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Cited by 10,152

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY

Open access
GREENWOOD, FC, HUNTER, WM, GLOVER, JS·1963·Biochemical Journal
DOI 10.1042/bj0890114
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Abstract

A simple and rapid method is presented for the preparation of I/sup 131/- labeled human growth hormone of high specific radioactivity (240-300 mu C/ mu g). Low amounts of carrierfree I/sup 131/ iodide (2 mC) are allowed to react, without prior treatment, with small quantities of protein (5 mu g) in a highyield reaction (approx. 70% transfer of I/sup 131/ to protein). The degree of chemical substitution is minimized (0.5- 1.0 atom of iodine/molecule of protein) by the use of carrier-free I/sup 131/ iodide. The I/sup 131/-labeled hormone (up to 300 mu C/ mu g) contains no detectable degradation products and is immunologically identical with the unlabeled hormone. The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated. (auth)

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Metadata

Authors
GREENWOOD, F., HUNTER, W., & GLOVER, J.
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
1963-10-01
Volume
89
Issue
1
Pages
114-123
ISSN
0006-2936 (ISSN-L)
DOI
10.1042/bj0890114
OpenAlex
W1893651774
Cited by
10,152
References
1
FWCI
55.18
Type
journal article
Language
en
Open access
green

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