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Cited by 829

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Evidence that production and release of amyloid beta-protein involves the endocytic pathway

Koo, E.H., Squazzo, S.L.·1994·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)32449-3
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Abstract

Amyloid beta-protein (A beta), the 40-43-amino acid polypeptide that is the principal constituent of senile plaques found in Alzheimer's disease, is constitutively produced and released into medium of cultured cells by an unclear mechanism. In this study, we report that one route of A beta generation involves the internalization of cell surface amyloid precursor protein (beta PP) via the coated pit-mediated endocytic pathway. Radiolabeled A beta can be recovered in medium following selective cell surface radioiodination, indicating that cell surface beta PP is a direct precursor to A beta. In addition, deletion of the cytoplasmic domains of beta PP or depletion of potassium in medium, both of which resulted in reduced beta PP internalization, significantly diminished A beta release. Moreover, pulse-chase experiments after surface radioiodination showed that the kinetics of beta PP secretion and A beta release was different, with the latter occurring at a significantly slower rate. We therefore hypothesize that the internalization of cell surface beta PP via coated pit-mediated endocytosis is one pathway leading to A beta generation and release into medium.

Overview

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Crossref · 10.1016/s0021-9258(17)32449-3

Cites

3 of 3 references verified0 citation errors · 0 not checkable

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Claims other papers make about this one

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  • Under physiological expression levels, β-secretase cleavage of wild-type APP has been localized predominantly to endosomal/lysosomal compartments.

    “whereas under physiological expression levels, ␤-secretase cleavage of wild-type APP has been localized predominantly to endosomal/lysosomal compartments (Koo and Squazzo, 1994;Haass et al., 1995;Thinakaran et al., 1996).”

    From GGA1 Is Expressed in the Human Brain and Affects the Generation of Amyloid β-Peptide (2006) · cites this paper as Koo and Squazzo, 1994

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 21

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Metadata

Authors
Koo, E. H., & Squazzo, S. L.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-07
Volume
269
Issue
26
Pages
17386-17389
ISSN
0021-9258
DOI
10.1016/s0021-9258(17)32449-3
Cited by
829
References
3
Language
en
Licence

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