Cited by 10,704Nitric Oxide and Endothelin EffectsEicosanoids and Hypertension PharmacologyCardiovascular Health and Disease Prevention

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Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor

Palmer, R. M. J., Ferrige, A. G., Moncada, S.·1987·Nature

DOI 10.1038/327524a0

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Abstract

Endothelium-derived relaxing factor (EDRF) is a labile humoral agent which mediates the action of some vasodilators. Nitrovasodilators, which may act by releasing nitric oxide (NO), mimic the effect of EDRF and it has recently been suggested by Furchgott that EDRF may be NO. We have examined this suggestion by studying the release of EDRF and NO from endothelial cells in culture. No was determined as the chemiluminescent product of its reaction with ozone. The biological activity of EDRF and of NO was measured by bioassay. The relaxation of the bioassay tissues induced by EDRF was indistinguishable from that induced by NO. Both substances were equally unstable. Bradykinin caused concentration-dependent release of NO from the cells in amounts sufficient to account for the biological activity of EDRF. The relaxations induced by EDRF and NO were inhibited by haemoglobin and enhanced by superoxide dismutase to a similar degree. Thus NO released from endothelial cells is indistinguishable from EDRF in terms of biological activity, stability, and susceptibility to an inhibitor and to a potentiator. We suggest that EDRF and NO are identical.

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1. Furchgott, R. F. in Mechanisms of Vasodilatation Vol. IV (ed. Vanhoutte, P. M.) (Raven, New York, in the press). 2. Downes, M. J., Edwards, M. W., Elsey, T. S. & Walters, C. L. Analyst 101, 742–748 (1976). 3. Gryglewski, R. J., Moncada, S. & Palmer, R. M. J. Br. J. Pharmac 87, 685–694 (1986). 4. Griffith, T. M., Edwards, D. H., Lewis, M. J., Newby, A. C. & Henderson, A. H. Nature 308, 645–647 (1984). 5. Forstermann, U., Trogisch, G. & Busse, R. Eur. J. Pharmac. 106, 639–643 (1985). 6. Cocks, T. M. & Angus, J. A. in Vascular Neuroeffector Mechanisms (eds Bevan, J. A., Godfraind, T., Maxwell, R. A., Stoclet, J. C. & Worcel, M.) 131–136 (Elsevier, Amsterdam, 1985). 7. Martin, W., Villani, G. M., Jothianandan, D. & Furchgott, R. F. / Pharmac. exp. Ther. 232, 708–716 (1985). 8. Gibson, Q. H & Roughton, F. J. W. J. Physiol., Lond. 136, 507–526 (1957). 9. Gryglewski, R. J., Palmer, R. M. J. & Moncada, S. Nature 320, 454–456 (1986). 10. Rubanyi, G. M & Vanhoutte, P. M. Am. J. Physiol. 250, H222–H227 (1986). 11. Dale, H. H. Hull. Johns Hopkins Hosp. 53, 297–347 (1933). 12. Moncada, S., Palmer, R. M. J. & Gryglewski, R. J. Proc. natn. Acad. Sci. U.S.A. 83,9164–9168 (1986). 13. Blough, N. V. & Zafiriou, O. C. Inorg. Chem. 24, 3502–3504 (1985).

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(1992) · doi:10.1126/science.1378650 · Cited by 1,163
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Johnstone, M. T., Creager, S. J., Scales, K. M., Cusco, J. A., Lee, B. K., & Creager, M. A. (1993) · Circulation · doi:10.1161/01.cir.88.6.2510 · Cited by 1,117
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Förstermann, U., Closs, E. I., Pollock, J. S., Nakane, M., Schwarz, P., Gath, I., & Kleinert, H. (1994) · Hypertension · doi:10.1161/01.hyp.23.6.1121 · Cited by 1,122
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Denninger, J. W., & Marletta, M. A. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00024-9 · Cited by 1,040
Gases as biological messengers: nitric oxide and carbon monoxide in the brain
Dawson, T., & Snyder, S. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-09-05147.1994 · Cited by 1,043
Release of endothelin from the porcine aorta. Inhibition by endothelium-derived nitric oxide.
Boulanger, C. M., & Lüscher, T. F. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114477 · Cited by 1,044
cGMP-Dependent Protein Kinases and cGMP Phosphodiesterases in Nitric Oxide and cGMP Action
Francis, S. H., Busch, J. L., & Corbin, J. D. (2010) · Pharmacological Reviews · doi:10.1124/pr.110.002907 · Cited by 1,059
Nitric oxide as a mediator of relaxation of the corpus cavernosum in response to nonadrenergic, noncholinergic neurotransmission
Rajfer, J., Aronson, W. J., Bush, P. A., Dorey, F. J., & Ignarro, L. J. (1992) · New England Journal of Medicine · doi:10.1056/nejm199201093260203 · Cited by 1,006
Impaired nitric oxide-mediated vasodilation in patients with non-insulin-dependent diabetes mellitus
Williams, S. B., Cusco, J. A., Roddy, M.-A., Johnstone, M. T., & Creager, M. A. (1996) · Journal of the American College of Cardiology · doi:10.1016/0735-1097(95)00522-6 · Cited by 1,014
Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability
Fukumura, D., Gohongi, T., Kadambi, A., Izumi, Y., Ang, J., Yun, C., Buerk, D. G., Huang, P. L., & Jain, R. K. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.041359198 · Cited by 1,027
Nitric Oxide: First in a New Class of Neurotransmitters
Snyder, S. H. (1992) · Science · doi:10.1126/science.1353273 · Cited by 1,004
Mammalian nitric oxide synthases
Stuehr, D. J. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00016-x · Cited by 996
Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter
Bult, H., Boeckxstaens, G. E., Pelckmans, P. A., Jordaens, F. H., Maercke, Y. M. V., & Herman, A. G. (1990) · Nature · doi:10.1038/345346a0 · Cited by 993
Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.
Pollock, J. S., Förstermann, U., Mitchell, J. A., Warner, T. D., Schmidt, H. H. H. W., Nakane, M., & Murad, F. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10480 · Cited by 979
Vasorelaxant properties of the endothelium-derived relaxing factor more closely resemble S-nitrosocysteine than nitric oxide
Myers, P. R., Minor, R. L., Guerra, R., Bates, J. N., & Harrison, D. G. (1990) · Nature · doi:10.1038/345161a0 · Cited by 973
Biological actions and properties of endothelium-derived nitric oxide formed and released from artery and vein.
Ignarro, L. J. (1989) · Circulation Research · doi:10.1161/01.res.65.1.1 · Cited by 964
Stroke protection by 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors mediated by endothelial nitric oxide synthase
Endres, M., Laufs, U., Huang, Z., Nakamura, T., Huang, P. L., Moskowitz, M. A., & Liao, J. K. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.15.8880 · Cited by 957
Endogenous nitric oxide is present in the exhaled air of rabbits, guinea pigs and humans
Gustafsson, L. E., Leone, A. M., Persson, M. G., Wiklund, N. P., & Moncada, S. (1991) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(91)91268-h · Cited by 964
Self-citation
Regular Physical Exercise Corrects Endothelial Dysfunction and Improves Exercise Capacity in Patients With Chronic Heart Failure
Hambrecht, R., Fiehn, E., Weigl, C., Gielen, S., Hamann, C., Kaiser, R., Yu, J., Adams, V., Niebauer, J., & Schuler, G. C. (1998) · Circulation · doi:10.1161/01.cir.98.24.2709 · Cited by 963
International Union of Pharmacology. XLV. Classification of the Kinin Receptor Family: from Molecular Mechanisms to Pathophysiological Consequences
Leeb-Lundberg, L. M. F., Marceau, F., Müller‐Esterl, W., Pettibone, D. J., & Zuraw, B. L. (2005) · Pharmacological Reviews · doi:10.1124/pr.57.1.2 · Cited by 956
“At the same time, the role of BK as a potent stimulator of endothelial nitric-oxide synthase (eNOS) via the B2 receptor signaling pathway was recognized (Palmer et al., 1987).”
Background
Impaired Neurogenic and Endothelium-Mediated Relaxation of Penile Smooth Muscle from Diabetic Men with Impotence
de Tejada, I. S., Goldstein, I., Azadzoi, K. M., Krane, R. J., & Cohen, R. A. (1989) · New England Journal of Medicine · doi:10.1056/nejm198904203201601 · Cited by 944

Authors: Palmer, R. M. J., Ferrige, A. G., & Moncada, S.
Affiliations: Wellcome Trust (GB)
ORCID: Palmer, R. M. J.
Moncada, S.
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 1987-06
Volume: 327
Issue: 6122
Pages: 524-526
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/327524a0
OpenAlex: W2007737411
Cited by: 10,704
References: 0
Citations by year: 2026: 60 · 2025: 81 · 2024: 91 · 2023: 124 · 2022: 138 · 2021: 131 · 2020: 134 · 2019: 143 · 2018: 146 · 2017: 165 · 2016: 160 · 2015: 193 · 2014: 211 · 2013: 227 · 2012: 260
FWCI: 210.31
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Health and Disease Prevention
Keywords: endothelium-derived relaxing factor, nitric oxide, endothelial cells, vasodilation, nitrovasodilators, bradykinin, superoxide dismutase, hemoglobin, chemiluminescence, ozone reactions
MeSH terms: Animals, Aorta, Bradykinin, Endothelium, Hemoglobins, Humans, Kinetics, Muscle Relaxation, Muscle, Smooth, Vascular, Nitric Oxide, Rabbits, Swine, Vasodilator Agents, In Vitro Techniques

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Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor

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