Leucine supplementation improves muscle protein synthesis in elderly men independently of hyperaminoacidaemia
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The present study was designed to assess the effects of dietary leucine supplementation on muscle protein synthesis and whole body protein kinetics in elderly individuals. Twenty healthy male subjects (70 +/- 1 years) were studied before and after continuous ingestion of a complete balanced diet supplemented or not with leucine. A primed (3.6 micromol kg(-1)) constant infusion (0.06 micromol kg(-1) min(-1)) of L-[1-13C]phenylalanine was used to determine whole body phenylalanine kinetics as well as fractional synthesis rate (FSR) in the myofibrillar fraction of muscle proteins from vastus lateralis biopsies. Whole body protein kinetics were not affected by leucine supplementation. In contrast, muscle FSR, measured over the 5-h period of feeding, was significantly greater in the volunteers given the leucine-supplemented meals compared with the control group (0.083 +/- 0.008 versus 0.053 +/- 0.009% h(-1), respectively, P < 0.05). This effect was due only to increased leucine availability because only plasma free leucine concentration significantly differed between the control and leucine-supplemented groups. We conclude that leucine supplementation during feeding improves muscle protein synthesis in the elderly independently of an overall increase of other amino acids. Whether increasing leucine intake in old people may limit muscle protein loss during ageing remains to be determined.
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Leucine supplementation during feeding improved muscle protein synthesis in older adults independently of an overall increase of other amino acids.
“Rieu 2006 62 Leucine supplementation during feeding improved muscle protein synthesis in older adults independently of an overall increase of other amino acids.”
From Evidence-Based Recommendations for Optimal Dietary Protein Intake in Older People: A Position Paper From the PROT-AGE Study Group (2013) · cites this paper as Rieu 2006 62
Leucine fortification has been shown, though not always, to augment the MPS response to suboptimal doses of animal protein.
“In line, leucine fortification has been shown [70,97,98], though not always [99], to augment the MPS response to suboptimal doses of animal protein.”
From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [97]
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- Authors
- Rieu, I., Balage, M., Sornet, C., Giraudet, C., Pujos, E., Grizard, J., Mosoni, L., & Dardevet, D.
- Affiliations
- Unité de Nutrition Humaine (FR)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2006-08-08
- Volume
- 575
- Issue
- 1
- Pages
- 305-315
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC1819434
- OpenAlex
- W1907574285
- Cited by
- 410
- References
- 55
- Citations by year
- 2026: 7 · 2025: 16 · 2024: 16 · 2023: 21 · 2022: 18 · 2021: 18 · 2020: 16 · 2019: 18 · 2018: 23 · 2017: 15 · 2016: 27 · 2015: 25 · 2014: 18 · 2013: 32 · 2012: 39
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- European Society for Clinical Nutrition and Metabolism
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- leucine supplementation, muscle protein synthesis, older men, hyperaminoacidemia, phenylalanine kinetics, fractional synthesis rate, myofibrillar proteins, muscle protein loss, aging
- MeSH terms
- Aged, Amino Acids, Breath Tests, Carbon Dioxide, Carbon Radioisotopes, Humans, Insulin, Leucine, Male, Muscle Proteins, Phenylalanine, Muscle, Skeletal, Postprandial Period, Dietary Supplements
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