Cited by 106Muscle metabolism and nutritionCardiovascular and exercise physiologySports Performance and Training

Dose-response effects of dietary protein on muscle protein synthesis during recovery from endurance exercise in young men: a double-blind randomized trial

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Churchward-Venne, Tyler A, Pinckaers, Philippe JM, Smeets, Joey SJ, Betz, Milan W, Senden, Joan M, Goessens, Joy PB, Gijsen, Annemie P, Rollo, Ian +2 more·2020·The American Journal of Clinical Nutrition

Abstract

BACKGROUND: Protein ingestion increases skeletal muscle protein synthesis rates during recovery from endurance exercise. OBJECTIVES: We aimed to determine the effect of graded doses of dietary protein co-ingested with carbohydrate on whole-body protein metabolism, and skeletal muscle myofibrillar (MyoPS) and mitochondrial (MitoPS) protein synthesis rates during recovery from endurance exercise. METHODS: In a randomized, double-blind, parallel-group design, 48 healthy, young, endurance-trained men (mean ± SEM age: 27 ± 1 y) received a primed continuous infusion of l-[ring-2H5]-phenylalanine, l-[ring-3,5-2H2]-tyrosine, and l-[1-13C]-leucine and ingested 45 g carbohydrate with either 0 (0 g PRO), 15 (15 g PRO), 30 (30 g PRO), or 45 (45 g PRO) g intrinsically l-[1-13C]-phenylalanine and l-[1-13C]-leucine labeled milk protein after endurance exercise. Blood and muscle biopsy samples were collected over 360 min of postexercise recovery to assess whole-body protein metabolism and both MyoPS and MitoPS rates. RESULTS: Protein intake resulted in ∼70%-74% of the ingested protein-derived phenylalanine appearing in the circulation. Whole-body net protein balance increased dose-dependently after ingestion of 0, 15, 30, or 45 g protein (mean ± SEM: -0.31± 0.16, 5.08 ± 0.21, 10.04 ± 0.30, and 13.49 ± 0.55 μmol phenylalanine · kg-1 · h-1, respectively; P < 0.001). 30 g PRO stimulated a ∼46% increase in MyoPS rates (%/h) compared with 0 g PRO and was sufficient to maximize MyoPS rates after endurance exercise. MitoPS rates were not increased after protein ingestion; however, incorporation of dietary protein-derived l-[1-13C]-phenylalanine into de novo mitochondrial protein increased dose-dependently after ingestion of 15, 30, and 45 g protein at 360 min postexercise (0.018 ± 0.002, 0.034 ± 0.002, and 0.046 ± 0.003 mole percentage excess, respectively; P < 0.001). CONCLUSIONS: Protein ingested after endurance exercise is efficiently digested and absorbed into the circulation. Whole-body net protein balance and dietary protein-derived amino acid incorporation into mitochondrial protein respond to increasing protein intake in a dose-dependent manner. Ingestion of 30 g protein is sufficient to maximize MyoPS rates during recovery from a single bout of endurance exercise.This trial was registered at trialregister.nl as NTR5111.

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Crossref · 10.1093/ajcn/nqaa073

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  • Co-ingestion of carbohydrates and protein during post-exercise may confer additional benefits, including positive nitrogen balance leading to increased whole-body muscle protein synthesis, increased protein content related to mitochondrial biogenesis, reduced muscle damage markers, and accelerated post-exercise rehydration.

    “it may confer additional benefits, including positive nitrogen balance leading to increased whole-body muscle protein synthesis [93], increased protein content related to mitochondrial biogenesis [94], reduced muscle damage markers [95], and accelerated post-exercise rehydration [96]”

    From Nutritional Strategies to Improve Post-exercise Recovery and Subsequent Exercise Performance: A Narrative Review (2025) · cites this paper as [93]

    The Discussion (page unavailable) reports that whole-body net protein balance was negative with carbohydrate alone but positive after protein co-ingestion. The Results also report higher myofibrillar protein synthesis with 30 or 45 g of protein. The paper does not establish that positive whole-body balance leads to increased muscle protein synthesis; its Discussion cautions that whole-body protein metabolism is not necessarily reflective of the skeletal-muscle response. The other benefits in the sentence have separate citations and are not assessed here. (LLM, conf=0.85)

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  • Muscle contraction in the form of resistance- or endurance-type exercise provides additional stimulus to upregulate MPS in both an additive and independent manner.

    “Muscle contraction in the form of resistance- or endurance-type exercise provides additional stimulus to upregulate MPS in both an additive and independent manner [[51], [52], [53]].”

    From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [52]

  • Marathon runners who consume moderate amounts of protein post-exercise can have recovery benefits.

    “Similarly, recent findings have provided evidence that marathon runners that consume moderate amounts of protein post-exercise can have recovery benefits [97,98].”

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Authors
Churchward-Venne, T. A., Pinckaers, P. J., Smeets, J. S., Betz, M. W., Senden, J. M., Goessens, J. P., Gijsen, A. P., Rollo, I., Verdijk, L. B., & van Loon, L. J.
Affiliations
Maastricht University Medical Centre (NL)
Venue
The American Journal of Clinical Nutrition
Publisher
Elsevier BV
Published
2020-08
Volume
112
Issue
2
Pages
303-317
ISSN
0002-9165 (ISSN-L)
OpenAlex
W3023379271
Cited by
106
References
50
Citations by year
2026: 9 · 2025: 20 · 2024: 15 · 2023: 19 · 2022: 15 · 2021: 20 · 2020: 8
FWCI
7.73
Type
journal article
Language
en
Open access
Locations
  • American Journal of Clinical Nutrition (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, cc-by-nc)
  • VUBIR (Vrije Universiteit Brussel) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion, cc-by-nc)
Funders
  • PepsiCo
  • Gatorade Sports Science Institute
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
Keywords
dietary protein, muscle protein synthesis, myofibrillar protein synthesis, mitochondrial translation, whole body protein metabolism, net protein balance, milk protein, phenylalanine incorporation, skeletal muscle, young men, endurance athletes, muscle hypertrophy, muscle atrophy
MeSH terms
Endurance Training, Adult, Amino Acids, Dietary Proteins, Double-Blind Method, Humans, Male, Muscle Proteins, Exercise, Muscle, Skeletal

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