37–40].”

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Authors
Yu, X., & Chen, J.
Affiliations
Mayo Clinic (US)
Corresponding author
Chen, Junjie
Venue
Molecular and Cellular Biology
Published
2004-11-01
Volume
24
Issue
21
Pages
9478-9486
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2107991933
Cited by
410
References
40
Citations by year
2026: 7 · 2025: 10 · 2024: 10 · 2023: 15 · 2022: 12 · 2021: 17 · 2020: 19 · 2019: 15 · 2018: 15 · 2017: 24 · 2016: 22 · 2015: 25 · 2014: 30 · 2013: 28 · 2012: 27
FWCI
5.46
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Defense (DAMD17-02-1-0472)
  • Breast Cancer Research Foundation
  • Prospect Creek Foundation
  • National Institutes of Health
  • National Cancer Institute (R01CA089239, CA89239, R01CA092312, CA92312)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › BRCA gene mutations in cancer
Keywords
CtIP, BRCA1, BRCT domain, DNA damage response, cell cycle checkpoints, G2/M transition, Chk1 phosphorylation, G2 checkpoint, BACH1
MeSH terms
Checkpoint Kinase 1, Animals, Carrier Proteins, Cell Cycle, Cell Division, Cell Line, DNA Damage, Endodeoxyribonucleases, Enzyme Activation, Humans, Nuclear Proteins, Phosphorylation, Protein Binding, Protein Kinases, G2 Phase, Mutagenesis, Site-Directed, Protein Structure, Tertiary, BRCA1 Protein, RNA Interference, Multiprotein Complexes

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