Cited by 999Neurogenesis and neuroplasticity mechanismsPluripotent Stem Cells ResearchDevelopmental Biology and Gene Regulation

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

Open access

Kadoshima, Taisuke, Sakaguchi, Hideya, Nakano, Tokushige, Soen, Mika, Ando, Satoshi, Eiraku, Mototsugu, Sasai, Yoshiki·2013·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1315710110PMC PMC3864329

Open this paper in CoChat — free account Open PDF

Abstract

Significance: Using 3D culture of human ES cells, we show new self-organizing aspects of human corticogenesis: spontaneous development of intracortical polarity, curving morphology, and complex zone separations. Moreover, this culture generates species-specific progenitors, outer radial glia, which are abundantly present in the human, but not mouse, neocortex. Our study suggests an unexpectedly wide range of self-organizing events that are driven by internal programs in human neocortex development.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1073/pnas.1315710110

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S.-I., Muguruma, K., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1310 · Cited by 2,055
Self-citation
Neuronal subtype specification in the cerebral cortex
Molyneaux, B. J., Arlotta, P., Menezes, J. R. L., & Macklis, J. D. (2007) · Nature reviews. Neuroscience · doi:10.1038/nrn2151 · Cited by 1,704
Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals
Eiraku, M., Watanabe, K., Matsuo-Takasaki, M., Kawada, M., Yonemura, S., Matsumura, M., Wataya, T., Nishiyama, A., Muguruma, K., & Sasai, Y. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.09.002 · Cited by 1,522
Self-citation
Self-formation of optic cups and storable stratified neural retina from human ESCs
Nakano, T., Ando, S., Takata, N., Kawada, M., Muguruma, K., Sekiguchi, K., Saito, K., Yonemura, S., Eiraku, M., & Sasai, Y. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.05.009 · Cited by 1,447
Self-citation
Neurogenic radial glia in the outer subventricular zone of human neocortex
Hansen, D. V., Lui, J. H., Parker, P. R. L., & Kriegstein, A. R. (2010) · Nature · doi:10.1038/nature08845 · Cited by 1,388
Neurons in Rhesus Monkey Visual Cortex: Systematic Relation between Time of Origin and Eventual Disposition
Rakic, P. (1974) · Science · doi:10.1126/science.183.4123.425 · Cited by 1,161
Development of the human cerebral cortex: Boulder Committee revisited
Bystron, I., Blakemore, C., & Rakic, P. (2008) · Nature Reviews Neuroscience · doi:10.1038/nrn2252 · Cited by 977
Developmental history of the transient subplate zone in the visual and somatosensory cortex of the macaque monkey and human brain
Kostović, I., & RAKIC, P. T. (1990) · The Journal of Comparative Neurology · doi:10.1002/cne.902970309 · Cited by 912
OSVZ progenitors of human and ferret neocortex are epithelial-like and expand by integrin signaling
Fietz, S. A., Kelava, I., Vogt, J., Wilsch-Bräuninger, M., Stenzel, D., Fish, J. L., Corbeil, D., Riehn, A., Distler, W., Nitsch, R., et al. (2010) · Nature Neuroscience · doi:10.1038/nn.2553 · Cited by 830

Show all 33 references

Research works printed but not linked: 2

SA Bayer, J Altman Atlas of Human Central Nervous System Development, Volume 3: The Human Brain During the Second Trimester (CRC Press, Boca Raton, FL, 2005).
SA Bayer, J Altman Atlas of Human Central Nervous System Development, Volume 2: The Human Brain During the Third Trimester (CRC Press, Boca Raton, FL, 2004).

Sorted by citations

Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,519
Organogenesis in a dish: Modeling development and disease using organoid technologies
Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931
Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure
Qian, X., Nguyen, H. N., Song, M. M., Hadiono, C., Ogden, S. C., Hammack, C., Yao, B., Hamersky, G. R., Jacob, F., Zhong, C., et al. (2016) · Cell · doi:10.1016/j.cell.2016.04.032 · Cited by 2,127
Generation of cerebral organoids from human pluripotent stem cells
Lancaster, M. A., & Knoblich, J. A. (2014) · Nature Protocols · doi:10.1038/nprot.2014.158 · Cited by 1,823
Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture
Paşca, A. M., Sloan, S. A., Clarke, L. E., Tian, Y., Makinson, C. D., Huber, N., Kim, C. H., Park, J.-Y., O'Rourke, N. A., Nguyen, K. D., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3415 · Cited by 1,609
Induced pluripotent stem cell technology: a decade of progress.
Shi, Y., Inoue, H., Wu, J. C., & Yamanaka, S. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.245 · Cited by 1,525
Assembly of functionally integrated human forebrain spheroids
Birey, F., Andersen, J., Makinson, C. D., Islam, S., Wei, W., Huber, N., Fan, H. C., Metzler, K. R. C., Panagiotakos, G., Thom, N., et al. (2017) · Nature · doi:10.1038/nature22330 · Cited by 1,365
An in vivo model of functional and vascularized human brain organoids
Mansour, A. A., Gonçalves, J. T., Bloyd, C. W., Li, H., Fernandes, S., Quang, D., Johnston, S., Parylak, S. L., Jin, X., & Gage, F. H. (2018) · Nature Biotechnology · doi:10.1038/nbt.4127 · Cited by 1,211
Engineering organoids
Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184
A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity
Jacob, F., Salinas, R. D., Zhang, D. Y., Nguyen, P. T. T., Schnoll, J. G., Wong, S. Z. H., Thokala, R., Sheikh, S., Saxena, D., Prokop, S., et al. (2020) · Cell · doi:10.1016/j.cell.2019.11.036 · Cited by 1,149
Individual brain organoids reproducibly form cell diversity of the human cerebral cortex
Velasco, S., Kedaigle, A. J., Simmons, S. K., Nash, A., Rocha, M., Quadrato, G., Paulsen, B., Nguyen, L., Adiconis, X., Regev, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1289-x · Cited by 1,094
Advancing the understanding of autism disease mechanisms through genetics
de la Torre-Ubieta, L., Won, H., Stein, J. L., & Geschwind, D. H. (2016) · Nature Medicine · doi:10.1038/nm.4071 · Cited by 827
The use of brain organoids to investigate neural development and disease
Di Lullo, E., & Kriegstein, A. R. (2017) · Nature Reviews Neuroscience · doi:10.1038/nrn.2017.107 · Cited by 788
Engineering Stem Cell Organoids
Yin, X., Mead, B. E., Safaee, H., Langer, R., Karp, J. M., & Levy, O. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2015.12.005 · Cited by 866
Fused cerebral organoids model interactions between brain regions
Bagley, J. A., Reumann, D., Bian, S., Lévi-Strauss, J., & Knoblich, J. A. (2017) · Nature Methods · doi:10.1038/nmeth.4304 · Cited by 790
Guided self-organization and cortical plate formation in human brain organoids
Lancaster, M. A., Corsini, N. S., Wolfinger, S., Gustafson, E. H., Phillips, A. W., Burkard, T. R., Otani, T., Livesey, F. J., & Knoblich, J. A. (2017) · Nature Biotechnology · doi:10.1038/nbt.3906 · Cited by 783
Brain organoids: advances, applications and challenges
Qian, X., Song, H., & Ming, G.-L. (2019) · Development · doi:10.1242/dev.166074 · Cited by 724
Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration
Xiang, Y., Tanaka, Y., Patterson, B., Kang, Y., Govindaiah, G., Roselaar, N., Çakır, B., Kim, K.-Y., Lombroso, A., Hwang, S., et al. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.07.007 · Cited by 711
Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output
Giandomenico, S. L., Mierau, S. B., Gibbons, G. M., Wenger, L. M. D., Masullo, L., Sit, T., Sutcliffe, M., Boulanger, J., Tripodi, M., Derivery, E., et al. (2019) · Nature Neuroscience · doi:10.1038/s41593-019-0350-2 · Cited by 651
Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution
Pollen, A. A., Bhaduri, A., Andrews, M. G., Nowakowski, T. J., Meyerson, O. S., Mostajo-Radji, M. A., Di Lullo, E., Alvarado, B., Bedolli, M., Dougherty, M. L., et al. (2019) · Cell · doi:10.1016/j.cell.2019.01.017 · Cited by 600
Cell stress in cortical organoids impairs molecular subtype specification
Bhaduri, A., Andrews, M. G., Mancia Leon, W., Jung, D., Shin, D., Allen, D., Jung, D., Schmunk, G., Haeussler, M., Salma, J., et al. (2020) · Nature · doi:10.1038/s41586-020-1962-0 · Cited by 624
Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia
Bershteyn, M., Nowakowski, T. J., Pollen, A. A., Di Lullo, E., Nene, A., Wynshaw-Boris, A., & Kriegstein, A. R. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2016.12.007 · Cited by 566
Self-Organizing 3D Human Neural Tissue Derived from Induced Pluripotent Stem Cells Recapitulate Alzheimer’s Disease Phenotypes
Raja, W. K., Mungenast, A. E., Lin, Y.-T., Ko, T., Abdurrob, F., Seo, J., & Tsai, L.-H. (2016) · PLOS ONE · doi:10.1371/journal.pone.0161969 · Cited by 569
A brief history of organoids
Corrò, C., Novellasdemunt, L., & Li, V. S. W. (2020) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00120.2020 · Cited by 561
Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue
Sakaguchi, H., Kadoshima, T., Soen, M., Narii, N., Ishida, Y., Ohgushi, M., Takahashi, J., Eiraku, M., & Sasai, Y. (2015) · Nature Communications · doi:10.1038/ncomms9896 · Cited by 542
Self-citation
Generation of human brain region–specific organoids using a miniaturized spinning bioreactor
Qian, X., Jacob, F., Song, M. M., Nguyen, H. N., Song, H., & Ming, G. (2018) · Nature Protocols · doi:10.1038/nprot.2017.152 · Cited by 528
Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids
Beccari, L. L., Moris, N., Girgin, M., Turner, D. A., Baillie‐Johnson, P., Cossy, A.-C., Lütolf, M. P., Duboule, D., & Arias, A. M. (2018) · Nature · doi:10.1038/s41586-018-0578-0 · Cited by 507
Generation and assembly of human brain region-specific three-dimensional cultures
Sloan, S. A., Andersen, J., Pașca, A. M., Birey, F., & Pașca, S. P. (2018) · Nature Protocols · doi:10.1038/s41596-018-0032-7 · Cited by 490
The rise of three-dimensional human brain cultures
Pașca, S. P. (2018) · Nature · doi:10.1038/nature25032 · Cited by 522
Human organoids in basic research and clinical applications
Tang, X.-Y., Wu, S., Wang, D., Chu, C., Hong, Y., Tao, M., Hu, H., Xu, M., Guo, X. L., & Liu, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01024-9 · Cited by 539
Sliced Human Cortical Organoids for Modeling Distinct Cortical Layer Formation
Qian, X., Su, Y., Adam, C. D., Deutschmann, A. U., Pather, S. R., Goldberg, E. M., Su, K., Li, S., Lu, L., Jacob, F., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2020.02.002 · Cited by 479
Outer Radial Glia-like Cancer Stem Cells Contribute to Heterogeneity of Glioblastoma
Bhaduri, A., Di Lullo, E., Jung, D., Müller, S., Crouch, E. E., Espinosa, C. S., Ozawa, T., Alvarado, B., Spatazza, J., Cadwell, C. R., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2019.11.015 · Cited by 419
Self‐organized developmental patterning and differentiation in cerebral organoids
Renner, M., Lancaster, M. A., Bian, S., Choi, H., Ku, T., Peer, A., Chung, K., & Knoblich, J. A. (2017) · The EMBO Journal · doi:10.15252/embj.201694700 · Cited by 389
Engineered materials for organoid systems
Kratochvil, M. J., Seymour, A. J., Li, T. L., Pașca, S. P., Kuo, C. J., & Heilshorn, S. C. (2019) · Nature Reviews Materials · doi:10.1038/s41578-019-0129-9 · Cited by 430
Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection
Watanabe, M., Buth, J. E., Vishlaghi, N., de la Torre-Ubieta, L., Taxidis, J., Khakh, B. S., Coppola, G., Pearson, C. A., Yamauchi, K., Gong, D., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.09.047 · Cited by 401
Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids
Cho, A.-N., Jin, Y., An, Y., Kim, J., Choi, Y. S., Lee, J. S., Kim, J., Choi, W.-Y., Koo, D.-J., Yu, W., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24775-5 · Cited by 397
Proliferation control in neural stem and progenitor cells
Homem, C. C. F., Repic, M., & Knoblich, J. A. (2015) · Nature reviews. Neuroscience · doi:10.1038/nrn4021 · Cited by 393
Generation of vascularized brain organoids to study neurovascular interactions
Sun, X.-Y., Ju, X.-C., Li, Y., Zeng, P.-M., Wu, J., Zhou, Y., Shen, L., Dong, J., Chen, Y., & Luo, Z. (2022) · eLife · doi:10.7554/elife.76707 · Cited by 404
2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size
Otani, T., Marchetto, M. C., Gage, F. H., Simons, B. D., & Livesey, F. J. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.03.003 · Cited by 353
Stem Cell Models of Human Brain Development
Kelava, I., & Lancaster, M. A. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.05.022 · Cited by 385
Disease modelling in human organoids
Lancaster, M. A., & Huch, M. (2019) · Disease Models & Mechanisms · doi:10.1242/dmm.039347 · Cited by 390
Induction of myelinating oligodendrocytes in human cortical spheroids
Madhavan, M., Nevin, Z. S., Shick, H. E., Garrison, E., Clarkson-Paredes, C., Karl, M., Clayton, B. L. L., Factor, D. C., Allan, K. C., Barbar, L., et al. (2018) · Nature Methods · doi:10.1038/s41592-018-0081-4 · Cited by 353
Brain organoids for the study of human neurobiology at the interface of in vitro and in vivo
Chiaradia, I., & Lancaster, M. A. (2020) · Nature Neuroscience · doi:10.1038/s41593-020-00730-3 · Cited by 360
Specification of positional identity in forebrain organoids
Cederquist, G., Asciolla, J. J., Tchieu, J. H., Walsh, R. M., Cornacchia, D., Resh, M. D., & Studer, L. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0085-3 · Cited by 334
Human brain organoid-on-a-chip to model prenatal nicotine exposure
Wang, Y., Wang, L., Zhu, Y., & Qin, J. (2018) · Lab on a Chip · doi:10.1039/c7lc01084b · Cited by 330
Building Models of Brain Disorders with Three-Dimensional Organoids
Amin, N. D., & Paşca, S. P. (2018) · Neuron · doi:10.1016/j.neuron.2018.10.007 · Cited by 312
Dishing out mini-brains: Current progress and future prospects in brain organoid research
Kelava, I., & Lancaster, M. A. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.06.037 · Cited by 323
How to make a midbrain dopaminergic neuron
Arenas, E., Denham, M., & Villaescusa, C. (2015) · Development · doi:10.1242/dev.097394 · Cited by 321
The promises and challenges of human brain organoids as models of neuropsychiatric disease
Quadrato, G., Brown, J., & Arlotta, P. (2016) · Nature Medicine · doi:10.1038/nm.4214 · Cited by 304
Human cerebral organoids — a new tool for clinical neurology research
Eichmüller, O. L., & Knoblich, J. A. (2022) · Nature Reviews Neurology · doi:10.1038/s41582-022-00723-9 · Cited by 319
Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development
Mora‐Bermúdez, F., Badsha, F., Kanton, S., Camp, J. G., Vernot, B., Köhler, K., Voigt, B., Okita, K., Maričić, T., He, Z., et al. (2016) · eLife · doi:10.7554/elife.18683 · Cited by 301
Generation and long-term culture of advanced cerebral organoids for studying later stages of neural development
Giandomenico, S. L., Sutcliffe, M. A., & Lancaster, M. A. (2020) · Nature Protocols · doi:10.1038/s41596-020-00433-w · Cited by 293
Single-cell epigenomics reveals mechanisms of human cortical development
Ziffra, R. S., Kim, C. N., Ross, J. M., Wilfert, A. B., Turner, T. N., Haeussler, M., Casella, A. M., Przytycki, P. F., Keough, K. C., Shin, D. S., et al. (2021) · Nature · doi:10.1038/s41586-021-03209-8 · Cited by 272
The Contribution of Microglia to Neuroinflammation in Parkinson’s Disease
Badanjak, K., Fixemer, S., Smajić, S., Skupin, A., & Grünewald, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22094676 · Cited by 296
An Organoid-Based Model of Cortical Development Identifies Non-Cell-Autonomous Defects in Wnt Signaling Contributing to Miller-Dieker Syndrome
Iefremova, V., Manikakis, G., Krefft, O., Jabali, A., Weynans, K., Wilkens, R., Marsoner, F., Brändl, B., Müller, F.-J., Koch, P., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.03.047 · Cited by 261
An early cell shape transition drives evolutionary expansion of the human forebrain
Benito-Kwiecinski, S. K., Giandomenico, S. L., Sutcliffe, M. A., Riis, E. S., Freire-Pritchett, P., Kelava, I., Wunderlich, S., Martin, U., Wray, G. A., McDole, K., et al. (2021) · Cell · doi:10.1016/j.cell.2021.02.050 · Cited by 272
3D neural tissue models: From spheroids to bioprinting
Zhuang, P., Sun, A. X., An, J., Chua, C. K., & Chew, S. Y. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.10.002 · Cited by 269
Evaluation of variability in human kidney organoids
Phipson, B., Er, P. X., Combes, A. N., Forbes, T. A., Howden, S. E., Zappia, L., Yen, H.-J., Lawlor, K. T., Hale, L. J., Sun, J. Q. J., et al. (2018) · Nature Methods · doi:10.1038/s41592-018-0253-2 · Cited by 264
Challenges of Organoid Research
Andrews, M. G., & Kriegstein, A. R. (2022) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-111020-090812 · Cited by 270
A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity
Pamies, D. (2016) · ALTEX · doi:10.14573/altex.1609122 · Cited by 266
Synthetic Analyses of Single-Cell Transcriptomes from Multiple Brain Organoids and Fetal Brain
Tanaka, Y., Çakır, B., Xiang, Y., Sullivan, G. J., & Park, I. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.01.038 · Cited by 244
Using brain organoids to understand Zika virus-induced microcephaly
Qian, X., Nguyen, H. N., Jacob, F., Song, H., & Ming, G. (2017) · Development · doi:10.1242/dev.140707 · Cited by 248
Human brain organogenesis: Toward a cellular understanding of development and disease
Kelley, K. W., & Pașca, S. P. (2021) · Cell · doi:10.1016/j.cell.2021.10.003 · Cited by 244
Non-model model organisms
Russell, J. J., Theriot, J. A., Sood, P., Marshall, W. F., Landweber, L. F., Fritz‐Laylin, L. K., Polka, J., Oliferenko, S., Gerbich, T. M., Gladfelter, A. S., et al. (2017) · BMC Biology · doi:10.1186/s12915-017-0391-5 · Cited by 237
A Quantitative Framework to Evaluate Modeling of Cortical Development by Neural Stem Cells
Stein, J. L., de la Torre-Ubieta, L., Tian, Y., Parikshak, N. N., Hernández, I. A., Marchetto, M. C., Baker, D. K., Lu, D., Hinman, C. R., Lowe, J. K., et al. (2014) · Neuron · doi:10.1016/j.neuron.2014.05.035 · Cited by 201
Bioengineering Approaches for the Advanced Organoid Research
Yi, S. A., Zhang, Y., Rathnam, C., Pongkulapa, T., & Lee, K. (2021) · Advanced Materials · doi:10.1002/adma.202007949 · Cited by 218
The ethics of experimenting with human brain tissue
Farahany, N. A., Greely, H. T., Hyman, S. E., Koch, C., Grady, C., Pașca, S. P., Šestan, N., Arlotta, P., Bernat, J. L., Ting, J. T., et al. (2018) · Nature · doi:10.1038/d41586-018-04813-x · Cited by 223
Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
Wang, Y., Wang, L., Guo, Y., Zhu, Y., & Qin, J. (2018) · RSC Advances · doi:10.1039/c7ra11714k · Cited by 201
Embryoids, organoids and gastruloids: new approaches to understanding embryogenesis
Simunovic, M., & Brivanlou, A. H. (2017) · Development · doi:10.1242/dev.143529 · Cited by 204
3D brain Organoids derived from pluripotent stem cells: promising experimental models for brain development and neurodegenerative disorders
Lee, C., Bendriem, R. M., Wu, W. W., & Shen, R. (2017) · Journal of Biomedical Science · doi:10.1186/s12929-017-0362-8 · Cited by 208
Three-dimensional induction of dorsal, intermediate and ventral spinal cord tissues from human pluripotent stem cells
Ogura, T., Sakaguchi, H., Miyamoto, S., & Takahashi, J. (2018) · Development · doi:10.1242/dev.162214 · Cited by 183
Self-citation
Use and application of 3D-organoid technology
Artegiani, B., & Clevers, H. C. (2018) · Human Molecular Genetics · doi:10.1093/hmg/ddy187 · Cited by 198
Human Models Are Needed for Studying Human Neurodevelopmental Disorders
Zhao, X., & Bhattacharyya, A. (2018) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2018.10.009 · Cited by 192
Modeling microcephaly with cerebral organoids reveals a WDR62–CEP170–KIF2A pathway promoting cilium disassembly in neural progenitors
Zhang, W., Yang, S.-L., Yang, M., Herrlinger, S. A., Shao, Q., Collar, J. L., Fierro, E., Shi, Y., Liu, A., Lü, H., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10497-2 · Cited by 195
Programming and Reprogramming Cellular Age in the Era of Induced Pluripotency
Studer, L., Vera, E., & Cornacchia, D. (2015) · Cell stem cell · doi:10.1016/j.stem.2015.05.004 · Cited by 192
Modeling Neurological Diseases With Human Brain Organoids
Wang, H. (2018) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2018.00015 · Cited by 181
Developmental mechanisms underlying the evolution of human cortical circuits
Vanderhaeghen, P., & Polleux, F. (2023) · Nature Reviews Neuroscience · doi:10.1038/s41583-023-00675-z · Cited by 173
Production of human spinal-cord organoids recapitulating neural-tube morphogenesis
Lee, J., Shin, H., Shaker, M. R., Kim, H. J., Park, S.-H., Kim, J. H., Lee, N., Kang, M., Cho, S., Kwak, T. H., et al. (2022) · Nature Biomedical Engineering · doi:10.1038/s41551-022-00868-4 · Cited by 179
Brain organoids: an ensemble of bioassays to investigate human neurodevelopment and disease
Sidhaye, J., & Knoblich, J. A. (2020) · Cell Death and Differentiation · doi:10.1038/s41418-020-0566-4 · Cited by 170
SOX Transcription Factors as Important Regulators of Neuronal and Glial Differentiation During Nervous System Development and Adult Neurogenesis
Stevanović, M. J., Drakulić, D., Lazic, A., Ninković, D. S., Schwirtlich, M., & Mojsin, M. M. (2021) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2021.654031 · Cited by 168
Human induced pluripotent stem cells for modelling neurodevelopmental disorders
Ardhanareeswaran, K., Mariani, J., Coppola, G., Abyzov, A. S., & Vaccarino, F. M. (2017) · Nature Reviews Neurology · doi:10.1038/nrneurol.2017.45 · Cited by 162
Vascularization of Human Brain Organoids
Matsui, T. K., Tsuru, Y., Hasegawa, K., & Kuwako, K.-I. (2021) · Stem Cells · doi:10.1002/stem.3368 · Cited by 163
Single-cell transcriptomics captures features of human midbrain development and dopamine neuron diversity in brain organoids
Fiorenzano, A., Sozzi, E., Birtele, M., Kajtez, J., Giacomoni, J., Nilsson, F., Bruzelius, A., Sharma, Y., Zhang, Y., Mattsson, B., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-27464-5 · Cited by 160
Recapitulating cortical development with organoid culture in vitro and modeling abnormal spindle-like (ASPM related primary) microcephaly disease
Li, R., Sun, L., Fang, A., Li, P., Wu, Q., & Wang, X. (2017) · Protein & Cell · doi:10.1007/s13238-017-0479-2 · Cited by 158
A human three-dimensional neural-perivascular ‘assembloid’ promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology
Wang, L., Sievert, D., Clark, A. E., Lee, S., Federman, H. G., Gastfriend, B. D., Shusta, E. V., Palecek, S. P., Carlin, A. F., & Gleeson, J. G. (2021) · Nature Medicine · doi:10.1038/s41591-021-01443-1 · Cited by 158
mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex
Andrews, M. G., Subramanian, L., & Kriegstein, A. R. (2020) · eLife · doi:10.7554/elife.58737 · Cited by 148
3D heterogeneous islet organoid generation from human embryonic stem cells using a novel engineered hydrogel platform
Candiello, J., Grandhi, T. S. P., Goh, S. K., Vaidya, V., Lemmon-Kishi, M., Eliato, K. R., Ros, R., Kumta, P. N., Rege, K., & Banerjee, I. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.05.031 · Cited by 146
Human iPSC‐derived microglia: A growing toolset to study the brain's innate immune cells
Hasselmann, J., & Blurton‐Jones, M. (2020) · Glia · doi:10.1002/glia.23781 · Cited by 145
Modelling human brain development and disease with organoids
Birtele, M., Lancaster, M. A., & Quadrato, G. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00804-1 · Cited by 177
Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium
Lindborg, B. A., Brekke, J. H., Vegoe, A. L., Ulrich, C. B., Haider, K. T., Subramaniam, S., Venhuizen, S. L., Eide, C. R., Orchard, P. J., Chen, W., et al. (2016) · Stem Cells Translational Medicine · doi:10.5966/sctm.2015-0305 · Cited by 141
Cerebral organoid and mouse models reveal a RAB39b–PI3K–mTOR pathway-dependent dysregulation of cortical development leading to macrocephaly/autism phenotypesPreprint
Zhang, W., Ma, L., Yang, M., Shao, Q., Xu, J., Lu, Z., Zhao, Z., Chen, R., Chai, Y., & Chen, J. (2020) · Genes & Development · doi:10.1101/gad.332494.119 · Cited by 143
Stem cell models of Alzheimer’s disease: progress and challenges
Arber, C., Lovejoy, C., & Wray, S. (2017) · Alzheimer's Research & Therapy · doi:10.1186/s13195-017-0268-4 · Cited by 143
DSCAM/PAK1 pathway suppression reverses neurogenesis deficits in iPSC-derived cerebral organoids from patients with Down syndrome
Tang, X.-Y., Xu, L., Wang, J., Hong, Y., Wang, Y., Zhu, Q., Wang, D., Zhang, X.-Y., Liu, C.-Y., Fang, K.-H., et al. (2021) · Journal of Clinical Investigation · doi:10.1172/jci135763 · Cited by 143
Stem Cells, Genome Editing, and the Path to Translational Medicine
Soldner, F., & Jaenisch, R. (2018) · Cell · doi:10.1016/j.cell.2018.09.010 · Cited by 144
Applications of Human Brain Organoids to Clinical Problems
Chen, H.-C. I., Song, H., & Ming, G. (2018) · Developmental Dynamics · doi:10.1002/dvdy.24662 · Cited by 147
In situ generation of human brain organoids on a micropillar array
Zhu, Y., Wang, L., Yu, H., Yin, F., Wang, Y., Liu, H., Jiang, L., & Qin, J. (2017) · Lab on a Chip · doi:10.1039/c7lc00682a · Cited by 144
CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma
Liu, S. J., Malatesta, M., Lien, B. V., Saha, P., Thombare, S. S., Hong, S. J., Pedraza, L., Koontz, M., Seo, K., Horlbeck, M. A., et al. (2020) · Genome biology · doi:10.1186/s13059-020-01995-4 · Cited by 144
Brain Organoids and the Study of Neurodevelopment
Trujillo, C. A., & Muotri, A. R. (2018) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2018.09.005 · Cited by 139
The Ethics of Cerebral Organoid Research: Being Conscious of Consciousness
Sawai, T., Sakaguchi, H., Thomas, E. S., Takahashi, J. B., & Fujita, M. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.08.003 · Cited by 140
Self-citation
Structural and functional integration of human forebrain organoids with the injured adult rat visual system
Jgamadze, D., Lim, J. T., Zhang, Z., Harary, P. M., Germi, J., Mensah-Brown, K. G., Adam, C. D., Mirzakhalili, E., Singh, S., Gu, J. B., et al. (2023) · Cell stem cell · doi:10.1016/j.stem.2023.01.004 · Cited by 138

Authors: Kadoshima, T., Sakaguchi, H., Nakano, T., Soen, M., Ando, S., Eiraku, M., & Sasai, Y.
Affiliations: Kyoto University (JP)
Laboratory of Organogenesis and Neurogenesis and
RIKEN (JP)
ORCID: Sakaguchi, Hideya
Ando, Satoshi
Eiraku, Mototsugu
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2013-11-25
Volume: 110
Issue: 50
Pages: 20284-20289
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1315710110
PMC: PMC3864329
OpenAlex: W2051785391
Cited by: 999
References: 33
Citations by year: 2026: 62 · 2025: 99 · 2024: 90 · 2023: 84 · 2022: 122 · 2021: 113 · 2020: 113 · 2019: 94 · 2018: 66 · 2017: 66 · 2016: 44 · 2015: 30 · 2014: 16
FWCI: 11.13
Type: journal article
Language: en
Update notices: correction (2014-04-29)
Open access: bronze
doi.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Kyoto University Research Information Repository (Kyoto University) (submittedVersion)
Funders: Ministry of Education, Culture, Sports, Science and Technology
Japan Society for the Promotion of Science (24680037)
Japan Science and Technology Agency
Topics: Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: human embryonic stem cells, self-organization, axial polarity, subplate, cortical plate, outer subventricular zone, outer radial glia, 3D cell culture, cortical morphogenesis
MeSH terms: Amides, Collagen, Drug Combinations, Humans, Laminin, Neuroglia, Proteoglycans, Pyridines, Species Specificity, Cell Polarity, Cell Culture Techniques, Neocortex, Organogenesis, Embryonic Stem Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks