Cited by 410Muscle metabolism and nutritionNutrition and Health in AgingMuscle Physiology and Disorders

Leucine supplementation improves muscle protein synthesis in elderly men independently of hyperaminoacidaemia

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Rieu, Isabelle, Balage, Michèle, Sornet, Claire, Giraudet, Christophe, Pujos, Estelle, Grizard, Jean, Mosoni, Laurent, Dardevet, Dominique·2006·The Journal of Physiology

Abstract

The present study was designed to assess the effects of dietary leucine supplementation on muscle protein synthesis and whole body protein kinetics in elderly individuals. Twenty healthy male subjects (70 +/- 1 years) were studied before and after continuous ingestion of a complete balanced diet supplemented or not with leucine. A primed (3.6 micromol kg(-1)) constant infusion (0.06 micromol kg(-1) min(-1)) of L-[1-13C]phenylalanine was used to determine whole body phenylalanine kinetics as well as fractional synthesis rate (FSR) in the myofibrillar fraction of muscle proteins from vastus lateralis biopsies. Whole body protein kinetics were not affected by leucine supplementation. In contrast, muscle FSR, measured over the 5-h period of feeding, was significantly greater in the volunteers given the leucine-supplemented meals compared with the control group (0.083 +/- 0.008 versus 0.053 +/- 0.009% h(-1), respectively, P < 0.05). This effect was due only to increased leucine availability because only plasma free leucine concentration significantly differed between the control and leucine-supplemented groups. We conclude that leucine supplementation during feeding improves muscle protein synthesis in the elderly independently of an overall increase of other amino acids. Whether increasing leucine intake in old people may limit muscle protein loss during ageing remains to be determined.

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Crossref · 10.1113/jphysiol.2006.110742

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Authors
Rieu, I., Balage, M., Sornet, C., Giraudet, C., Pujos, E., Grizard, J., Mosoni, L., & Dardevet, D.
Affiliations
Unité de Nutrition Humaine (FR)
Venue
The Journal of Physiology
Publisher
Wiley
Published
2006-08-08
Volume
575
Issue
1
Pages
305-315
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
OpenAlex
W1907574285
Cited by
410
References
55
Citations by year
2026: 7 · 2025: 16 · 2024: 16 · 2023: 21 · 2022: 18 · 2021: 18 · 2020: 16 · 2019: 18 · 2018: 23 · 2017: 15 · 2016: 27 · 2015: 25 · 2014: 18 · 2013: 32 · 2012: 39
FWCI
6.77
Type
journal article
Language
en
Open access
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
European Society for Clinical Nutrition and Metabolism
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
leucine supplementation, muscle protein synthesis, older men, hyperaminoacidemia, phenylalanine kinetics, fractional synthesis rate, myofibrillar proteins, muscle protein loss, aging
MeSH terms
Aged, Amino Acids, Breath Tests, Carbon Dioxide, Carbon Radioisotopes, Humans, Insulin, Leucine, Male, Muscle Proteins, Phenylalanine, Muscle, Skeletal, Postprandial Period, Dietary Supplements

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