Post-training reversible inactivation of the hippocampus enhances novel object recognition memory
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Research on the role of the hippocampus in object recognition memory has produced conflicting results. Previous studies have used permanent hippocampal lesions to assess the requirement for the hippocampus in the object recognition task. However, permanent hippocampal lesions may impact performance through effects on processes besides memory consolidation including acquisition, retrieval, and performance. To overcome this limitation, we used an intrahippocampal injection of the GABA agonist muscimol to reversibly inactivate the hippocampus immediately after training mice in two versions of an object recognition task. We found that the inactivation of the dorsal hippocampus after training impairs object-place recognition memory but enhances novel object recognition (NOR) memory. However, inactivation of the dorsal hippocampus after repeated exposure to the training context did not affect object recognition memory. Our findings suggest that object recognition memory formation does not require the hippocampus and, moreover, that activity in the hippocampus can interfere with the consolidation of object recognition memory when object information encoding occurs in an unfamiliar environment.
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Post-sample infusions of muscimol and the GABAa-R antagonist bicuculline into the dorsal hippocampus respectively facilitated and disrupted long-term NOP memory in a less-habituated environment, but not after sufficient habituation.
“Interestingly, post-sample infusions of muscimol and the GABAa-R antagonist bicuculline into the dorsal hippocampus respectively facilitated and disrupted long-term NOP memory in a less-habituated environment, but not after sufficient habituation (Kim et al., 2014; Oliveira et al., 2010).”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Oliveira et al., 2010
The hippocampus is undoubtedly important for NOP memory when the retention is over 10 minutes, while its involvement likely depends on contextual novelty, features of context, and probably allocentric signals.
“Thus, the hippocampus is undoubtedly important for NOP memory when the retention is over 10 minutes (Cohen and Stackman, 2015), while its involvement likely depends on contextual novelty (Kim et al., 2014; Oliveira et al., 2010; Yi et al., 2016), features of context (Piterkin et al., 2008), and probably allocentric signals (Langston and Wood, 2010) (see below).”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Oliveira et al., 2010
A non-effect of pharmacological inactivation on the dorsal hippocampus was reported in the NOP test if animals were well-habituated to the testing environment.
“However, a non-effect of pharmacological inactivation on the dorsal hippocampus was reported in the NOP test if animals were well-habituated to the testing environment (Kim et al., 2014; Oliveira et al., 2010).”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Oliveira et al., 2010
Post-sample trial muscimol or anisomycin microinjection into the hippocampus impaired long-term OPP memory.
“Post-sample trial muscimol or anisomycin microinjection into the hippocampus also impaired long-term OPP memory (Oliveira et al., 2010; Ozawa et al., 2017).”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Oliveira et al., 2010
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- Authors
- Oliveira, A. M. M., Hawk, J. D., Abel, T., & Havekes, R.
- Affiliations
- University of Pennsylvania (US)
- Venue
- Learning & Memory
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2010-02-26
- Volume
- 17
- Issue
- 3
- Pages
- 155-160
- ISSN
- 1072-0502 (ISSN-L)
- 1549-5485
- PMC
- PMC2832924
- OpenAlex
- W1967872727
- Cited by
- 222
- References
- 48
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 7 · 2023: 9 · 2022: 13 · 2021: 22 · 2020: 19 · 2019: 19 · 2018: 17 · 2017: 17 · 2016: 20 · 2015: 13 · 2014: 18 · 2013: 11 · 2012: 12
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Learning & Memory (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- hippocampus, novel object recognition memory, muscimol, dorsal hippocampus, memory consolidation, training context
- MeSH terms
- Analysis of Variance, Animals, Exploratory Behavior, Hippocampus, Male, Mice, Inbred C57BL, Motor Activity, Muscimol, Pattern Recognition, Visual, Photic Stimulation, Teaching, Time Factors, GABA Agonists, Recognition, Psychology, Mice
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