Cited by 674Gut microbiota and healthAnaerobic Digestion and Biogas ProductionGastrointestinal motility and disorders

A humanized gnotobiotic mouse model of host-archaeal-bacterial mutualism

Samuel, Buck S., Gordon, Jeffrey I.·2006·Proceedings of the National Academy of Sciences

Abstract

Our colons harbor trillions of microbes including a prominent archaeon, Methanobrevibacter smithii. To examine the contributions of Archaea to digestive health, we colonized germ-free mice with Bacteroides thetaiotaomicron, an adaptive bacterial forager of the polysaccharides that we consume, with or without M. smithii or the sulfate-reducing bacterium Desulfovibrio piger. Whole-genome transcriptional profiling of B. thetaiotaomicron, combined with mass spectrometry, revealed that, unlike D. piger, M. smithii directs B. thetaiotaomicron to focus on fermentation of dietary fructans to acetate, whereas B. thetaiotaomicron-derived formate is used by M. smithii for methanogenesis. B. thetaiotaomicron-M. smithii cocolonization produces a significant increase in host adiposity compared with monoassociated, or B. thetaiotaomicron-D. piger biassociated, animals. These findings demonstrate a link between this archaeon, prioritized bacterial utilization of polysaccharides commonly encountered in our modern diets, and host energy balance.

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Crossref · 10.1073/pnas.0602187103

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Authors
Samuel, B. S., & Gordon, J. I.
Affiliations
Washington University in St. Louis (US)
Corresponding author
Gordon, Jeffrey I.
Venue
Proceedings of the National Academy of Sciences
Published
2006-06-27
Volume
103
Issue
26
Pages
10011-10016
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2068354067
Cited by
674
References
39
Citations by year
2026: 16 · 2025: 27 · 2024: 23 · 2023: 38 · 2022: 46 · 2021: 44 · 2020: 38 · 2019: 45 · 2018: 41 · 2017: 25 · 2016: 39 · 2015: 42 · 2014: 50 · 2013: 42 · 2012: 44
FWCI
8.30
Type
journal article
Language
en
Open access
closed
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation (0202737)
  • W. M. Keck Foundation
  • National Institutes of Health (DK70977)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R37DK030292, DK-30292, DK 70977, R01DK070977, R01DK030292)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
Methanobrevibacter smithii, Bacteroides thetaiotaomicron, Desulfovibrio piger, gnotobiotic mouse model, germ-free mice, gut microbiota, acetate production, formate, methanogenesis, sulfate reduction, polysaccharide utilization, adiposity, host-microbe interactions
MeSH terms
Animals, Bacteroides, Colon, Desulfovibrio, Diet, Digestion, Fermentation, Fructans, Germ-Free Life, Humans, Molecular Sequence Data, Polysaccharides, Symbiosis, Methanobrevibacter, Mice

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