A humanized gnotobiotic mouse model of host-archaeal-bacterial mutualism
Abstract
Our colons harbor trillions of microbes including a prominent archaeon, Methanobrevibacter smithii. To examine the contributions of Archaea to digestive health, we colonized germ-free mice with Bacteroides thetaiotaomicron, an adaptive bacterial forager of the polysaccharides that we consume, with or without M. smithii or the sulfate-reducing bacterium Desulfovibrio piger. Whole-genome transcriptional profiling of B. thetaiotaomicron, combined with mass spectrometry, revealed that, unlike D. piger, M. smithii directs B. thetaiotaomicron to focus on fermentation of dietary fructans to acetate, whereas B. thetaiotaomicron-derived formate is used by M. smithii for methanogenesis. B. thetaiotaomicron-M. smithii cocolonization produces a significant increase in host adiposity compared with monoassociated, or B. thetaiotaomicron-D. piger biassociated, animals. These findings demonstrate a link between this archaeon, prioritized bacterial utilization of polysaccharides commonly encountered in our modern diets, and host energy balance.
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Methanobrevibacter can increase short-chain fatty acid production by metabolizing hydrogen, a byproduct of bacterial carbohydrate fermentation.
“Methanobrevibacter can increase the production of short-chain fatty acids by metabolizing hydrogen, a byproduct of bacterial carbohydrate fermentation 48.”
From Diet and the Intestinal Microbiome: Associations, Functions, and Implications for Health and Disease (2014) · cites this paper as [48]
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- Authors
- Samuel, B. S., & Gordon, J. I.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Gordon, Jeffrey I.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2006-06-27
- Volume
- 103
- Issue
- 26
- Pages
- 10011-10016
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2068354067
- Cited by
- 674
- References
- 39
- Citations by year
- 2026: 16 · 2025: 27 · 2024: 23 · 2023: 38 · 2022: 46 · 2021: 44 · 2020: 38 · 2019: 45 · 2018: 41 · 2017: 25 · 2016: 39 · 2015: 42 · 2014: 50 · 2013: 42 · 2012: 44
- FWCI
- 8.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0202737)
- W. M. Keck Foundation
- National Institutes of Health (DK70977)
- National Institute of Diabetes and Digestive and Kidney Diseases (R37DK030292, DK-30292, DK 70977, R01DK070977, R01DK030292)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- Methanobrevibacter smithii, Bacteroides thetaiotaomicron, Desulfovibrio piger, gnotobiotic mouse model, germ-free mice, gut microbiota, acetate production, formate, methanogenesis, sulfate reduction, polysaccharide utilization, adiposity, host-microbe interactions
- MeSH terms
- Animals, Bacteroides, Colon, Desulfovibrio, Diet, Digestion, Fermentation, Fructans, Germ-Free Life, Humans, Molecular Sequence Data, Polysaccharides, Symbiosis, Methanobrevibacter, Mice
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