DNA Damage-Induced Cell Cycle Checkpoint Control Requires CtIP, a Phosphorylation-Dependent Binding Partner of BRCA1 C-Terminal Domains
Open accessAbstract
The BRCA1 C-terminal (BRCT) domain has recently been implicated as a phospho-protein binding domain. We demonstrate here that a CTBP-interacting protein CtIP interacts with BRCA1 BRCT domains in a phosphorylation-dependent manner. The CtIP/BRCA1 complex only exists in G(2) phase and is required for DNA damage-induced Chk1 phosphorylation and the G(2)/M transition checkpoint. However, the CtIP/BRCA1 complex is not required for the damage-induced G(2) accumulation checkpoint, which is controlled by a separate BRCA1/BACH1 complex. Taken together, these data not only implicate CtIP as a critical player in cell cycle checkpoint control but also provide molecular mechanisms by which BRCA1 controls multiple cell cycle transitions after DNA damage.
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CTIP and ABRA1/Abraxas contain phospho-SXXF motifs that mediate direct interaction with BRCA1-BRCTs.
“In addition to FANCJ, several proteins, including CTIP and ABRA1/Abraxas, have phospho-SXXF motifs (S is serine, F is phenylalanine and X varies) that mediate the direct interaction with the BRCA1-BRCTs, which are phospho-binding motifs [37–40].”
From Hereditary Breast Cancer and the BRCA1-Associated FANCJ/BACH1/BRIP1 (2011) · cites this paper as [39]
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- Authors
- Yu, X., & Chen, J.
- Affiliations
- Mayo Clinic (US)
- Corresponding author
- Chen, Junjie
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2004-11-01
- Volume
- 24
- Issue
- 21
- Pages
- 9478-9486
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC522253
- OpenAlex
- W2107991933
- Cited by
- 410
- References
- 40
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 10 · 2023: 15 · 2022: 12 · 2021: 17 · 2020: 19 · 2019: 15 · 2018: 15 · 2017: 24 · 2016: 22 · 2015: 25 · 2014: 30 · 2013: 28 · 2012: 27
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Defense (DAMD17-02-1-0472)
- Breast Cancer Research Foundation
- Prospect Creek Foundation
- National Institutes of Health
- National Cancer Institute (R01CA089239, CA89239, R01CA092312, CA92312)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › BRCA gene mutations in cancer
- Keywords
- CtIP, BRCA1, BRCT domain, DNA damage response, cell cycle checkpoints, G2/M transition, Chk1 phosphorylation, G2 checkpoint, BACH1
- MeSH terms
- Checkpoint Kinase 1, Animals, Carrier Proteins, Cell Cycle, Cell Division, Cell Line, DNA Damage, Endodeoxyribonucleases, Enzyme Activation, Humans, Nuclear Proteins, Phosphorylation, Protein Binding, Protein Kinases, G2 Phase, Mutagenesis, Site-Directed, Protein Structure, Tertiary, BRCA1 Protein, RNA Interference, Multiprotein Complexes
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