Quantitative profiling of gut microbiota of children with diarrhea-predominant irritable bowel syndrome
Abstract
OBJECTIVES: Human intestinal microbiota has a number of important roles in human health and is also implicated in several gastrointestinal disorders. The goal of this study was to determine the gut microbiota in two groups of pre- and adolescent children: healthy volunteers and children diagnosed with diarrhea predominant irritable bowel syndrome (IBS-D). METHODS: Phylogenetic Microbiota Array was used to obtain quantitative measurements of bacterial presence and abundance in subjects ’ fecal samples. We utilized high-throughput DNA sequencing, quantitative PCR, and fluorescent in situ hybridization to confirm microarray findings. RESULTS: Both sample groups were dominated by the phyla Firmicutes, Bacteroidetes, and Actinobacteria, which cumulatively constituted 91 % of overall sample composition on average. A core microbiome shared among analyzed samples encompassed 55 bacterial phylotypes dominated by genus Ruminococcus ; members of genera Clostridium , Faecalibacterium, Roseburia, Streptococcus , and Bacteroides were also present. Several genera were found to be differentially abundant in the gut of healthy and IBS groups: levels of Veillonella , Prevotella , Lactobacillus , and Parasporo bacterium were increased in children diagnosed with IBS, whereas members of Bifidobacterium and Verrucomicrobium were less abundant in those individuals. By calculating a nonparametric correlation matrix among abundances of different genera in all samples, we also examined potential associations among intestinal microbes. Strong positive correlations were found between abundances of Veillonella and both Haemophilus and Streptococcus , between Anaerovorax and Verrucomicrobium , and between Tannerella and Anaerophaga . CONCLUSIONS: Although at the higher taxonomical level gut microbiota was similar between healthy and IBS-D children, specific differences in the abundances of several bacterial genera were revealed. Core microbiome in children was dominated by Clostridia. Putative relationships identified among microbial genera provide testable hypotheses of cross-species associations among members of human gut microbiota
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In children ages 11-18, IBS-D is associated with increased abundance of Veillonella, Prevotella, Lactobacillus, and Parasporobacterium, and decreased abundance of Bifidobacterium and Verrucomicrobium.
“Another study of children ages 11–18 years found IBS-D to be associated with increased abundance of the genera Veillonella, Prevotella, Lactobacillus, and Parasporobacterium, and with decreased abundance of Bifidobacterium and Verrucomicrobium129.”
From The gut Microbiome in adult and pediatric functional gastrointestinal disorders (2019) · cites this paper as [129]
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“Our result was consistent with one study demonstrating that the level of Prevotella was increased in children diagnosed with IBS-D [26].”
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Self-citation
- Authors
- Rigsbee, L., Agans, R., Shankar, V., Kenche, H., Khamis, H. J., Michail, S., & Paliy, O.
- Affiliations
- Wright State University (US)
- Corresponding author
- Michail, Sonia
- Venue
- American Journal of Gastroenterology
- Published
- 2012-11
- Volume
- 107
- Issue
- 11
- Pages
- 1740-1751
- ISSN
- 0002-9270 (ISSN-L)
- OpenAlex
- W2017815964
- Cited by
- 207
- References
- 58
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 13 · 2023: 9 · 2022: 20 · 2021: 22 · 2020: 11 · 2019: 15 · 2018: 16 · 2017: 17 · 2016: 19 · 2015: 19 · 2014: 19 · 2013: 11 · 2012: 3
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The American Journal of Gastroenterology (publishedVersion)
- PubMed (publishedVersion)
- CORE Scholar (Wright State University) (submittedVersion)
- Journal of Bioresource Management (submittedVersion)
- Funders
- Ohio State University
- National Institutes of Health
- National Center for Complementary and Integrative Health (R21AT003423, R21AT003400, AT003400, AT003423)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD065575, R03HD065575)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- gut microbiota, diarrhea-predominant irritable bowel syndrome, pediatric IBS, core microbiome, bacterial abundance, Veillonella, Prevotella, Lactobacillus, Bifidobacterium, Clostridia, Firmicutes, Bacteroidetes, actinobacteria, microbial co-occurrence networks
- MeSH terms
- Adolescent, Bacteria, Child, Diarrhea, DNA, Bacterial, Female, Humans, Male, Genome, Bacterial, In Situ Hybridization, Fluorescence, Statistics, Nonparametric, Oligonucleotide Array Sequence Analysis, Irritable Bowel Syndrome, Real-Time Polymerase Chain Reaction
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