Effects of resveratrol on gut microbiota and fat storage in a mouse model with high-fat-induced obesity
Abstract
Recent studies have investigated the anti-obesity effect of resveratrol, but the pathways through which resveratrol resists obesity are not clear. In the present study, we hypothesize that resveratrol exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes, and in turn, improving fat storage and metabolism. Gut microbes, glucose and lipid metabolism in high-fat diet (HF) mice in vivo are investigated after resveratrol treatment. Several biochemical markers are measured. Fluorescence in situ hybridization and flow cytometry are used to monitor and quantify the changes in gut microbiota. The key genes related to fat storage and metabolism in the liver and visceral adipose tissues are measured by real-time PCR. The results show that resveratrol (200 mg per kg per day) significantly lowers both body and visceral adipose weights, and reduces blood glucose and lipid levels in HF mice. Resveratrol improves the gut microbiota dysbiosis induced by the HF diet, including increasing the Bacteroidetes-to-Firmicutes ratios, significantly inhibiting the growth of Enterococcus faecalis, and increasing the growth of Lactobacillus and Bifidobacterium. Furthermore, resveratrol significantly increases the fasting-induced adipose factor (Fiaf, a key gene negatively regulated by intestinal microbes) expression in the intestine. Resveratrol significantly decreases mRNA expression of Lpl, Scd1, Ppar-γ, Acc1, and Fas related to fatty acids synthesis, adipogenesis and lipogenesis, which may be driven by increased Fiaf expression. The Pearson's correlation coefficient shows that there is a negative correlation between the body weight and the ratios of Bacteroidetes-to-Firmicutes. Therefore, resveratrol mediates the composition of gut microbes, and in turn, through the Fiaf signaling pathway, accelerates the development of obesity.
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Resveratrol consumption can significantly modulate the growth of certain gut microbiota in vivo, including increasing the Bacteroidetes-to-Firmicutes ratio and the growth of Bacteroides, Lactobacillus, and Bifidobacterium.
“Moreover, it has been found that RSV consumption can significantly modulate the growth of certain gut microbiota in vivo, including increasing the Bacteroidetes-to-Firmicutes ratios, and the growth of Bacteroides, Lactobacillus, and Bifidobacterium (18–22).”
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- Authors
- Qiao, Y., Sun, J., Xia, S., Tang, X., Shi, Y., & Le, G.
- Affiliations
- Jiangnan University (CN)
- Corresponding author
- Le, Guowei
- Venue
- Food & Function
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2014
- Volume
- 5
- Issue
- 6
- Pages
- 1241
- ISSN
- 2042-6496 (ISSN-L)
- 2042-650X
- OpenAlex
- W2134140786
- Cited by
- 367
- References
- 47
- Citations by year
- 2026: 16 · 2025: 23 · 2024: 28 · 2023: 27 · 2022: 38 · 2021: 51 · 2020: 39 · 2019: 46 · 2018: 40 · 2017: 22 · 2016: 17 · 2015: 16 · 2014: 3
- FWCI
- 9.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food & Function (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- resveratrol, gut microbiota, high-fat diet-induced obesity, fat deposition, FIAF, Firmicutes/Bacteroidetes ratio, Enterococcus faecalis, Lactobacillus, Bifidobacterium, visceral adipose tissue, adipogenesis, lipogenesis, fatty acid synthesis, glucose metabolism, lipid metabolism, anti-obesity
- MeSH terms
- Angiopoietin-Like Protein 4, Resveratrol, Acetyl-CoA Carboxylase, Adipose Tissue, Animals, Bifidobacterium, Blood Glucose, Body Weight, Lactobacillus, Lipids, Liver, Male, Obesity, Stearoyl-CoA Desaturase, Stilbenes, Enterococcus faecalis, Signal Transduction, fas Receptor, Gastrointestinal Tract, Angiopoietins, PPAR gamma, Lipogenesis, Mice, Diet, High-Fat, Microbiota
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