The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Clathrin-mediated endocytosis in AP-2–depleted cells
Open accessAbstract
We have used RNA interference to knock down the AP-2 μ2 subunit and clathrin heavy chain to undetectable levels in HeLaM cells. Clathrin-coated pits associated with the plasma membrane were still present in the AP-2–depleted cells, but they were 12-fold less abundant than in control cells. No clathrin-coated pits or vesicles could be detected in the clathrin-depleted cells, and post-Golgi membrane compartments were swollen. Receptor-mediated endocytosis of transferrin was severely inhibited in both clathrin- and AP-2–depleted cells. Endocytosis of EGF, and of an LDL receptor chimera, were also inhibited in the clathrin-depleted cells; however, both were internalized as efficiently in the AP-2–depleted cells as in control cells. These results indicate that AP-2 is not essential for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins including the transferrin receptor. Uptake of the EGF and LDL receptors may be facilitated by alternative adaptors.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Curvature of clathrin-coated pits driven by epsin
Ford, M. G. J., Mills, I. G., Peter, B. J., Vallis, Y., Praefcke, G. J. K., Evans, P. R., & McMahon, H. T. (2002) · Nature · doi:10.1038/nature01020 · Cited by 1,019
Disabled‐2 exhibits the properties of a cargo‐selective endocytic clathrin adaptor
Mishra, S. K., Keyel, P. A., Hawryluk, M. J., Agostinelli, N. R., Watkins, S. C., & Traub, L. M. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf487 · Cited by 302
Cited by
Molecular mechanism and physiological functions of clathrin-mediated endocytosis
McMahon, H. T., & Boucrot, E. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3151 · Cited by 2,223
Mercer, J., Schelhaas, M., & Helenius, A. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060208-104626 · Cited by 1,057
α-Synuclein impairs macroautophagy: implications for Parkinson’s disease
Winslow, A. R., Chen, C.-W., Corrochano, S., Acevedo-Arozena, A., Gordon, D. E., Peden, A. A., Lichtenberg, M., Menzies, F. M., Ravikumar, B., Imarisio, S., et al. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201003122 · Cited by 825
“1035-Synuclein impairs macroautophagy • Winslow et al. For siRNA experiments, C1 cells were transfected with OnTargetPlus siRNA oligonucleotides (100 nM final concentration; Thermo Fisher Scientific) using Oligofectamine (Invitrogen) as previously described (Motley et al., 2003).”
EGF receptor trafficking: consequences for signaling and cancer
Tomas, A., Futter, C. E., & Eden, E. R. (2014) · Trends in Cell Biology · doi:10.1016/j.tcb.2013.11.002 · Cited by 779
Clathrin-independent endocytosis of ubiquitinated cargos
Sigismund, S., Woelk, T., Puri, C., Maspero, E., Tacchetti, C., Transidico, P., Di Fiore, P. P., & Polo, S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409817102 · Cited by 739
Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
Seaman, M. N. J. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200312034 · Cited by 624
Self-citationEndophilin marks and controls a clathrin-independent endocytic pathway
Boucrot, E., Ferreira, A. P. A., Almeida-Souza, L., Debard, S., Vallis, Y., Howard, G., Bertot, L., Sauvonnet, N., & McMahon, H. T. (2015) · Nature · doi:10.1038/nature14067 · Cited by 542
Lakadamyali, M., Rust, M. J., & Zhuang, X. (2006) · Cell · doi:10.1016/j.cell.2005.12.038 · Cited by 508
FCHo Proteins Are Nucleators of Clathrin-Mediated Endocytosis
Henne, W. M., Boucrot, E., Meinecke, M., Evergren, E., Vallis, Y., Mittal, R., & McMahon, H. T. (2010) · Science · doi:10.1126/science.1188462 · Cited by 483
The intracellular trafficking pathway of transferrin
Mayle, K. M., Le, A. M., & Kamei, D. T. (2012) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2011.09.009 · Cited by 466
Endocytosis and signaling: Cell logistics shape the eukaryotic cell plan
Sigismund, S., Confalonieri, S., Ciliberto, A., Polo, S., Scita, G., & Di Fiore, P. P. (2012) · Physiological Reviews · doi:10.1152/physrev.00005.2011 · Cited by 338
Integrating molecular and network biology to decode endocytosis
Schmid, E. M., & McMahon, H. T. (2007) · Nature · doi:10.1038/nature06031 · Cited by 315
Traub (2003) · doi:10.1083/jcb.200309175 · Cited by 290
Clathrin-mediated endocytosis in budding yeast
Weinberg, J., & Drubin, D. G. (2012) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.09.001 · Cited by 236
Mitchell, H., Choudhury, A., Pagano, R. E., & Leof, E. B. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-03-0245 · Cited by 215
Clathrin-independent pathways do not contribute significantly to endocytic flux
Bitsikas, V., Corrêa, I. R., & Nichols, B. J. (2014) · eLife · doi:10.7554/elife.03970 · Cited by 199
Miller, S. E., Mathiasen, S., Bright, N. A., Pierre, F., Kelly, B. T., Kladt, N., Schauss, A., Merrifield, C. J., Stamou, D., Höning, S., et al. (2015) · Developmental Cell · doi:10.1016/j.devcel.2015.03.002 · Cited by 207
Self-citationA genome-wide atlas of co-essential modules assigns function to uncharacterized genes
Wainberg, M., Kamber, R. A., Balsubramani, A., Meyers, R. M., Sinnott-Armstrong, N., Hornburg, D., Jiang, L., Chan, J., Jian, R., Gu, M., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00840-z · Cited by 204
Differential requirements for AP-2 in clathrin-mediated endocytosis
Conner, S. D., & Schmid, S. L. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200304069 · Cited by 196
The adaptor protein Dab2 sorts LDL receptors into coated pits independently of AP-2 and ARH
Maurer, M. E., & Cooper, J. A. (2006) · Journal of Cell Science · doi:10.1242/jcs.03217 · Cited by 195
The Molecular Basis for the Endocytosis of Small R-SNAREs by the Clathrin Adaptor CALM
Miller, S. E., Sahlender, D. A., Graham, S. C., Höning, S., Robinson, M. S., Peden, A. A., & Owen, D. J. (2011) · Cell · doi:10.1016/j.cell.2011.10.038 · Cited by 192
Self-citationEvolving nature of the AP2 α‐appendage hub during clathrin‐coated vesicle endocytosis
Praefcke, G. J., Ford, M. G., Schmid, E. M., Olesen, L. E., Gallop, J. L., Peak‐Chew, S., Vallis, Y., Babu, M. M., Mills, I. G., & McMahon, H. T. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600445 · Cited by 188
Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus
Chen, C., & Zhuang, X. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0803711105 · Cited by 186
Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins
Badgandi, H. B., Hwang, S.-H., Shimada, I. S., Loriot, E., & Mukhopadhyay, S. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201607095 · Cited by 182
Cargo- and adaptor-specific mechanisms regulate clathrin-mediated endocytosis
Mettlen, M., Loerke, D., Yarar, D., Danuser, G., & Schmid, S. L. (2010) · Journal of Cell Biology · doi:10.1083/jcb.200908078 · Cited by 177
Defects in ER–endosome contacts impact lysosome function in hereditary spastic paraplegia
Allison, R., Edgar, J. R., Pearson, G., Rizo, T., Newton, T., Günther, S., Berner, F., Hague, J., Connell, J. W., Winkler, J., et al. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201609033 · Cited by 171
Bowers, K., Piper, S. C., Edeling, M. A., Gray, S. R., Owen, D. J., Lehner, P. J., & Luzio, J. P. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m508632200 · Cited by 166
Endocytosis in the context-dependent regulation of individual and collective cell properties
Sigismund, S., Lanzetti, L., Scita, G., & Di Fiore, P. P. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00375-5 · Cited by 132
Keyel, P. A., Mishra, S. K., Roth, R., Heuser, J. E., Watkins, S. C., & Traub, L. M. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-05-0421 · Cited by 134
Park, M., Song, K., Reichardt, I., Kim, H., Mayer, U., Stierhof, Y.-D., Hwang, I., & Jürgens, G. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1300460110 · Cited by 134
EHD1 Interacts with Retromer to Stabilize SNX1 Tubules and Facilitate Endosome‐to‐Golgi Retrieval
Gokool, S., Tattersall, D., & Seaman, M. N. J. (2007) · Traffic · doi:10.1111/j.1600-0854.2007.00652.x · Cited by 128
Self-citationRevisiting the Role of Clathrin-Mediated Endoytosis in Synaptic Vesicle Recycling
Milosevic, I. (2018) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2018.00027 · Cited by 122
Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network
Carreno, S., Engqvist-Goldstein, A. E., Zhang, C. X., McDonald, K. L., & Drubin, D. G. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200403120 · Cited by 118
Transient Fcho1/2⋅Eps15/R⋅AP-2 Nanoclusters Prime the AP-2 Clathrin Adaptor for Cargo Binding
Ma, L., Umasankar, P. K., Wrobel, A. G., Lymar, A., McCoy, A. J., Holkar, S. S., Jha, A., Pradhan-Sundd, T., Watkins, S. C., Owen, D. J., et al. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.05.003 · Cited by 115
Endocytic internalization routes required for delta/notch signaling
Windler, S. L., & Bilder, D. (2010) · Current Biology · doi:10.1016/j.cub.2010.01.049 · Cited by 113
Chen, Y., Gershlick, D. C., Park, S. Y., & Bonifacino, J. S. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201707172 · Cited by 102
Umasankar, P. K., Sanker, S., Thieman, J. R., Chakraborty, S., Wendland, B., Tsang, M., & Traub, L. M. (2012) · Nature Cell Biology · doi:10.1038/ncb2473 · Cited by 94
Kastning, K., Kukhtina, V., Kittler, J. T., Chen, G., Pechstein, A., Enders, S., Lee, S. H., Sheng, M., Yan, Z., & Haucke, V. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0611170104 · Cited by 88
Scotti, E., Calamai, M., Goulbourne, C. N., Zhang, L., Hong, C., Lin, R. R., Choi, J., Pilch, P. F., Fong, L. G., Zou, P., et al. (2013) · Molecular and Cellular Biology · doi:10.1128/mcb.01716-12 · Cited by 81
Cuitino, L., Matute, R., Retamal, C., Bu, G., Inestrosa, N. C., & Marzolo, M. (2005) · Traffic · doi:10.1111/j.1600-0854.2005.00320.x · Cited by 72
Sorrentino, V., Nelson, J. K., Maspero, E., Marques, A. R. A., Scheer, L., Polo, S., & Zelcer, N. (2013) · Journal of Lipid Research · doi:10.1194/jlr.m037713 · Cited by 68
Prabhu, Y., Burgos, P. V., Schindler, C., Farías, G. G., Magadán, J. G., & Bonifacino, J. S. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-11-0944 · Cited by 68
Chia, P. Z. C., Toh, W. H., Sharples, R., Gasnereau, I., Hill, A. F., & Gleeson, P. A. (2013) · Traffic · doi:10.1111/tra.12088 · Cited by 67
AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis
Gulbranson, D. R., Crisman, L., Lee, M., Ouyang, Y., Menasche, B. L., Demmitt, B. A., Wan, C., Nomura, T., Ye, Y., Yu, H., et al. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.06.013 · Cited by 57
Mishra, S. K., Keyel, P. A., Edeling, M. A., Dupin, A. L., Owen, D. J., & Traub, L. M. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m501029200 · Cited by 52
Contribution of the clathrin adaptor AP-1 subunit µ1 to acidic cluster protein sorting
Navarro Negredo, P., Edgar, J. R., Wrobel, A. G., Zaccai, N. R., Antrobus, R., Owen, D. J., & Robinson, M. S. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201602058 · Cited by 50
Self-citationMulkearns, E. E., & Cooper, J. A. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-09-0812 · Cited by 48
Borck, G., Mollà-Herman, A., Boddaert, N., Encha-Razavi, F., Philippe, A., Robel, L., Desguerre, I., Brunelle, F., Benmerah, A., Munnich, A., et al. (2008) · Human Mutation · doi:10.1002/humu.20531 · Cited by 44
Analysis of the AP-2 adaptor complex and cargo during clathrin-mediated endocytosis
Rappoport, J. Z., Benmerah, A., & Simon, S. M. (2005) · Traffic · doi:10.1111/j.1600-0854.2005.00280.x · Cited by 41
Eden, E. R., Sun, X.-M., Patel, D. D., & Soutar, A. K. (2007) · Human Molecular Genetics · doi:10.1093/hmg/ddm232 · Cited by 27
Cold shock domain-containing protein E1 is a posttranscriptional regulator of the LDL receptor
Smith, G. A., Padmanabhan, A., Lau, B. H., Pampana, A., Li, L., Lee, C. Y., Pelonero, A., Nishino, T., Sadagopan, N., Xia, V. Q., et al. (2022) · Science Translational Medicine · doi:10.1126/scitranslmed.abj8670 · Cited by 23
Matsudaira, T., Niki, T., Taguchi, T., & Arai, H. (2015) · Journal of Cell Science · doi:10.1242/jcs.172171 · Cited by 22
Duan, D., Hanson, M., Holland, D. O., & Johnson, M. E. (2022) · Scientific Reports · doi:10.1038/s41598-022-09259-w · Cited by 15
Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module
Wang, S., Chen, X., Crisman, L., Dou, X., Winborn, C. S., Wan, C., Puscher, H., Yin, Q., Kennedy, M. J., & Shen, J. (2023) · Science Advances · doi:10.1126/sciadv.ade2540 · Cited by 14
Metadata
- Authors
- Motley, A., Bright, N. A., Seaman, M. N. J., & Robinson, M. S.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-09-01
- Volume
- 162
- Issue
- 5
- Pages
- 909-918
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2032444338
- Cited by
- 697
- References
- 2
- FWCI
- 15.63
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
